Unravelling the complex venom landscapes of lethal Australian funnel-web spiders (Hexathelidae: Atracinae) using LC-MALDI-TOF mass spectrometry

King, G; Escoubas, P; Leblanc, M; Palagi, A; Wilson, D; Dutertre, S; Koh, J; Nicholson, G

Unravelling the complex venom landscapes of lethal Australian funnel-web spiders (Hexathelidae: Atracinae) using LC-MALDI-TOF mass spectrometry

King, G Escoubas, P Leblanc, M Palagi, A Wilson, D Dutertre, S Koh, J Nicholson, G

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: King, Glenn et al. 2013, 'Unravelling the complex venom landscapes of lethal Australian funnel-web spiders (Hexathelidae: Atracinae) using LC-MALDI-TOF mass spectrometry', Elsevier, vol. 80, pp. 292-310.
Issue Date:: 2013

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Field	Value	Language
dc.contributor.author	King, G	en_US
dc.contributor.author	Escoubas, P	en_US
dc.contributor.author	Leblanc, M	en_US
dc.contributor.author	Palagi, A	en_US
dc.contributor.author	Wilson, D	en_US
dc.contributor.author	Dutertre, S	en_US
dc.contributor.author	Koh, J	en_US
dc.contributor.author	Nicholson, G	en_US
dc.date.accessioned	2013-11-03T17:05:39Z
dc.date.available	2013-11-03T17:05:39Z
dc.date.issued	2013	en_US
dc.identifier	2012001532	en_US
dc.identifier.citation	King, Glenn et al. 2013, 'Unravelling the complex venom landscapes of lethal Australian funnel-web spiders (Hexathelidae: Atracinae) using LC-MALDI-TOF mass spectrometry', Elsevier, vol. 80, pp. 292-310.	en_US
dc.identifier.issn	1874-3919	en_US
dc.identifier.other	C1	en_US
dc.identifier.uri	http://hdl.handle.net/10453/24060
dc.description.abstract	Spider venoms represent vast sources of bioactive molecules whose diversity remains largely unknown. Indeed, only a small subset of species have been studied out of the ~43,000 extant spider species. The present study investigated inter- and intra-species venom complexity in 18 samples collected from a variety of lethal Australian funnel-web spiders (Mygalomorphae: Hexathelidae: Atracinae) using C4 reversed-phase separation coupled to offline MALDI-TOF mass spectrometry (LC-MALDI-TOF MS). An in-depth investigation focusing on four atracine venoms (male Illawarra wisharti, male and female Hadronyche cerberea, and female Hadronyche infensa Toowoomba) revealed, on average, ~800 peptides in female venoms while male venoms contained ~ 400 peptides, distributed across most HPLC fractions. This is significantly higher than previous estimates of peptide expression in mygalomorph venoms. These venoms also showed distinct intersexual as well as intra- and inter-species variation in peptide masses. Construction of both 3D and 2D contour plots revealed that peptide mass distributions in all 18 venoms were centered around the 3200a??5400 m/z range and to a lesser extent the 6600a??8200 m/z range, consistent with previously described hexatoxins. These findings highlight the extensive diversity of peptide toxins in Australian funnel-web spider venoms that that can be exploited as novel therapeutic and biopesticide lead molecules.	en_US
dc.publisher	Elsevier	en_US
dc.relation.ispartof	Journal of Proteomics	en_US
dc.relation.ispartof	Verified OK	en_US
dc.subject	Atrax species Hadronyche species Illawarra wisharti Spider venom LC-MALDI-TOF mass spectrometry Venom landscapes	en_US
dc.subject.classification	Toxicology (incl. Clinical Toxicology)	en_US
dc.title	Unravelling the complex venom landscapes of lethal Australian funnel-web spiders (Hexathelidae: Atracinae) using LC-MALDI-TOF mass spectrometry	en_US
dc.type	Journal article
utslib.citation.volume	80	en_US
utslib.location	Amsterdam	en_US
utslib.citation.startpage	292	en_US
utslib.citation.endpage	310	en_US
utslib.identifier.org	SCI.Faculty of Science	en_US
utslib.for	111506	en_US
utslib.percentage	50	en_US
utslib.copyright.status	closed_access

Abstract:

Spider venoms represent vast sources of bioactive molecules whose diversity remains largely unknown. Indeed, only a small subset of species have been studied out of the ~43,000 extant spider species. The present study investigated inter- and intra-species venom complexity in 18 samples collected from a variety of lethal Australian funnel-web spiders (Mygalomorphae: Hexathelidae: Atracinae) using C4 reversed-phase separation coupled to offline MALDI-TOF mass spectrometry (LC-MALDI-TOF MS). An in-depth investigation focusing on four atracine venoms (male Illawarra wisharti, male and female Hadronyche cerberea, and female Hadronyche infensa Toowoomba) revealed, on average, ~800 peptides in female venoms while male venoms contained ~ 400 peptides, distributed across most HPLC fractions. This is significantly higher than previous estimates of peptide expression in mygalomorph venoms. These venoms also showed distinct intersexual as well as intra- and inter-species variation in peptide masses. Construction of both 3D and 2D contour plots revealed that peptide mass distributions in all 18 venoms were centered around the 3200a??5400 m/z range and to a lesser extent the 6600a??8200 m/z range, consistent with previously described hexatoxins. These findings highlight the extensive diversity of peptide toxins in Australian funnel-web spider venoms that that can be exploited as novel therapeutic and biopesticide lead molecules.

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http://hdl.handle.net/10453/24060