Anticancer effects of silver nanoparticles encapsulated by Taxus baccata extracts

Kajani, AA; Zarkesh-Esfahani, SH; Bordbar, AK; Khosropour, AR; Razmjou, A; Kardi, M

Anticancer effects of silver nanoparticles encapsulated by Taxus baccata extracts

Kajani, AA Zarkesh-Esfahani, SH Bordbar, AK Khosropour, AR Razmjou, A Kardi, M

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Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: Journal of Molecular Liquids, 2016, 223, pp. 549-556
Issue Date:: 2016-11-01

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Field	Value	Language
dc.contributor.author	Kajani, AA
dc.contributor.author	Zarkesh-Esfahani, SH
dc.contributor.author	Bordbar, AK
dc.contributor.author	Khosropour, AR
dc.contributor.author	Razmjou, A
dc.contributor.author	Kardi, M
dc.date.accessioned	2023-03-27T00:07:40Z
dc.date.available	2023-03-27T00:07:40Z
dc.date.issued	2016-11-01
dc.identifier.citation	Journal of Molecular Liquids, 2016, 223, pp. 549-556
dc.identifier.issn	0167-7322
dc.identifier.issn	1873-3166
dc.identifier.uri	http://hdl.handle.net/10453/168490
dc.description.abstract	Cancer is one of the main causes of human fatality and finding effective anticancer drugs is a high priority. Modified anticancer medicines have advantages compared to traditional drugs including; better delivery and lower doses. In the present study, the anticancer activity of silver nanoparticles (Ag NPs) synthesized using Taxus baccata extracts was studied on Caov-4 and HeLa cancer cell lines as well as normal human fibroblast cells. Microscopic studies showed significant morphological changes of cancer cells following the exposure to the Ag NPs while MTT assay revealed dose, time and cell line dependent cytotoxicity. The toxic effect of Ag NPs on Caov-4 cells was considerably higher than HeLa cells as > 98% mortality obtained after 72 h incubation of Caov-4 cells with 5 and 20 μg/mL Ag NPs encapsulated by aqueous and ethanolic extract, respectively. Interestingly, aqueous extract encapsulated Ag NPs had no significant toxicity on normal fibroblast cells suggesting potent and selective anticancer activity on cancer cell lines. Flow cytometric analysis revealed a combination of apoptosis and necrosis following the exposure of Ag NPs to cancer cells. Apoptosis was determined as main mechanism of cell death in low concentration of Ag NPs while considerable necrosis (up to 41%) was observed by increasing the Ag NPs dose (up to 10 μg/mL) and incubation time (up to 72 h). The significant DNA damage and change in expression level of caspase 8, caspase 9, bcl-2 and c-Abl genes indicated the induction of mitochondrial death pathway following cell exposure to Ag NPs. It can be concluded that the preparation method and stabilizing agents may have major effects on the biological activity of nanoparticles as well as their physicochemical properties.
dc.language	English
dc.publisher	ELSEVIER
dc.relation.ispartof	Journal of Molecular Liquids
dc.relation.isbasedon	10.1016/j.molliq.2016.08.064
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0306 Physical Chemistry (incl. Structural)
dc.subject.classification	Chemical Physics
dc.title	Anticancer effects of silver nanoparticles encapsulated by Taxus baccata extracts
dc.type	Journal Article
utslib.citation.volume	223
utslib.for	0306 Physical Chemistry (incl. Structural)
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2023-03-27T00:07:38Z
pubs.publication-status	Published
pubs.volume	223

Abstract:

Cancer is one of the main causes of human fatality and finding effective anticancer drugs is a high priority. Modified anticancer medicines have advantages compared to traditional drugs including; better delivery and lower doses. In the present study, the anticancer activity of silver nanoparticles (Ag NPs) synthesized using Taxus baccata extracts was studied on Caov-4 and HeLa cancer cell lines as well as normal human fibroblast cells. Microscopic studies showed significant morphological changes of cancer cells following the exposure to the Ag NPs while MTT assay revealed dose, time and cell line dependent cytotoxicity. The toxic effect of Ag NPs on Caov-4 cells was considerably higher than HeLa cells as > 98% mortality obtained after 72 h incubation of Caov-4 cells with 5 and 20 μg/mL Ag NPs encapsulated by aqueous and ethanolic extract, respectively. Interestingly, aqueous extract encapsulated Ag NPs had no significant toxicity on normal fibroblast cells suggesting potent and selective anticancer activity on cancer cell lines. Flow cytometric analysis revealed a combination of apoptosis and necrosis following the exposure of Ag NPs to cancer cells. Apoptosis was determined as main mechanism of cell death in low concentration of Ag NPs while considerable necrosis (up to 41%) was observed by increasing the Ag NPs dose (up to 10 μg/mL) and incubation time (up to 72 h). The significant DNA damage and change in expression level of caspase 8, caspase 9, bcl-2 and c-Abl genes indicated the induction of mitochondrial death pathway following cell exposure to Ag NPs. It can be concluded that the preparation method and stabilizing agents may have major effects on the biological activity of nanoparticles as well as their physicochemical properties.

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http://hdl.handle.net/10453/168490