Quality by Design-based RP-HPLC Method for Estimation of Curcumin in Rat Plasma and Fecal Microbiota Extract-based Solid Self-nano Emulsifying Drug Delivery System.

Corrie, L; Gulati, M; Kaur, J; Awasthi, A; Vishwas, S; Ramanunny, AK; Khursheed, R; Dua, K; Chellappan, DK; Singh, SK

Quality by Design-based RP-HPLC Method for Estimation of Curcumin in Rat Plasma and Fecal Microbiota Extract-based Solid Self-nano Emulsifying Drug Delivery System.

Corrie, L Gulati, M Kaur, J Awasthi, A Vishwas, S Ramanunny, AK Khursheed, R Dua, K

Chellappan, DK Singh, SK

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Publisher:: Bentham Science Publishers
Publication Type:: Journal Article
Citation:: Curr Drug Res Rev, 2023, 15, (3), pp. 272-285
Issue Date:: 2023

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Field	Value	Language
dc.contributor.author	Corrie, L
dc.contributor.author	Gulati, M
dc.contributor.author	Kaur, J
dc.contributor.author	Awasthi, A
dc.contributor.author	Vishwas, S
dc.contributor.author	Ramanunny, AK
dc.contributor.author	Khursheed, R
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.contributor.author	Chellappan, DK
dc.contributor.author	Singh, SK
dc.date.accessioned	2024-01-31T23:23:27Z
dc.date.available	2022-11-29
dc.date.available	2024-01-31T23:23:27Z
dc.date.issued	2023
dc.identifier.citation	Curr Drug Res Rev, 2023, 15, (3), pp. 272-285
dc.identifier.issn	2589-9775
dc.identifier.issn	2589-9783
dc.identifier.uri	http://hdl.handle.net/10453/175161
dc.description.abstract	BACKGROUND: Curcumin (CRM) is known to possess various therapeutic properties, such as anti-inflammatory and antidiabetic properties, and is, therefore, considered to be an effective therapeutic. OBJECTIVE: A sensitive method for the estimation of CRM in plasma, as well as fecal matter-based solid self-nano emulsifying drug delivery system (S-SNEDDS), has been reported for the first time. METHODS: A bioanalytical method was optimized using Box-Behnken Design having 13 runs and 3 responses. The optimized method was developed using methanol and water (70:30 v/v) with a flow rate of 1 mL/min. Quercetin was used as an internal standard. A specificity test was also performed for the developed CRM solid self-nano emulsifying drug delivery system. RESULTS: The retention time of CRM was found to be 14.18 minutes. The developed method was validated and found to be linear in the range of 50-250 ng/mL with an R2 of 0.999. Accuracy studies indicated that CRM had a percentage recovery of less than 105% and more than 95%, respectively. Precision studies were carried out for inter, intraday, and inter-analyst precision, and the %RSD was found to be less than 2%. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 3.37 ng/mL and 10.23 ng/mL, respectively. Stability studies for shortterm, long term and freeze-thaw cycles showed a %RSD of less than 2%, indicating the stability of CRM in the plasma matrix. Moreover, the blank fecal microbiota extract slurry did not show any peak at the retention time of CRM in a CRM-loaded solid nanoemulsifying drug delivery system containing fecal microbiota extract indicating its specificity. CONCLUSION: Hence, the developed method can have clinical implications as it helps estimate CRM in blood samples and also provides a simple and sensitive method for the estimation of plant-based flavonoids along with fecal microbiota extract formulations.
dc.format	Print
dc.language	eng
dc.publisher	Bentham Science Publishers
dc.relation.ispartof	Curr Drug Res Rev
dc.relation.isbasedon	10.2174/2589977515666230120140543
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.mesh	Rats
dc.subject.mesh	Animals
dc.subject.mesh	Chromatography, High Pressure Liquid
dc.subject.mesh	Curcumin
dc.subject.mesh	Limit of Detection
dc.subject.mesh	Drug Compounding
dc.subject.mesh	Drug Delivery Systems
dc.subject.mesh	Animals
dc.subject.mesh	Rats
dc.subject.mesh	Curcumin
dc.subject.mesh	Drug Delivery Systems
dc.subject.mesh	Chromatography, High Pressure Liquid
dc.subject.mesh	Drug Compounding
dc.subject.mesh	Limit of Detection
dc.subject.mesh	Rats
dc.subject.mesh	Animals
dc.subject.mesh	Chromatography, High Pressure Liquid
dc.subject.mesh	Curcumin
dc.subject.mesh	Limit of Detection
dc.subject.mesh	Drug Compounding
dc.subject.mesh	Drug Delivery Systems
dc.title	Quality by Design-based RP-HPLC Method for Estimation of Curcumin in Rat Plasma and Fecal Microbiota Extract-based Solid Self-nano Emulsifying Drug Delivery System.
dc.type	Journal Article
utslib.citation.volume	15
utslib.location.activity	United Arab Emirates
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Health
pubs.organisational-group	University of Technology Sydney/Faculty of Health/Public Health
utslib.copyright.status	closed_access	*
dc.date.updated	2024-01-31T23:23:25Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	15
utslib.citation.issue	3

Abstract:

BACKGROUND: Curcumin (CRM) is known to possess various therapeutic properties, such as anti-inflammatory and antidiabetic properties, and is, therefore, considered to be an effective therapeutic. OBJECTIVE: A sensitive method for the estimation of CRM in plasma, as well as fecal matter-based solid self-nano emulsifying drug delivery system (S-SNEDDS), has been reported for the first time. METHODS: A bioanalytical method was optimized using Box-Behnken Design having 13 runs and 3 responses. The optimized method was developed using methanol and water (70:30 v/v) with a flow rate of 1 mL/min. Quercetin was used as an internal standard. A specificity test was also performed for the developed CRM solid self-nano emulsifying drug delivery system. RESULTS: The retention time of CRM was found to be 14.18 minutes. The developed method was validated and found to be linear in the range of 50-250 ng/mL with an R2 of 0.999. Accuracy studies indicated that CRM had a percentage recovery of less than 105% and more than 95%, respectively. Precision studies were carried out for inter, intraday, and inter-analyst precision, and the %RSD was found to be less than 2%. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 3.37 ng/mL and 10.23 ng/mL, respectively. Stability studies for shortterm, long term and freeze-thaw cycles showed a %RSD of less than 2%, indicating the stability of CRM in the plasma matrix. Moreover, the blank fecal microbiota extract slurry did not show any peak at the retention time of CRM in a CRM-loaded solid nanoemulsifying drug delivery system containing fecal microbiota extract indicating its specificity. CONCLUSION: Hence, the developed method can have clinical implications as it helps estimate CRM in blood samples and also provides a simple and sensitive method for the estimation of plant-based flavonoids along with fecal microbiota extract formulations.

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http://hdl.handle.net/10453/175161