Inactivation of antibiotic resistant bacterium Escherichia coli by electrochemical disinfection on molybdenum carbide electrode.

Liu, Y; Zhang, S; Fang, H; Wang, Q; Jiang, S; Zhang, C; Qiu, P

Inactivation of antibiotic resistant bacterium Escherichia coli by electrochemical disinfection on molybdenum carbide electrode.

Liu, Y Zhang, S Fang, H Wang, Q

Jiang, S Zhang, C Qiu, P

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Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Chemosphere, 2022, 287, (Pt 4), pp. 132398-132398
Issue Date:: 2022-01-01

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Field	Value	Language
dc.contributor.author	Liu, Y
dc.contributor.author	Zhang, S
dc.contributor.author	Fang, H
dc.contributor.author	Wang, Q https://orcid.org/0000-0002-5744-2331
dc.contributor.author	Jiang, S
dc.contributor.author	Zhang, C
dc.contributor.author	Qiu, P
dc.date.accessioned	2023-04-05T04:47:15Z
dc.date.available	2021-09-27
dc.date.available	2023-04-05T04:47:15Z
dc.date.issued	2022-01-01
dc.identifier.citation	Chemosphere, 2022, 287, (Pt 4), pp. 132398-132398
dc.identifier.issn	0045-6535
dc.identifier.issn	1879-1298
dc.identifier.uri	http://hdl.handle.net/10453/169195
dc.description.abstract	Antibiotic-resistant bacteria (ARB) pose a substantial threat to public health worldwide. Electrochemistry, as a low energy consumption and environmentally friendly technique, is ideal for inactivating ARB. This study explored the utility of electrochemical disinfection (ED) for inactivating ARB (Escherichia coli K-12 LE392 resistant to kanamycin, tetracycline, and ampicillin) and the regrowth potential of the treated ARB. The results revealed that 5.12-log ARB removal was achieved within 30 min of applying molybdenum carbide as the anode and cathode material under a voltage of 2.0 V. No ARB regrowth was observed in the cathode chamber after 60 min of incubation in unselective broth, demonstrating that the process in the cathode chamber was more effective for permanent inactivation of ARB. The mechanisms underlying the ARB inactivation were verified based on intercellular reactive oxygen species (ROS) measurement, membrane integrity detection, and genetic damage assessment. Higher ROS production and membrane permeability were observed in the cathode and anode groups (p < 0.001) compared to the control group (0 V). In addition, the DNA was more likely to be damaged during the ED process. Collectively, our results demonstrate that ED is a promising technology for disinfecting water to prevent the spread of ARB.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation	http://purl.org/au-research/grants/arc/FT200100264
dc.relation.ispartof	Chemosphere
dc.relation.isbasedon	10.1016/j.chemosphere.2021.132398
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Sciences
dc.subject.classification	Meteorology & Atmospheric Sciences
dc.subject.mesh	Angiotensin Receptor Antagonists
dc.subject.mesh	Angiotensin-Converting Enzyme Inhibitors
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Bacteria
dc.subject.mesh	Disinfection
dc.subject.mesh	Electrodes
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Escherichia coli K12
dc.subject.mesh	Molybdenum
dc.subject.mesh	Waste Water
dc.subject.mesh	Angiotensin Receptor Antagonists
dc.subject.mesh	Angiotensin-Converting Enzyme Inhibitors
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Bacteria
dc.subject.mesh	Disinfection
dc.subject.mesh	Electrodes
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Escherichia coli K12
dc.subject.mesh	Molybdenum
dc.subject.mesh	Wastewater
dc.subject.mesh	Bacteria
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Escherichia coli K12
dc.subject.mesh	Molybdenum
dc.subject.mesh	Angiotensin-Converting Enzyme Inhibitors
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Electrodes
dc.subject.mesh	Disinfection
dc.subject.mesh	Angiotensin Receptor Antagonists
dc.subject.mesh	Wastewater
dc.subject.mesh	Angiotensin Receptor Antagonists
dc.subject.mesh	Angiotensin-Converting Enzyme Inhibitors
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Bacteria
dc.subject.mesh	Disinfection
dc.subject.mesh	Electrodes
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Escherichia coli K12
dc.subject.mesh	Molybdenum
dc.subject.mesh	Wastewater
dc.title	Inactivation of antibiotic resistant bacterium Escherichia coli by electrochemical disinfection on molybdenum carbide electrode.
dc.type	Journal Article
utslib.citation.volume	287
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2023-04-05T04:47:14Z
pubs.issue	Pt 4
pubs.publication-status	Published
pubs.volume	287
utslib.citation.issue	Pt 4

Abstract:

Antibiotic-resistant bacteria (ARB) pose a substantial threat to public health worldwide. Electrochemistry, as a low energy consumption and environmentally friendly technique, is ideal for inactivating ARB. This study explored the utility of electrochemical disinfection (ED) for inactivating ARB (Escherichia coli K-12 LE392 resistant to kanamycin, tetracycline, and ampicillin) and the regrowth potential of the treated ARB. The results revealed that 5.12-log ARB removal was achieved within 30 min of applying molybdenum carbide as the anode and cathode material under a voltage of 2.0 V. No ARB regrowth was observed in the cathode chamber after 60 min of incubation in unselective broth, demonstrating that the process in the cathode chamber was more effective for permanent inactivation of ARB. The mechanisms underlying the ARB inactivation were verified based on intercellular reactive oxygen species (ROS) measurement, membrane integrity detection, and genetic damage assessment. Higher ROS production and membrane permeability were observed in the cathode and anode groups (p < 0.001) compared to the control group (0 V). In addition, the DNA was more likely to be damaged during the ED process. Collectively, our results demonstrate that ED is a promising technology for disinfecting water to prevent the spread of ARB.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/169195