Morphology engineering of nanofibrous poly(acrylonitrile)-based strong anion exchange membranes for enhanced protein adsorption and recovery

AL-Attabi, R; Merenda, A; Hsia, T; Sriramoju, B; Dumée, LF; Thang, SH; Pham, H; Yang, X; Kong, L

Morphology engineering of nanofibrous poly(acrylonitrile)-based strong anion exchange membranes for enhanced protein adsorption and recovery

AL-Attabi, R Merenda, A

Hsia, T Sriramoju, B Dumée, LF Thang, SH Pham, H Yang, X Kong, L

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Journal of Water Process Engineering, 2024, 65, pp. 105750
Issue Date:: 2024-08-01

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Field	Value	Language
dc.contributor.author	AL-Attabi, R
dc.contributor.author	Merenda, A https://orcid.org/0000-0002-9998-0330
dc.contributor.author	Hsia, T
dc.contributor.author	Sriramoju, B
dc.contributor.author	Dumée, LF
dc.contributor.author	Thang, SH
dc.contributor.author	Pham, H
dc.contributor.author	Yang, X
dc.contributor.author	Kong, L
dc.date.accessioned	2024-10-09T03:04:14Z
dc.date.available	2024-10-09T03:04:14Z
dc.date.issued	2024-08-01
dc.identifier.citation	Journal of Water Process Engineering, 2024, 65, pp. 105750
dc.identifier.issn	2214-7144
dc.identifier.uri	http://hdl.handle.net/10453/181305
dc.description.abstract	The (bio)pharmaceutical and food industries are transitioning towards circular economy to minimise waste and foster sustainability in the supply chain of biomolecule-based drugs and supplements. Membrane chromatography is expected to play a crucial role in this transition, with the potential to maximize recovery and reduce operating costs. The development of chromatographical membranes is however impeded by the lack of understanding on the membrane structure – chemistry – performance relationship. Here a one-pot synthesis of anion exchange membranes with engineered nanofibrous morphology was studied by electrospinning the blend of polyacrylonitrile (PAN) and tailored quaternized poly(acrylonitrile)-co-poly(2-(dimethylamino)ethyl acrylate) copolymer containing 15 mol% of functional groups, namely PAN-pAQ membranes. Comprehensive evaluations were conducted on the influence of the electrospinning parameters on the nanofibre morphology, surface properties and binding capacity of model protein bovine serum albumin (BSA). The fibre alignment was shown to play a significant role, i.e., membrane with anisotropic fibres obtained by increasing the drum rotational speed to 2000 rpm allowed a high BSA binding capacity of 166 mg∙g−1, i.e., 45–66 % higher than other membranes with randomly-oriented nanofibres. This study is expected to contribute towards the design of future membranes for effective purification and recovery of biomolecules from natural and waste streams, promoting circular bio-economy.
dc.language	en
dc.publisher	Elsevier
dc.relation	http://purl.org/au-research/grants/arc/DP230100238
dc.relation.ispartof	Journal of Water Process Engineering
dc.relation.isbasedon	10.1016/j.jwpe.2024.105750
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0905 Civil Engineering, 0907 Environmental Engineering
dc.subject.classification	4004 Chemical engineering
dc.subject.classification	4005 Civil engineering
dc.subject.classification	4011 Environmental engineering
dc.title	Morphology engineering of nanofibrous poly(acrylonitrile)-based strong anion exchange membranes for enhanced protein adsorption and recovery
dc.type	Journal Article
utslib.citation.volume	65
utslib.for	0905 Civil Engineering
utslib.for	0907 Environmental Engineering
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	recently_added	*
dc.date.updated	2024-10-09T03:04:11Z
pubs.publication-status	Published
pubs.volume	65

Abstract:

The (bio)pharmaceutical and food industries are transitioning towards circular economy to minimise waste and foster sustainability in the supply chain of biomolecule-based drugs and supplements. Membrane chromatography is expected to play a crucial role in this transition, with the potential to maximize recovery and reduce operating costs. The development of chromatographical membranes is however impeded by the lack of understanding on the membrane structure – chemistry – performance relationship. Here a one-pot synthesis of anion exchange membranes with engineered nanofibrous morphology was studied by electrospinning the blend of polyacrylonitrile (PAN) and tailored quaternized poly(acrylonitrile)-co-poly(2-(dimethylamino)ethyl acrylate) copolymer containing 15 mol% of functional groups, namely PAN-pAQ membranes. Comprehensive evaluations were conducted on the influence of the electrospinning parameters on the nanofibre morphology, surface properties and binding capacity of model protein bovine serum albumin (BSA). The fibre alignment was shown to play a significant role, i.e., membrane with anisotropic fibres obtained by increasing the drum rotational speed to 2000 rpm allowed a high BSA binding capacity of 166 mg∙g−1, i.e., 45–66 % higher than other membranes with randomly-oriented nanofibres. This study is expected to contribute towards the design of future membranes for effective purification and recovery of biomolecules from natural and waste streams, promoting circular bio-economy.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/181305