Novel multiplexed alkali enzyme lysis coupled with EDTA pretreatment for RNA virus extraction from wastewater sludge: Optimization, recovery, and detection.

Dong, T; Ai, J; Zong, Y; Zhang, Y; Li, L; Zhou, H; Peng, S; He, H; Zhang, Z; Wang, Q

Novel multiplexed alkali enzyme lysis coupled with EDTA pretreatment for RNA virus extraction from wastewater sludge: Optimization, recovery, and detection.

Dong, T Ai, J Zong, Y Zhang, Y Li, L Zhou, H Peng, S He, H Zhang, Z Wang, Q

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Publisher:: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: J Environ Manage, 2024, 352, pp. 120102
Issue Date:: 2024-02-14

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Field	Value	Language
dc.contributor.author	Dong, T
dc.contributor.author	Ai, J
dc.contributor.author	Zong, Y
dc.contributor.author	Zhang, Y
dc.contributor.author	Li, L
dc.contributor.author	Zhou, H
dc.contributor.author	Peng, S
dc.contributor.author	He, H
dc.contributor.author	Zhang, Z
dc.contributor.author	Wang, Q https://orcid.org/0000-0002-5744-2331
dc.date.accessioned	2025-03-21T10:22:13Z
dc.date.available	2024-01-11
dc.date.available	2025-03-21T10:22:13Z
dc.date.issued	2024-02-14
dc.identifier.citation	J Environ Manage, 2024, 352, pp. 120102
dc.identifier.issn	0301-4797
dc.identifier.issn	1095-8630
dc.identifier.uri	http://hdl.handle.net/10453/186113
dc.description.abstract	RNA viruses are readily enriched in wastewater sludge owing to adsorption by extracellular polymeric substances (EPS) during wastewater treatment, causing pathogenicity. However, conventional wastewater extraction methods often fail to fully extract these viruses from sludge. In this study, three methods: enzymatic (ENP), alkaline (ALP), and ethylenediaminetetraacetic acid (EDTA) pretreatments were applied to sludges and promote the RNA virus extraction from sludge. Our results show that the total recovery rate of RNA viruses increased by 87.73% after ENP pretreatment, whereas ALP pretreatment inhibited virus extraction. The highest recovery rate of viruses from sludge, reaching 296.80%, was achieved with EDTA pretreatment (EDP) coupled with ENP. Notably, the most significant increase was observed in the abundance of Astroviruses, which increased from 7.60 × 107 to 7.86 × 108 copies/g TSS after EDP + ENP treatment. Our investigations revealed that virus extraction was affected by a class of short-wavelength protein substances, as opposed to tryptophan or tyrosine, which were eluted by proteins with beef paste buffer by substitution after EDP + ENP treatment. The results of this study provide essential insights for sludge-based epidemiology with the required sensitivity for managing the extraction of RNA epidemic viruses to control viral transmission.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
dc.relation.ispartof	J Environ Manage
dc.relation.isbasedon	10.1016/j.jenvman.2024.120102
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Sciences
dc.subject.mesh	Animals
dc.subject.mesh	Cattle
dc.subject.mesh	Wastewater
dc.subject.mesh	Sewage
dc.subject.mesh	Edetic Acid
dc.subject.mesh	Viruses
dc.subject.mesh	RNA Viruses
dc.subject.mesh	Proteins
dc.subject.mesh	Animals
dc.subject.mesh	Cattle
dc.subject.mesh	Viruses
dc.subject.mesh	RNA Viruses
dc.subject.mesh	Edetic Acid
dc.subject.mesh	Proteins
dc.subject.mesh	Sewage
dc.subject.mesh	Wastewater
dc.subject.mesh	Animals
dc.subject.mesh	Cattle
dc.subject.mesh	Wastewater
dc.subject.mesh	Sewage
dc.subject.mesh	Edetic Acid
dc.subject.mesh	Viruses
dc.subject.mesh	RNA Viruses
dc.subject.mesh	Proteins
dc.title	Novel multiplexed alkali enzyme lysis coupled with EDTA pretreatment for RNA virus extraction from wastewater sludge: Optimization, recovery, and detection.
dc.type	Journal Article
utslib.citation.volume	352
utslib.location.activity	England
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Technology in Water and Wastewater (CTWW)
utslib.copyright.status	closed_access	*
dc.date.updated	2025-03-21T10:22:08Z
pubs.publication-status	Published
pubs.volume	352

Abstract:

RNA viruses are readily enriched in wastewater sludge owing to adsorption by extracellular polymeric substances (EPS) during wastewater treatment, causing pathogenicity. However, conventional wastewater extraction methods often fail to fully extract these viruses from sludge. In this study, three methods: enzymatic (ENP), alkaline (ALP), and ethylenediaminetetraacetic acid (EDTA) pretreatments were applied to sludges and promote the RNA virus extraction from sludge. Our results show that the total recovery rate of RNA viruses increased by 87.73% after ENP pretreatment, whereas ALP pretreatment inhibited virus extraction. The highest recovery rate of viruses from sludge, reaching 296.80%, was achieved with EDTA pretreatment (EDP) coupled with ENP. Notably, the most significant increase was observed in the abundance of Astroviruses, which increased from 7.60 × 107 to 7.86 × 108 copies/g TSS after EDP + ENP treatment. Our investigations revealed that virus extraction was affected by a class of short-wavelength protein substances, as opposed to tryptophan or tyrosine, which were eluted by proteins with beef paste buffer by substitution after EDP + ENP treatment. The results of this study provide essential insights for sludge-based epidemiology with the required sensitivity for managing the extraction of RNA epidemic viruses to control viral transmission.

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http://hdl.handle.net/10453/186113