Characterization of the B Cell Receptor Repertoire in the Intestinal Mucosa and of Tumor-Infiltrating Lymphocytes in Colorectal Adenoma and Carcinoma.

Zhang, W; Feng, Q; Wang, C; Zeng, X; Du, Y; Lin, L; Wu, J; Fu, L; Yang, K; Xu, X; Xu, H; Zhao, Y; Li, X; Schoenauer, UH; Stadlmayr, A; Saksena, NK; Tilg, H; Datz, C; Liu, X

Characterization of the B Cell Receptor Repertoire in the Intestinal Mucosa and of Tumor-Infiltrating Lymphocytes in Colorectal Adenoma and Carcinoma.

Zhang, W Feng, Q Wang, C Zeng, X Du, Y Lin, L Wu, J Fu, L Yang, K Xu, X Xu, H

Zhao, Y Li, X Schoenauer, UH Stadlmayr, A Saksena, NK Tilg, H Datz, C Liu, X

Permalink

Publisher:: The American Association of Immunologists
Publication Type:: Journal Article
Citation:: J Immunol, 2017, 198, (9), pp. 3719-3728
Issue Date:: 2017-05-01

Closed Access

	Filename	Description	Size
	Characterization_of_the_B_Cell_Receptor_Repertoire_in_t.pdf	Published version	2.53 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Zhang, W
dc.contributor.author	Feng, Q
dc.contributor.author	Wang, C
dc.contributor.author	Zeng, X
dc.contributor.author	Du, Y
dc.contributor.author	Lin, L
dc.contributor.author	Wu, J
dc.contributor.author	Fu, L
dc.contributor.author	Yang, K
dc.contributor.author	Xu, X
dc.contributor.author	Xu, H https://orcid.org/0000-0003-1129-5337
dc.contributor.author	Zhao, Y
dc.contributor.author	Li, X
dc.contributor.author	Schoenauer, UH
dc.contributor.author	Stadlmayr, A
dc.contributor.author	Saksena, NK
dc.contributor.author	Tilg, H
dc.contributor.author	Datz, C
dc.contributor.author	Liu, X
dc.date.accessioned	2024-03-12T04:02:41Z
dc.date.available	2017-02-23
dc.date.available	2024-03-12T04:02:41Z
dc.date.issued	2017-05-01
dc.identifier.citation	J Immunol, 2017, 198, (9), pp. 3719-3728
dc.identifier.issn	0022-1767
dc.identifier.issn	1550-6606
dc.identifier.uri	http://hdl.handle.net/10453/176553
dc.description.abstract	The B cells inhabited in mucosa play a vital role in mediating homeostasis with autoantigens and external Ags. Tumor-infiltrating lymphocytes are potential prognostic markers and therapeutic agents for cancer. However, the spatial heterogeneity of the B cell repertoire in intestinal mucosa and the tumor-infiltrating lymphocytes in colorectal cancer (CRC) remain poorly understood. In this study, we developed an unbiased method to amplify the IgH repertoire, as well as a bioinformatic pipeline to process these high-throughput sequencing data. With biopsies from seven intestinal mucosal segments, we uncovered their strong spatial homogeneity among the large intestine, where the clone overlap rate was up to 62.21%. The heterogeneity between terminal ileum and large intestine was also observed, including discrepant isotype distribution and low clone overlap rate. With tumor and adjacent normal mucosal tissues from CRC and colorectal advanced adenoma (AD) patients, we observed a similar IgH profile between tumor and adjacent normal mucosal tissues in AD, as well as a slight difference in CRC. Interestingly, we found distinct repertoire properties in the CRC tumor from AD and normal mucosa. Finally, we identified 1445 public clones for the normal mucosa, and 22 public clones for the CRC tumor with characteristic features. These data may be of potential use in clinical prognosis, diagnosis, and treatment of CRC.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	The American Association of Immunologists
dc.relation.ispartof	J Immunol
dc.relation.isbasedon	10.4049/jimmunol.1602039
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	1107 Immunology
dc.subject.classification	Immunology
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3204 Immunology
dc.subject.mesh	Adenoma
dc.subject.mesh	Aged
dc.subject.mesh	Aged, 80 and over
dc.subject.mesh	B-Lymphocytes
dc.subject.mesh	Biopsy
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Colonic Neoplasms
dc.subject.mesh	Colorectal Neoplasms
dc.subject.mesh	Computational Biology
dc.subject.mesh	Genetic Variation
dc.subject.mesh	High-Throughput Nucleotide Sequencing
dc.subject.mesh	Humans
dc.subject.mesh	Immunoglobulin Heavy Chains
dc.subject.mesh	Intestinal Mucosa
dc.subject.mesh	Lymphocytes, Tumor-Infiltrating
dc.subject.mesh	Middle Aged
dc.subject.mesh	Neoplasm Staging
dc.subject.mesh	Receptors, Antigen, B-Cell
dc.subject.mesh	Intestinal Mucosa
dc.subject.mesh	B-Lymphocytes
dc.subject.mesh	Lymphocytes, Tumor-Infiltrating
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Humans
dc.subject.mesh	Adenoma
dc.subject.mesh	Colorectal Neoplasms
dc.subject.mesh	Colonic Neoplasms
dc.subject.mesh	Receptors, Antigen, B-Cell
dc.subject.mesh	Biopsy
dc.subject.mesh	Neoplasm Staging
dc.subject.mesh	Computational Biology
dc.subject.mesh	Aged
dc.subject.mesh	Aged, 80 and over
dc.subject.mesh	Middle Aged
dc.subject.mesh	Immunoglobulin Heavy Chains
dc.subject.mesh	Genetic Variation
dc.subject.mesh	High-Throughput Nucleotide Sequencing
dc.subject.mesh	Adenoma
dc.subject.mesh	Aged
dc.subject.mesh	Aged, 80 and over
dc.subject.mesh	B-Lymphocytes
dc.subject.mesh	Biopsy
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Colonic Neoplasms
dc.subject.mesh	Colorectal Neoplasms
dc.subject.mesh	Computational Biology
dc.subject.mesh	Genetic Variation
dc.subject.mesh	High-Throughput Nucleotide Sequencing
dc.subject.mesh	Humans
dc.subject.mesh	Immunoglobulin Heavy Chains
dc.subject.mesh	Intestinal Mucosa
dc.subject.mesh	Lymphocytes, Tumor-Infiltrating
dc.subject.mesh	Middle Aged
dc.subject.mesh	Neoplasm Staging
dc.subject.mesh	Receptors, Antigen, B-Cell
dc.title	Characterization of the B Cell Receptor Repertoire in the Intestinal Mucosa and of Tumor-Infiltrating Lymphocytes in Colorectal Adenoma and Carcinoma.
dc.type	Journal Article
utslib.citation.volume	198
utslib.location.activity	United States
utslib.for	1107 Immunology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Computer Science
utslib.copyright.status	closed_access	*
dc.date.updated	2024-03-12T04:02:39Z
pubs.issue	9
pubs.publication-status	Published
pubs.volume	198
utslib.citation.issue	9

