Field |
Value |
Language |
dc.contributor.author |
Harapan, H |
|
dc.contributor.author |
Johar, E |
|
dc.contributor.author |
Maroef, CN |
|
dc.contributor.author |
Sriyani, IY |
|
dc.contributor.author |
Iqhrammullah, M |
|
dc.contributor.author |
Kusuma, HI |
|
dc.contributor.author |
Syukri, M |
|
dc.contributor.author |
Razali, R |
|
dc.contributor.author |
Hamdani, H |
|
dc.contributor.author |
Kurniawan, R |
|
dc.contributor.author |
Irwansyah, I |
|
dc.contributor.author |
Sofyan, SE |
|
dc.contributor.author |
Myint, KS |
|
dc.contributor.author |
Mahlia, TMI |
|
dc.contributor.author |
Rizal, S |
|
dc.date.accessioned |
2023-10-26T22:35:53Z |
|
dc.date.available |
2023-10-26T22:35:53Z |
|
dc.identifier.citation |
F1000Research, 11, pp. 403 |
|
dc.identifier.issn |
2046-1402 |
|
dc.identifier.issn |
2046-1402 |
|
dc.identifier.uri |
http://hdl.handle.net/10453/172867
|
|
dc.description.abstract |
<ns4:p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 10<ns4:sup>13</ns4:sup> PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 10<ns4:sup>13</ns4:sup> PFU/mL to 10<ns4:sup>9</ns4:sup> PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.</ns4:p> |
|
dc.language |
en |
|
dc.publisher |
Faculty of 1000 |
|
dc.relation |
University of Technology Sydney |
|
dc.relation.ispartof |
F1000Research |
|
dc.relation.isbasedon |
10.12688/f1000research.110305.2 |
|
dc.rights |
info:eu-repo/semantics/openAccess |
|
dc.subject |
0601 Biochemistry and Cell Biology, 1103 Clinical Sciences, 1112 Oncology and Carcinogenesis |
|
dc.subject.mesh |
Humans |
|
dc.subject.mesh |
SARS-CoV-2 |
|
dc.subject.mesh |
COVID-19 |
|
dc.subject.mesh |
Temperature |
|
dc.subject.mesh |
Hyperthermia, Induced |
|
dc.title |
Effect of elevated temperature on SARS-CoV-2 viability |
|
dc.type |
Journal Article |
|
utslib.citation.volume |
11 |
|
utslib.for |
0601 Biochemistry and Cell Biology |
|
utslib.for |
1103 Clinical Sciences |
|
utslib.for |
1112 Oncology and Carcinogenesis |
|
pubs.organisational-group |
/University of Technology Sydney |
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Engineering and Information Technology |
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering |
|
pubs.organisational-group |
/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment |
|
utslib.copyright.status |
open_access |
* |
dc.date.updated |
2023-10-26T22:35:51Z |
|
pubs.publication-status |
Published online |
|
pubs.volume |
11 |
|