Inhibition of norfloxacin on fermentative hydrogen production: Performance evaluation and metagenomic analysis

Gao, T; Sun, D; Sun, G; Xue, S; Chen, Y; Zhou, Y; Wong, JWC; Yang, G; Zhang, G; Ngo, HH

Inhibition of norfloxacin on fermentative hydrogen production: Performance evaluation and metagenomic analysis

Gao, T Sun, D Sun, G Xue, S Chen, Y

Zhou, Y Wong, JWC Yang, G Zhang, G Ngo, HH

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Publisher:: ELSEVIER SCIENCE SA
Publication Type:: Journal Article
Citation:: Chemical Engineering Journal, 2024, 486
Issue Date:: 2024-04-15

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Field	Value	Language
dc.contributor.author	Gao, T
dc.contributor.author	Sun, D
dc.contributor.author	Sun, G
dc.contributor.author	Xue, S
dc.contributor.author	Chen, Y https://orcid.org/0000-0002-5175-6997
dc.contributor.author	Zhou, Y
dc.contributor.author	Wong, JWC
dc.contributor.author	Yang, G
dc.contributor.author	Zhang, G
dc.contributor.author	Ngo, HH
dc.date.accessioned	2025-04-02T04:32:00Z
dc.date.available	2025-04-02T04:32:00Z
dc.date.issued	2024-04-15
dc.identifier.citation	Chemical Engineering Journal, 2024, 486
dc.identifier.issn	1385-8947
dc.identifier.issn	1873-3212
dc.identifier.uri	http://hdl.handle.net/10453/186464
dc.description.abstract	Toxic chemicals can inhibit the dark fermentation process for renewable biohydrogen production. Norfloxacin (NOR), a typical antibiotic, is present in various feedstocks of dark fermentation, which has excellent antibacterial properties. It is hypothesized that NOR could cause adverse effects on fermentative hydrogen production, while current understanding of this subject is scare. This study investigated the potential inhibitory effect of NOR on dark hydrogen fermentation, and explored the underlying mechanisms from the perspectives of bacterial community structure and functional gene abundance. The results showed that NOR significantly reduced the hydrogen-producing capacity upon exposure to concentrations exceeding 10 mg/L. The hydrogen yield decreased from 1.56 to 0.27 H2/mol-glucose at NOR concentrations of 50 mg/L. NOR also prolonged the hydrogen-producing lag period. Bacterial community analysis revealed that NOR reduced the abundance of high-yielding H2-generating bacteria, such as Clostridium sp., while increasing the abundance of H2-consuming bacteria (e.g. Enterococcus sp.). Metagenomic analysis further indicated that crucial genes involved in glycolysis (e.g. HK, tal-pgi, pfk and PK) and hydrogen formation (e.g. por and E1.12.7.2) were remarkably downregulated under NOR exposure, essentially causing the inhibition of hydrogen production. This study contributes to a better understanding of how antibiotics inhibit dark hydrogen fermentation and provides a theoretical basis for reducing potential inhibitory effects.
dc.language	English
dc.publisher	ELSEVIER SCIENCE SA
dc.relation.ispartof	Chemical Engineering Journal
dc.relation.isbasedon	10.1016/j.cej.2024.150167
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0904 Chemical Engineering, 0905 Civil Engineering, 0907 Environmental Engineering
dc.subject.classification	Chemical Engineering
dc.subject.classification	4004 Chemical engineering
dc.subject.classification	4011 Environmental engineering
dc.subject.classification	4016 Materials engineering
dc.title	Inhibition of norfloxacin on fermentative hydrogen production: Performance evaluation and metagenomic analysis
dc.type	Journal Article
utslib.citation.volume	486
utslib.for	0904 Chemical Engineering
utslib.for	0905 Civil Engineering
utslib.for	0907 Environmental Engineering
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Technology in Water and Wastewater (CTWW)
utslib.copyright.status	in_progress	*
dc.date.updated	2025-04-02T04:31:59Z
pubs.publication-status	Published
pubs.volume	486

Abstract:

Toxic chemicals can inhibit the dark fermentation process for renewable biohydrogen production. Norfloxacin (NOR), a typical antibiotic, is present in various feedstocks of dark fermentation, which has excellent antibacterial properties. It is hypothesized that NOR could cause adverse effects on fermentative hydrogen production, while current understanding of this subject is scare. This study investigated the potential inhibitory effect of NOR on dark hydrogen fermentation, and explored the underlying mechanisms from the perspectives of bacterial community structure and functional gene abundance. The results showed that NOR significantly reduced the hydrogen-producing capacity upon exposure to concentrations exceeding 10 mg/L. The hydrogen yield decreased from 1.56 to 0.27 H2/mol-glucose at NOR concentrations of 50 mg/L. NOR also prolonged the hydrogen-producing lag period. Bacterial community analysis revealed that NOR reduced the abundance of high-yielding H2-generating bacteria, such as Clostridium sp., while increasing the abundance of H2-consuming bacteria (e.g. Enterococcus sp.). Metagenomic analysis further indicated that crucial genes involved in glycolysis (e.g. HK, tal-pgi, pfk and PK) and hydrogen formation (e.g. por and E1.12.7.2) were remarkably downregulated under NOR exposure, essentially causing the inhibition of hydrogen production. This study contributes to a better understanding of how antibiotics inhibit dark hydrogen fermentation and provides a theoretical basis for reducing potential inhibitory effects.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/186464