Abstract:

The B cells inhabited in mucosa play a vital role in mediating homeostasis with autoantigens and external Ags. Tumor-infiltrating lymphocytes are potential prognostic markers and therapeutic agents for cancer. However, the spatial heterogeneity of the B cell repertoire in intestinal mucosa and the tumor-infiltrating lymphocytes in colorectal cancer (CRC) remain poorly understood. In this study, we developed an unbiased method to amplify the IgH repertoire, as well as a bioinformatic pipeline to process these high-throughput sequencing data. With biopsies from seven intestinal mucosal segments, we uncovered their strong spatial homogeneity among the large intestine, where the clone overlap rate was up to 62.21%. The heterogeneity between terminal ileum and large intestine was also observed, including discrepant isotype distribution and low clone overlap rate. With tumor and adjacent normal mucosal tissues from CRC and colorectal advanced adenoma (AD) patients, we observed a similar IgH profile between tumor and adjacent normal mucosal tissues in AD, as well as a slight difference in CRC. Interestingly, we found distinct repertoire properties in the CRC tumor from AD and normal mucosa. Finally, we identified 1445 public clones for the normal mucosa, and 22 public clones for the CRC tumor with characteristic features. These data may be of potential use in clinical prognosis, diagnosis, and treatment of CRC.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/176553