Competitive enrichment of comammox Nitrospira in floccular sludge.

Hou, J; Zhu, Y; Liu, J; Lin, L; Zheng, M; Yang, L; Wei, W; Ni, B-J; Chen, X

Competitive enrichment of comammox Nitrospira in floccular sludge.

Hou, J Zhu, Y Liu, J Lin, L Zheng, M Yang, L Wei, W

Ni, B-J Chen, X

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Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Water Res, 2024, 251, pp. 121151
Issue Date:: 2024-03-01

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Field	Value	Language
dc.contributor.author	Hou, J
dc.contributor.author	Zhu, Y
dc.contributor.author	Liu, J
dc.contributor.author	Lin, L
dc.contributor.author	Zheng, M
dc.contributor.author	Yang, L
dc.contributor.author	Wei, W https://orcid.org/0000-0001-5613-337X
dc.contributor.author	Ni, B-J
dc.contributor.author	Chen, X
dc.date.accessioned	2025-03-21T09:58:40Z
dc.date.available	2024-01-15
dc.date.available	2025-03-21T09:58:40Z
dc.date.issued	2024-03-01
dc.identifier.citation	Water Res, 2024, 251, pp. 121151
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/186109
dc.description.abstract	The discovery of complete ammonium oxidation (comammox) has subverted the traditional perception of two-step nitrification, which plays a key role in achieving biological nitrogen removal from wastewater. Floccular sludge-based treatment technologies are being applied at the majority of wastewater treatment plants in service where detection of various abundances and activities of comammox bacteria have been reported. However, limited efforts have been made to enrich and subsequently characterize comammox bacteria in floccular sludge. To this end, a lab-scale sequencing batch reactor (SBR) in the step-feeding mode was applied in this work to enrich comammox bacteria through controlling appropriate operational conditions (dissolved oxygen of 0.5 ± 0.1 g-O2/m3, influent ammonium of 40 g-N/m3 and uncontrolled longer sludge retention time). After 215-d operation, comammox bacteria gradually gained competitive advantages over counterparts in the SBR with a stable nitrification efficiency of 92.2 ± 2.2 %: the relative abundance of Nitrospira reached 42.9 ± 1.3 %, which was 13 times higher than that of Nitrosomonas, and the amoA gene level of comammox bacteria increased to 7.7 ± 2.1 × 106 copies/g-biomass, nearly 50 times higher than that of conventional ammonium-oxidizing bacteria. The enrichment of comammox bacteria, especially Clade A Candidatus Nitrospira nitrosa, in the floccular sludge led to (i) apparent affinity constants for ammonium and oxygen of 3.296 ± 0.989 g-N/m3 and 0.110 ± 0.004 g-O2/m3, respectively, and (ii) significantly low N2O and NO production, with emission factors being 0.136 ± 0.026 % and 0.023 ± 0.013 %, respectively.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation	http://purl.org/au-research/grants/arc/DP220101142
dc.relation.ispartof	Water Res
dc.relation.isbasedon	10.1016/j.watres.2024.121151
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	Sewage
dc.subject.mesh	Ammonia
dc.subject.mesh	Bacteria
dc.subject.mesh	Nitrification
dc.subject.mesh	Ammonium Compounds
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Oxygen
dc.subject.mesh	Phylogeny
dc.subject.mesh	Archaea
dc.subject.mesh	Bacteria
dc.subject.mesh	Archaea
dc.subject.mesh	Oxygen
dc.subject.mesh	Ammonia
dc.subject.mesh	Ammonium Compounds
dc.subject.mesh	Sewage
dc.subject.mesh	Phylogeny
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Nitrification
dc.subject.mesh	Sewage
dc.subject.mesh	Ammonia
dc.subject.mesh	Bacteria
dc.subject.mesh	Nitrification
dc.subject.mesh	Ammonium Compounds
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Oxygen
dc.subject.mesh	Phylogeny
dc.subject.mesh	Archaea
dc.title	Competitive enrichment of comammox Nitrospira in floccular sludge.
dc.type	Journal Article
utslib.citation.volume	251
utslib.location.activity	England
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Technology in Water and Wastewater (CTWW)
utslib.copyright.status	recently_added	*
dc.date.updated	2025-03-21T09:58:37Z
pubs.publication-status	Published
pubs.volume	251

Abstract:

The discovery of complete ammonium oxidation (comammox) has subverted the traditional perception of two-step nitrification, which plays a key role in achieving biological nitrogen removal from wastewater. Floccular sludge-based treatment technologies are being applied at the majority of wastewater treatment plants in service where detection of various abundances and activities of comammox bacteria have been reported. However, limited efforts have been made to enrich and subsequently characterize comammox bacteria in floccular sludge. To this end, a lab-scale sequencing batch reactor (SBR) in the step-feeding mode was applied in this work to enrich comammox bacteria through controlling appropriate operational conditions (dissolved oxygen of 0.5 ± 0.1 g-O2/m3, influent ammonium of 40 g-N/m3 and uncontrolled longer sludge retention time). After 215-d operation, comammox bacteria gradually gained competitive advantages over counterparts in the SBR with a stable nitrification efficiency of 92.2 ± 2.2 %: the relative abundance of Nitrospira reached 42.9 ± 1.3 %, which was 13 times higher than that of Nitrosomonas, and the amoA gene level of comammox bacteria increased to 7.7 ± 2.1 × 106 copies/g-biomass, nearly 50 times higher than that of conventional ammonium-oxidizing bacteria. The enrichment of comammox bacteria, especially Clade A Candidatus Nitrospira nitrosa, in the floccular sludge led to (i) apparent affinity constants for ammonium and oxygen of 3.296 ± 0.989 g-N/m3 and 0.110 ± 0.004 g-O2/m3, respectively, and (ii) significantly low N2O and NO production, with emission factors being 0.136 ± 0.026 % and 0.023 ± 0.013 %, respectively.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/186109