Serotonin availability in rat colon is reduced during a Western diet model of obesity

Publisher:
American Physiological Society
Publication Type:
Journal article
Citation:
Liu, L. et al. 2012, 'Serotonin availability in rat colon is reduced during a Western diet model of obesity', American Journal of Physiology Gastrointestinal and Liver Physiology, vol. 303, no. 3, pp. G424-G434.
Issue Date:
2012
Full metadata record
Files in This Item:
Filename Description Size
Thumbnail2011007947OK.pdf1.15 MB
Adobe PDF
Constipation and slowed transit are associated with diet-induced obesity, although the mechanisms by which this occurs are unclear. Enterochromaffin (EC) cells within the intestinal epithelium respond to mechanical stimulation with the release of serotonin [5-hydroxytryptamine (5-HT)], which promotes transit. Thus our aim was to characterize 5-HT availability in the rat colon of a physiologically relevant model of diet-induced obesity. EC cell numbers were determined immunohistochemically in chow-fed (CF) and Western diet-fed (WD) rats, while electrochemical methods were used to measure mechanically evoked (peak) and steady-state (SS) 5-HT levels. Fluoxetine was used to block the 5-HT reuptake transporter (SERT), and the levels of mRNA for tryptophan hydroxylase 1 and SERT were determined by quantitative PCR, and SERT protein was determined by Western blot.
Please use this identifier to cite or link to this item: