Converting food waste into high-value medium chain fatty acids and long chain alcohols via chain elongation with an internally produced electron donor

Wu, L; Wei, W; Qian, J; Chen, X; Ni, BJ

Converting food waste into high-value medium chain fatty acids and long chain alcohols via chain elongation with an internally produced electron donor

Wu, L

Wei, W

Qian, J Chen, X Ni, BJ

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Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: Green Chemistry, 2023, 25, (24), pp. 10567-10575
Issue Date:: 2023-11-10

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Field	Value	Language
dc.contributor.author	Wu, L https://orcid.org/0000-0001-8581-2937
dc.contributor.author	Wei, W https://orcid.org/0000-0001-5613-337X
dc.contributor.author	Qian, J
dc.contributor.author	Chen, X
dc.contributor.author	Ni, BJ
dc.date.accessioned	2024-05-20T03:33:41Z
dc.date.available	2024-05-20T03:33:41Z
dc.date.issued	2023-11-10
dc.identifier.citation	Green Chemistry, 2023, 25, (24), pp. 10567-10575
dc.identifier.issn	1463-9262
dc.identifier.issn	1463-9270
dc.identifier.uri	http://hdl.handle.net/10453/179051
dc.description.abstract	To improve the economic gain of anaerobic fermentation, this study proposes a novel chain elongation-based biotechnology of converting food waste (FW) into medium chain fatty acids (MCFAs) and long chain alcohols (LCA). These two fermentative products are more profitable due to their higher energy density and easier separation compared to short-chain fatty acids (SCFAs). The necessity and the cost for externally dosing an electron donor (ED) for chain elongation were eliminated by inoculating different yeast levels (2.25 × 107, 3.37 × 107, 6.74 × 107, and 8.99 × 107 cells per FW ml Saccharomyces cerevisiae) into the fermenters. In situ ethanol production was then facilitated and was subsequently utilized to produce MCFAs. Overall, MCFA formation was enhanced by yeast inoculation compared to the control. 533 mg of COD per L MCFAs were generated in the batch test with 2.25 × 107 cells per FW ml S. cerevisiae. Higher but similar MCFA yields (∼1500 mg COD per L) were obtained in batch reactors with higher yeast inoculations. The greater endogenous ethanol and SCFA supply, enriched chain elongating organisms, along with the promoted CE pathway based on the increasing gene abundance jointly contributed to the higher MCFA production from FW with yeast inoculation. More interestingly, a high accumulative butanol level (1228 mg COD per L) was observed under 8.99 × 107 cells per FW ml S. cerevisiae inoculation. The more abundant genes encoding the acetone-butanol-ethanol platform and the lower conversion from butanol to caproate likely induced such a high butanol yield according to metagenomics analysis.
dc.language	English
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation	http://purl.org/au-research/grants/arc/DP220101139
dc.relation	http://purl.org/au-research/grants/arc/DE220100530
dc.relation.ispartof	Green Chemistry
dc.relation.isbasedon	10.1039/d3gc03614f
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences
dc.subject.classification	Organic Chemistry
dc.subject.classification	34 Chemical sciences
dc.title	Converting food waste into high-value medium chain fatty acids and long chain alcohols via chain elongation with an internally produced electron donor
dc.type	Journal Article
utslib.citation.volume	25
utslib.for	03 Chemical Sciences
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2024-05-20T03:33:39Z
pubs.issue	24
pubs.publication-status	Published
pubs.volume	25
utslib.citation.issue	24

Abstract:

To improve the economic gain of anaerobic fermentation, this study proposes a novel chain elongation-based biotechnology of converting food waste (FW) into medium chain fatty acids (MCFAs) and long chain alcohols (LCA). These two fermentative products are more profitable due to their higher energy density and easier separation compared to short-chain fatty acids (SCFAs). The necessity and the cost for externally dosing an electron donor (ED) for chain elongation were eliminated by inoculating different yeast levels (2.25 × 107, 3.37 × 107, 6.74 × 107, and 8.99 × 107 cells per FW ml Saccharomyces cerevisiae) into the fermenters. In situ ethanol production was then facilitated and was subsequently utilized to produce MCFAs. Overall, MCFA formation was enhanced by yeast inoculation compared to the control. 533 mg of COD per L MCFAs were generated in the batch test with 2.25 × 107 cells per FW ml S. cerevisiae. Higher but similar MCFA yields (∼1500 mg COD per L) were obtained in batch reactors with higher yeast inoculations. The greater endogenous ethanol and SCFA supply, enriched chain elongating organisms, along with the promoted CE pathway based on the increasing gene abundance jointly contributed to the higher MCFA production from FW with yeast inoculation. More interestingly, a high accumulative butanol level (1228 mg COD per L) was observed under 8.99 × 107 cells per FW ml S. cerevisiae inoculation. The more abundant genes encoding the acetone-butanol-ethanol platform and the lower conversion from butanol to caproate likely induced such a high butanol yield according to metagenomics analysis.

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http://hdl.handle.net/10453/179051