RNA modulators of complex phenotypes in mammalian cells

Lai, A; Cairns, MJ; Tran, NT; Zhang, H; Cullen, L; Arndt, GM

RNA modulators of complex phenotypes in mammalian cells

Lai, A Cairns, MJ Tran, NT

Zhang, H Cullen, L Arndt, GM

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Publisher:: Public library of Science
Publication Type:: Journal Article
Citation:: PLoS ONE, 2009, 487 (3)
Issue Date:: 2009-01

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Field	Value	Language
dc.contributor.author	Lai, A	en_US
dc.contributor.author	Cairns, MJ	en_US
dc.contributor.author	Tran, NT https://orcid.org/0000-0001-7747-2530	en_US
dc.contributor.author	Zhang, H	en_US
dc.contributor.author	Cullen, L	en_US
dc.contributor.author	Arndt, GM	en_US
dc.date.issued	2009-01	en_US
dc.identifier.citation	PLoS ONE, 2009, 487 (3)	en_US
dc.identifier.issn	1932-6203	en_US
dc.identifier.uri	http://hdl.handle.net/10453/16871
dc.description.abstract	RNA-mediated gene silencing, in the form of RNA interference (RNAi) or microRNAs (miRNAs) has provided novel tools for gene discovery and validation in mammalian cells. Here, we report on the construction and application of a random small RNA expression library for use in identifying small interfering RNA (siRNA) effectors that can modify complex cellular phenotypes in mammalian cells. The library is based in a retroviral vector and uses convergent promoters to produce unique small complementary RNAs. Using this library, we identify a range of small RNA-encoding gene inserts that overcome resistance to 5-fluorouracil (5-FU)- or tumour necrosis factor alpha (TNF-a)- induced cell death in colorectal cancer cells. We demonstrate the utility of this technology platform by identifying a key RNA effector, in the form of a siRNA, which overcomes cell death induced by the chemotherapeutic 5-FU. The technology described has the potential to identify both functional RNA modulators capable of altering physiological systems and the cellular target genes altered by these modulators.	en_US
dc.publisher	Public library of Science	en_US
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0062305	en_US
dc.subject.classification	General Science & Technology	en_US
dc.title	RNA modulators of complex phenotypes in mammalian cells	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	487	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.consider-herdc	false	en_US
pubs.issue	3	en_US
pubs.volume	487	en_US

Abstract:

RNA-mediated gene silencing, in the form of RNA interference (RNAi) or microRNAs (miRNAs) has provided novel tools for gene discovery and validation in mammalian cells. Here, we report on the construction and application of a random small RNA expression library for use in identifying small interfering RNA (siRNA) effectors that can modify complex cellular phenotypes in mammalian cells. The library is based in a retroviral vector and uses convergent promoters to produce unique small complementary RNAs. Using this library, we identify a range of small RNA-encoding gene inserts that overcome resistance to 5-fluorouracil (5-FU)- or tumour necrosis factor alpha (TNF-a)- induced cell death in colorectal cancer cells. We demonstrate the utility of this technology platform by identifying a key RNA effector, in the form of a siRNA, which overcomes cell death induced by the chemotherapeutic 5-FU. The technology described has the potential to identify both functional RNA modulators capable of altering physiological systems and the cellular target genes altered by these modulators.

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http://hdl.handle.net/10453/16871