Novel agents consisting of Pseudomonas zhaodongensis and dimethylsulfoniopropionate (DMSP) enhancing bioremediation of oil-contaminated sediments at deep-sea condition

Qiao, Y; Xu, W; Wei, J; Kong, L; Xue, J; Jiang, Q; Cheng, D; Liu, Y

Novel agents consisting of Pseudomonas zhaodongensis and dimethylsulfoniopropionate (DMSP) enhancing bioremediation of oil-contaminated sediments at deep-sea condition

Qiao, Y Xu, W Wei, J Kong, L Xue, J Jiang, Q Cheng, D Liu, Y

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Environmental Technology and Innovation, 2024, 36, pp. 103744
Issue Date:: 2024-11-01

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Field	Value	Language
dc.contributor.author	Qiao, Y
dc.contributor.author	Xu, W
dc.contributor.author	Wei, J
dc.contributor.author	Kong, L
dc.contributor.author	Xue, J
dc.contributor.author	Jiang, Q
dc.contributor.author	Cheng, D
dc.contributor.author	Liu, Y
dc.date.accessioned	2025-03-25T02:30:45Z
dc.date.available	2025-03-25T02:30:45Z
dc.date.issued	2024-11-01
dc.identifier.citation	Environmental Technology and Innovation, 2024, 36, pp. 103744
dc.identifier.issn	2352-1864
dc.identifier.issn	2352-1864
dc.identifier.uri	http://hdl.handle.net/10453/186189
dc.description.abstract	Deep-sea environments are featured by low temperature and high hydrostatic pressure, which inhibits petroleum hydrocarbon metabolism by microorganisms. Herein, we developed novel bioremediating agents composed of different combinations of Pseudomonas zhaodongensis and dimethylsulfoniopropionate (DMSP) to promote oil degradation at deep-sea microcosm environment. First, through transcriptome sequencing, we revealed DMSP might provide hydrostatic pressure protection via secretion of potential piezolytes within the cell, which let bacteria healthy growth and thereby promoted oil biodegradation. Then, via oil measurement and high-throughput sequencing, we assessed effectiveness on using the studied agents and indigenous microorganism (i.e., natural remediation) to restore oil-contaminated muddy and sandy sediments at the microcosm, and demonstrated: 1) Oil degradation efficiency among different treatments using agents was 23.47 % – 41.02 % higher than that in natural remediation; 2) Each remediation plan defined specialized bacterial community. Marinobacter, Idiomarina, Sulfitobacter, Ferrimonas, Halodesulfovibrio, Paramaledivibacter and Pseudomonas were keystone oil-degrading taxa; 3) Overall, microbial community in sediment samples treated by bioremediation agents obtained better diversification of trophic interactions, structure stability and interference resistance; 4) Compared to natural remediation, pathways involving in oil component degradation and biogeochemical cycling exhibited varying degrees of up-regulation in agent-treated groups. Altogether, these results emphasize the crucial role of P. zhaodongensis and DMSP in enhancing bioremediation of oil-polluted sediments at typical deep-sea condition, and provide a novel idea for in-situ restoration of oil pollution at deep sea in future.
dc.language	en
dc.publisher	Elsevier
dc.relation.ispartof	Environmental Technology and Innovation
dc.relation.isbasedon	10.1016/j.eti.2024.103744
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0502 Environmental Science and Management, 0907 Environmental Engineering, 1002 Environmental Biotechnology
dc.title	Novel agents consisting of Pseudomonas zhaodongensis and dimethylsulfoniopropionate (DMSP) enhancing bioremediation of oil-contaminated sediments at deep-sea condition
dc.type	Journal Article
utslib.citation.volume	36
utslib.for	0502 Environmental Science and Management
utslib.for	0907 Environmental Engineering
utslib.for	1002 Environmental Biotechnology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-03-25T02:30:43Z
pubs.publication-status	Published
pubs.volume	36

Abstract:

Deep-sea environments are featured by low temperature and high hydrostatic pressure, which inhibits petroleum hydrocarbon metabolism by microorganisms. Herein, we developed novel bioremediating agents composed of different combinations of Pseudomonas zhaodongensis and dimethylsulfoniopropionate (DMSP) to promote oil degradation at deep-sea microcosm environment. First, through transcriptome sequencing, we revealed DMSP might provide hydrostatic pressure protection via secretion of potential piezolytes within the cell, which let bacteria healthy growth and thereby promoted oil biodegradation. Then, via oil measurement and high-throughput sequencing, we assessed effectiveness on using the studied agents and indigenous microorganism (i.e., natural remediation) to restore oil-contaminated muddy and sandy sediments at the microcosm, and demonstrated: 1) Oil degradation efficiency among different treatments using agents was 23.47 % – 41.02 % higher than that in natural remediation; 2) Each remediation plan defined specialized bacterial community. Marinobacter, Idiomarina, Sulfitobacter, Ferrimonas, Halodesulfovibrio, Paramaledivibacter and Pseudomonas were keystone oil-degrading taxa; 3) Overall, microbial community in sediment samples treated by bioremediation agents obtained better diversification of trophic interactions, structure stability and interference resistance; 4) Compared to natural remediation, pathways involving in oil component degradation and biogeochemical cycling exhibited varying degrees of up-regulation in agent-treated groups. Altogether, these results emphasize the crucial role of P. zhaodongensis and DMSP in enhancing bioremediation of oil-polluted sediments at typical deep-sea condition, and provide a novel idea for in-situ restoration of oil pollution at deep sea in future.

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