ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastrointestinal LS174T epithelial cells

Crowe, A; Bebawy, M

ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastrointestinal LS174T epithelial cells

Crowe, A Bebawy, M

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Publication Type:: Journal Article
Citation:: European Journal of Pharmacology, 2012, 689 (1-3), pp. 204 - 210
Issue Date:: 2012-08-15

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Field	Value	Language
dc.contributor.author	Crowe, A	en_US
dc.contributor.author	Bebawy, M https://orcid.org/0000-0003-2606-921X	en_US
dc.date.available	2012-05-31	en_US
dc.date.issued	2012-08-15	en_US
dc.identifier.citation	European Journal of Pharmacology, 2012, 689 (1-3), pp. 204 - 210	en_US
dc.identifier.issn	0014-2999	en_US
dc.identifier.uri	http://hdl.handle.net/10453/23147
dc.description.abstract	The aim of this project was to show elevated P-glycoprotein (P-gp) expression decreasing bacterial association with LS174T human gastrointestinal cells, and that this effect could be reversed upon blocking functional P-gp efflux. Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Lactobacillus acidophilus and numerous strains of Escherichia coli, from commensal to enteropathogenic and enterohaemorrhagic strains (O157:H7) were fluorescently labelled and incubated on LS174T cultures either with or without P-gp amplification using rifampicin. PSC-833 was used as a potent functional P-gp blocking agent. Staphylococcus and Pseudomonas displayed the greatest association with the LS174T cells. Surprisingly, lactobacilli retained more fluorescence than enteropathogenic -E. coli in this system. Irrespective of attachment differences between the bacterial species, the increase in P-gp protein expression decreased bacterial fluorescence by 25-30%. This included the GFP-labelled E. coli, and enterohaemorrhagic E. coli (O157:H7). Blocking P-gp function through the co-administration of PSC-833 increased the amount of bacteria associated with P-gp expressing LS174T cells back to control levels. As most bacteria were affected to the same degree, irrespective of pathogenicity, it is unlikely that P-gp has a direct influence on adhesion of bacteria, and instead P-gp may be playing an indirect role by secreting a bank of endogenous factors or changing the local environment to one less suited to bacterial growth in general. Copyright © 2012 Published by Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	European Journal of Pharmacology	en_US
dc.relation.isbasedon	10.1016/j.ejphar.2012.05.047	en_US
dc.subject.classification	Behavioral Science & Comparative Psychology	en_US
dc.subject.classification	Pharmacology & Pharmacy	en_US
dc.subject.mesh	Gastrointestinal Tract	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Epithelial Cells	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Escherichia coli O157	en_US
dc.subject.mesh	Klebsiella pneumoniae	en_US
dc.subject.mesh	Staphylococcus aureus	en_US
dc.subject.mesh	Lactobacillus acidophilus	en_US
dc.subject.mesh	P-Glycoprotein	en_US
dc.subject.mesh	Attachment Sites, Microbiological	en_US
dc.subject.mesh	ATP Binding Cassette Transporter, Subfamily B	en_US
dc.subject.mesh	ATP Binding Cassette Transporter, Subfamily B, Member 1	en_US
dc.title	ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastrointestinal LS174T epithelial cells	en_US
dc.type	Journal Article
utslib.citation.volume	1-3	en_US
utslib.citation.volume	689	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
utslib.for	0801 Artificial Intelligence and Image Processing	en_US
utslib.for	1701 Psychology	en_US
utslib.for	1702 Cognitive Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	1-3	en_US
pubs.publication-status	Published	en_US
pubs.volume	689	en_US

Abstract:

The aim of this project was to show elevated P-glycoprotein (P-gp) expression decreasing bacterial association with LS174T human gastrointestinal cells, and that this effect could be reversed upon blocking functional P-gp efflux. Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Lactobacillus acidophilus and numerous strains of Escherichia coli, from commensal to enteropathogenic and enterohaemorrhagic strains (O157:H7) were fluorescently labelled and incubated on LS174T cultures either with or without P-gp amplification using rifampicin. PSC-833 was used as a potent functional P-gp blocking agent. Staphylococcus and Pseudomonas displayed the greatest association with the LS174T cells. Surprisingly, lactobacilli retained more fluorescence than enteropathogenic -E. coli in this system. Irrespective of attachment differences between the bacterial species, the increase in P-gp protein expression decreased bacterial fluorescence by 25-30%. This included the GFP-labelled E. coli, and enterohaemorrhagic E. coli (O157:H7). Blocking P-gp function through the co-administration of PSC-833 increased the amount of bacteria associated with P-gp expressing LS174T cells back to control levels. As most bacteria were affected to the same degree, irrespective of pathogenicity, it is unlikely that P-gp has a direct influence on adhesion of bacteria, and instead P-gp may be playing an indirect role by secreting a bank of endogenous factors or changing the local environment to one less suited to bacterial growth in general. Copyright © 2012 Published by Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/23147