Recent advances in nanotechnology approaches for non-viral gene therapy.

Jiang, Y; Fan, M; Yang, Z; Liu, X; Xu, Z; Liu, S; Feng, G; Tang, S; Li, Z; Zhang, Y; Chen, S; Yang, C; Law, W-C; Dong, B; Xu, G; Yong, K-T

Recent advances in nanotechnology approaches for non-viral gene therapy.

Jiang, Y Fan, M Yang, Z

Liu, X Xu, Z Liu, S Feng, G Tang, S Li, Z Zhang, Y Chen, S Yang, C Law, W-C Dong, B Xu, G Yong, K-T

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Publisher:: Royal Society of Chemistry (RSC)
Publication Type:: Journal Article
Citation:: Biomater Sci, 2022, 10, (24), pp. 6862-6892
Issue Date:: 2022-12-06

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Field	Value	Language
dc.contributor.author	Jiang, Y
dc.contributor.author	Fan, M
dc.contributor.author	Yang, Z https://orcid.org/0000-0003-1335-6893
dc.contributor.author	Liu, X
dc.contributor.author	Xu, Z
dc.contributor.author	Liu, S
dc.contributor.author	Feng, G
dc.contributor.author	Tang, S
dc.contributor.author	Li, Z
dc.contributor.author	Zhang, Y
dc.contributor.author	Chen, S
dc.contributor.author	Yang, C
dc.contributor.author	Law, W-C
dc.contributor.author	Dong, B
dc.contributor.author	Xu, G
dc.contributor.author	Yong, K-T
dc.date.accessioned	2023-04-13T00:10:48Z
dc.date.available	2023-04-13T00:10:48Z
dc.date.issued	2022-12-06
dc.identifier.citation	Biomater Sci, 2022, 10, (24), pp. 6862-6892
dc.identifier.issn	2047-4830
dc.identifier.issn	2047-4849
dc.identifier.uri	http://hdl.handle.net/10453/169700
dc.description.abstract	Gene therapy has shown great potential in the treatment of many diseases by downregulating the expression of certain genes. The development of gene vectors as a vehicle for gene therapy has greatly facilitated the widespread clinical application of nucleic acid materials (DNA, mRNA, siRNA, and miRNA). Currently, both viral and non-viral vectors are used as delivery systems of nucleic acid materials for gene therapy. However, viral vector-based gene therapy has several limitations, including immunogenicity and carcinogenesis caused by the exogenous viral vectors. To address these issues, non-viral nanocarrier-based gene therapy has been explored for superior performance with enhanced gene stability, high treatment efficiency, improved tumor-targeting, and better biocompatibility. In this review, we discuss various non-viral vector-mediated gene therapy approaches using multifunctional biodegradable or non-biodegradable nanocarriers, including polymer-based nanoparticles, lipid-based nanoparticles, carbon nanotubes, gold nanoparticles (AuNPs), quantum dots (QDs), silica nanoparticles, metal-based nanoparticles and two-dimensional nanocarriers. Various strategies to construct non-viral nanocarriers based on their delivery efficiency of targeted genes will be introduced. Subsequently, we discuss the cellular uptake pathways of non-viral nanocarriers. In addition, multifunctional gene therapy based on non-viral nanocarriers is summarized, in which the gene therapy can be combined with other treatments, such as photothermal therapy (PTT), photodynamic therapy (PDT), immunotherapy and chemotherapy. We also provide a comprehensive discussion of the biological toxicity and safety of non-viral vector-based gene therapy. Finally, the present limitations and challenges of non-viral nanocarriers for gene therapy in future clinical research are discussed, to promote wider clinical applications of non-viral vector-based gene therapy.
dc.format	Electronic
dc.language	eng
dc.publisher	Royal Society of Chemistry (RSC)
dc.relation.ispartof	Biomater Sci
dc.relation.isbasedon	10.1039/d2bm01001a
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0304 Medicinal and Biomolecular Chemistry, 0601 Biochemistry and Cell Biology, 1004 Medical Biotechnology
dc.subject.mesh	Gold
dc.subject.mesh	Nanotubes, Carbon
dc.subject.mesh	Metal Nanoparticles
dc.subject.mesh	Genetic Therapy
dc.subject.mesh	Nucleic Acids
dc.subject.mesh	Nanotubes, Carbon
dc.subject.mesh	Gold
dc.subject.mesh	Nucleic Acids
dc.subject.mesh	Metal Nanoparticles
dc.subject.mesh	Genetic Therapy
dc.subject.mesh	Gold
dc.subject.mesh	Nanotubes, Carbon
dc.subject.mesh	Metal Nanoparticles
dc.subject.mesh	Genetic Therapy
dc.subject.mesh	Nucleic Acids
dc.title	Recent advances in nanotechnology approaches for non-viral gene therapy.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	England
utslib.for	0304 Medicinal and Biomolecular Chemistry
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	1004 Medical Biotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
utslib.copyright.status	closed_access	*
dc.date.updated	2023-04-13T00:10:47Z
pubs.issue	24
pubs.publication-status	Published online
pubs.volume	10
utslib.citation.issue	24

Abstract:

Gene therapy has shown great potential in the treatment of many diseases by downregulating the expression of certain genes. The development of gene vectors as a vehicle for gene therapy has greatly facilitated the widespread clinical application of nucleic acid materials (DNA, mRNA, siRNA, and miRNA). Currently, both viral and non-viral vectors are used as delivery systems of nucleic acid materials for gene therapy. However, viral vector-based gene therapy has several limitations, including immunogenicity and carcinogenesis caused by the exogenous viral vectors. To address these issues, non-viral nanocarrier-based gene therapy has been explored for superior performance with enhanced gene stability, high treatment efficiency, improved tumor-targeting, and better biocompatibility. In this review, we discuss various non-viral vector-mediated gene therapy approaches using multifunctional biodegradable or non-biodegradable nanocarriers, including polymer-based nanoparticles, lipid-based nanoparticles, carbon nanotubes, gold nanoparticles (AuNPs), quantum dots (QDs), silica nanoparticles, metal-based nanoparticles and two-dimensional nanocarriers. Various strategies to construct non-viral nanocarriers based on their delivery efficiency of targeted genes will be introduced. Subsequently, we discuss the cellular uptake pathways of non-viral nanocarriers. In addition, multifunctional gene therapy based on non-viral nanocarriers is summarized, in which the gene therapy can be combined with other treatments, such as photothermal therapy (PTT), photodynamic therapy (PDT), immunotherapy and chemotherapy. We also provide a comprehensive discussion of the biological toxicity and safety of non-viral vector-based gene therapy. Finally, the present limitations and challenges of non-viral nanocarriers for gene therapy in future clinical research are discussed, to promote wider clinical applications of non-viral vector-based gene therapy.

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http://hdl.handle.net/10453/169700