Exposure of sulfur-driven autotrophic denitrification to hydroxylamine/hydrazine: Underlying mechanisms and implications for promoting partial denitrification and N<inf>2</inf>O recovery

Huo, P; Deng, R; Yang, L; Liu, Y; Wei, W; Ni, BJ; Chen, X

Exposure of sulfur-driven autotrophic denitrification to hydroxylamine/hydrazine: Underlying mechanisms and implications for promoting partial denitrification and N<inf>2</inf>O recovery

Huo, P Deng, R Yang, L Liu, Y

Wei, W Ni, BJ Chen, X

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Publisher:: ELSEVIER SCIENCE SA
Publication Type:: Journal Article
Citation:: Chemical Engineering Journal, 2023, 477
Issue Date:: 2023-12-01

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Field	Value	Language
dc.contributor.author	Huo, P
dc.contributor.author	Deng, R
dc.contributor.author	Yang, L
dc.contributor.author	Liu, Y https://orcid.org/0000-0001-6677-7961
dc.contributor.author	Wei, W
dc.contributor.author	Ni, BJ
dc.contributor.author	Chen, X
dc.date.accessioned	2024-03-11T01:10:48Z
dc.date.available	2024-03-11T01:10:48Z
dc.date.issued	2023-12-01
dc.identifier.citation	Chemical Engineering Journal, 2023, 477
dc.identifier.issn	1385-8947
dc.identifier.issn	1873-3212
dc.identifier.uri	http://hdl.handle.net/10453/176431
dc.description.abstract	Hydroxylamine (NH2OH) and hydrazine (N2H4) are crucial intermediates in biological nitrogen removal processes, but their potential effects on the nitrogen-related metabolic pathway during the sulfur-driven autotrophic denitrification (SDAD) process remain unclear and were thus explored in this work. The results of dedicated batch tests indicated that the accumulation ratio of NO2− was positively correlated with the NH2OH concentration, reaching 86.2 ± 2.6% at the NH2OH concentration of 2.0 mg-N/L, while it was barely affected by the N2H4 concentration. Furthermore, the joint inhibition of NH2OH and free nitrous acid led to the substantial accumulation of N2O (up to 32.9 ± 1.3%) during the NO2− reduction phase. In contrast, N2H4 exhibited great potential for facilitating N2O accumulation in both the NO3− reduction and NO2− reduction phases with an accumulation ratio of 13.0 ± 0.3% and 27.5 ± 1.5%, respectively, at the N2H4 concentration of 2.0 mg-N/L. Total enzyme activity analyses revealed that the presence of NH2OH inhibited the activity expression of all denitrifying enzymes, with the downstream enzymes (such as NO2− reductase and N2O reductase) being more susceptible to NH2OH. The addition of N2H4 did not cause a significant decrease in the activity of NO3− reductase and NO2− reductase, but notably curbed the activity of N2O reductase. Based on the responses of the SDAD-related nitrogen metabolic pathway to NH2OH/N2H4, this work provided effective technical means for i) the promotion of NO2− accumulation which might enable the coupling of SDAD with Anammox and ii) the efficient recovery of N2O via the SDAD process.
dc.language	English
dc.publisher	ELSEVIER SCIENCE SA
dc.relation	http://purl.org/au-research/grants/arc/DP220101142
dc.relation.ispartof	Chemical Engineering Journal
dc.relation.isbasedon	10.1016/j.cej.2023.146943
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0904 Chemical Engineering, 0905 Civil Engineering, 0907 Environmental Engineering
dc.subject.classification	Chemical Engineering
dc.subject.classification	4004 Chemical engineering
dc.subject.classification	4011 Environmental engineering
dc.subject.classification	4016 Materials engineering
dc.title	Exposure of sulfur-driven autotrophic denitrification to hydroxylamine/hydrazine: Underlying mechanisms and implications for promoting partial denitrification and N<inf>2</inf>O recovery
dc.type	Journal Article
utslib.citation.volume	477
utslib.for	0904 Chemical Engineering
utslib.for	0905 Civil Engineering
utslib.for	0907 Environmental Engineering
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access	*
dc.date.updated	2024-03-11T01:10:46Z
pubs.publication-status	Published
pubs.volume	477

Abstract:

Hydroxylamine (NH2OH) and hydrazine (N2H4) are crucial intermediates in biological nitrogen removal processes, but their potential effects on the nitrogen-related metabolic pathway during the sulfur-driven autotrophic denitrification (SDAD) process remain unclear and were thus explored in this work. The results of dedicated batch tests indicated that the accumulation ratio of NO2− was positively correlated with the NH2OH concentration, reaching 86.2 ± 2.6% at the NH2OH concentration of 2.0 mg-N/L, while it was barely affected by the N2H4 concentration. Furthermore, the joint inhibition of NH2OH and free nitrous acid led to the substantial accumulation of N2O (up to 32.9 ± 1.3%) during the NO2− reduction phase. In contrast, N2H4 exhibited great potential for facilitating N2O accumulation in both the NO3− reduction and NO2− reduction phases with an accumulation ratio of 13.0 ± 0.3% and 27.5 ± 1.5%, respectively, at the N2H4 concentration of 2.0 mg-N/L. Total enzyme activity analyses revealed that the presence of NH2OH inhibited the activity expression of all denitrifying enzymes, with the downstream enzymes (such as NO2− reductase and N2O reductase) being more susceptible to NH2OH. The addition of N2H4 did not cause a significant decrease in the activity of NO3− reductase and NO2− reductase, but notably curbed the activity of N2O reductase. Based on the responses of the SDAD-related nitrogen metabolic pathway to NH2OH/N2H4, this work provided effective technical means for i) the promotion of NO2− accumulation which might enable the coupling of SDAD with Anammox and ii) the efficient recovery of N2O via the SDAD process.

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