Molecular characterization and human health risk assessment of multi-drug and heavy metals tolerant bacteria from urban river water

Kabir, MM; Maleha, SM; Hossain, MS; Sultana, N; Islam, R; Islam, S; Ahmed, F; Bahadur, NM; Choudhury, TR; Didar-ul-Alam, M; Kabir, N; Tijing, L; Shon, HK

Molecular characterization and human health risk assessment of multi-drug and heavy metals tolerant bacteria from urban river water

Kabir, MM Maleha, SM Hossain, MS Sultana, N Islam, R Islam, S Ahmed, F Bahadur, NM Choudhury, TR Didar-ul-Alam, M Kabir, N Tijing, L

Shon, HK

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Publisher:: ELSEVIER SCIENCE INC
Publication Type:: Journal Article
Citation:: Desalination and Water Treatment, 2024, 317
Issue Date:: 2024-01-01

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Field	Value	Language
dc.contributor.author	Kabir, MM
dc.contributor.author	Maleha, SM
dc.contributor.author	Hossain, MS
dc.contributor.author	Sultana, N
dc.contributor.author	Islam, R
dc.contributor.author	Islam, S
dc.contributor.author	Ahmed, F
dc.contributor.author	Bahadur, NM
dc.contributor.author	Choudhury, TR
dc.contributor.author	Didar-ul-Alam, M
dc.contributor.author	Kabir, N
dc.contributor.author	Tijing, L https://orcid.org/0000-0001-9398-6355
dc.contributor.author	Shon, HK
dc.date.accessioned	2025-03-17T01:48:52Z
dc.date.available	2025-03-17T01:48:52Z
dc.date.issued	2024-01-01
dc.identifier.citation	Desalination and Water Treatment, 2024, 317
dc.identifier.issn	1944-3994
dc.identifier.issn	1944-3986
dc.identifier.uri	http://hdl.handle.net/10453/185863
dc.description.abstract	The present study characterized the multi-drug and heavy metal-resistant bacteria and their human health risks in the six major urban rivers. The bacterial strains were identified by molecular techniques based on 16 s rDNA gene sequence analysis, where most of the isolates belong to Bacillus spp. and Staphylococcus spp. The minimum inhibitory concentration (MIC) of the bacterial strains to different heavy metals like chromium, lead, cadmium, cobalt, mercury, and nickel ranged up to 3000 mg/L. The antibiotic susceptibility tests revealed that 69.23% of the strains resistant to ceftriaxone, while 61.54% were resilient to cefotaxime, 53.85% to ampicillin, 46.15% to amoxicillin, 30.77% to streptomycin, 15.38% to azithromycin, 15.38% to chloramphenicol, 7.69% to tetracycline, 7.69% to gentamycin, 7.69% to vancomycin. Interestingly, ciprofloxacin was found highly sensitive to all the bacterial strains in the present study. The multiple heavy metals resistance (MHMR) index of all the bacterial strains in the present study was very high compared to the standard value of 0.50. The multiple antibiotics resistance (MAR) index was highest for Bacillus cereus MKSMPbT1 (0.45), while it was lowest in B. xiamenesis MKSMCrB1 and B. pumilus MKSMNiT1 (0.09). The results of the hemolytic assay revealed that almost all the bacterial strains identified in the present study are highly pathogenic in nature. In essence, the bacterial strains identified in the present study could pose significant environmental and public health concerns that draw strict government attention.
dc.language	English
dc.publisher	ELSEVIER SCIENCE INC
dc.relation	University of Technology SydneyUTSCPDRF06
dc.relation.ispartof	Desalination and Water Treatment
dc.relation.isbasedon	10.1016/j.dwt.2024.100298
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0904 Chemical Engineering, 0905 Civil Engineering, 0907 Environmental Engineering
dc.subject.classification	4004 Chemical engineering
dc.subject.classification	4005 Civil engineering
dc.subject.classification	4011 Environmental engineering
dc.title	Molecular characterization and human health risk assessment of multi-drug and heavy metals tolerant bacteria from urban river water
dc.type	Journal Article
utslib.citation.volume	317
utslib.for	0904 Chemical Engineering
utslib.for	0905 Civil Engineering
utslib.for	0907 Environmental Engineering
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Technology in Water and Wastewater (CTWW)
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-03-17T01:48:49Z
pubs.publication-status	Published
pubs.volume	317

Abstract:

The present study characterized the multi-drug and heavy metal-resistant bacteria and their human health risks in the six major urban rivers. The bacterial strains were identified by molecular techniques based on 16 s rDNA gene sequence analysis, where most of the isolates belong to Bacillus spp. and Staphylococcus spp. The minimum inhibitory concentration (MIC) of the bacterial strains to different heavy metals like chromium, lead, cadmium, cobalt, mercury, and nickel ranged up to 3000 mg/L. The antibiotic susceptibility tests revealed that 69.23% of the strains resistant to ceftriaxone, while 61.54% were resilient to cefotaxime, 53.85% to ampicillin, 46.15% to amoxicillin, 30.77% to streptomycin, 15.38% to azithromycin, 15.38% to chloramphenicol, 7.69% to tetracycline, 7.69% to gentamycin, 7.69% to vancomycin. Interestingly, ciprofloxacin was found highly sensitive to all the bacterial strains in the present study. The multiple heavy metals resistance (MHMR) index of all the bacterial strains in the present study was very high compared to the standard value of 0.50. The multiple antibiotics resistance (MAR) index was highest for Bacillus cereus MKSMPbT1 (0.45), while it was lowest in B. xiamenesis MKSMCrB1 and B. pumilus MKSMNiT1 (0.09). The results of the hemolytic assay revealed that almost all the bacterial strains identified in the present study are highly pathogenic in nature. In essence, the bacterial strains identified in the present study could pose significant environmental and public health concerns that draw strict government attention.

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http://hdl.handle.net/10453/185863