Extragenic suppressor mutations that restore twitching motility to ﬁmL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels

Nolan, L; Beatson, S; Croft, L; Jones, P; George, A; Mattick, J; Turnbull, L; Whitchurch, C

Extragenic suppressor mutations that restore twitching motility to ﬁmL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels

Nolan, L Beatson, S Croft, L Jones, P George, A Mattick, J Turnbull, L Whitchurch, C

Permalink

Publisher:: Blackwell Publishing
Publication Type:: Article
Citation:: Nolan, L.M., Beatson, S.A., Croft, L., Jones, P.M., George, A.M., Mattick, J.S., Turnbull, L., and Whitchurch, C.B. (2012) Extragenic suppressor mutations that restore twitching motility to fimL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels. MicrobiologyOpen, 1(4): 490-501. 10.1002/mbo3.49
Issue Date:: 2012

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Extragenic suppressor mutations.pdfAdobe PDF (1.74 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Nolan, L
dc.contributor.author	Beatson, S
dc.contributor.author	Croft, L
dc.contributor.author	Jones, P
dc.contributor.author	George, A
dc.contributor.author	Mattick, J
dc.contributor.author	Turnbull, L
dc.contributor.author	Whitchurch, C
dc.date.accessioned	2014-03-28T05:50:53Z
dc.date.available	2014-03-28T05:50:53Z
dc.date.issued	2012
dc.identifier.citation	Nolan, L.M., Beatson, S.A., Croft, L., Jones, P.M., George, A.M., Mattick, J.S., Turnbull, L., and Whitchurch, C.B. (2012) Extragenic suppressor mutations that restore twitching motility to fimL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels. MicrobiologyOpen, 1(4): 490-501. 10.1002/mbo3.49	en_US
dc.identifier.issn	2045-8827
dc.identifier.uri	http://hdl.handle.net/10453/24212
dc.description.abstract	Cyclic AMP (cAMP) is a signaling molecule that is involved in the regulation of multiple virulence systems of the opportunistic pathogen Pseudomonas aeruginosa. The intracellular concentration of cAMP in P. aeruginosa cells is tightly controlled at the levels of cAMP synthesis and degradation through regulation of the activity and/or expression of the adenylate cyclases CyaA and CyaB or the cAMP phosphodiesterase CpdA. Interestingly, mutants of fimL, which usually demonstrate defective twitching motility, frequently revert to a wild-type twitching-motility phenotype presumably via the acquisition of an extragenic suppressor mutation(s). In this study, we have characterized five independent fimL twitching-motility revertants and have determined that all have increased intracellular cAMP levels compared with the parent fimL mutant. Whole-genome sequencing revealed that only one of these fimL revertants has acquired a loss-of-function mutation in cpdA that accounts for the elevated levels of intracellular cAMP. As mutation of cpdA did not account for the restoration of twitching motility observed in the other four fimL revertants, these observations suggest that there is at least another, as yet unidentified, site of extragenic suppressor mutation that can cause phenotypic reversion in fimL mutants and modulation of intracellular cAMP levels of P. aeruginosa.	en_US
dc.publisher	Blackwell Publishing	en_US
dc.relation.ispartof	MicrobiologyOpen
dc.relation.isbasedon	http://dx.doi.org/10.1002/mbo3.49
dc.subject	Microbiology
dc.title	Extragenic suppressor mutations that restore twitching motility to ﬁmL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels	en_US
dc.type	Article	en_US
utslib.citation.volume	1
utslib.citation.number	4
utslib.citation.startpage	490
utslib.citation.endpage	501
utslib.copyright.status	open_access

Abstract:

Cyclic AMP (cAMP) is a signaling molecule that is involved in the regulation of multiple virulence systems of the opportunistic pathogen Pseudomonas aeruginosa. The intracellular concentration of cAMP in P. aeruginosa cells is tightly controlled at the levels of cAMP synthesis and degradation through regulation of the activity and/or expression of the adenylate cyclases CyaA and CyaB or the cAMP phosphodiesterase CpdA. Interestingly, mutants of fimL, which usually demonstrate defective twitching motility, frequently revert to a wild-type twitching-motility phenotype presumably via the acquisition of an extragenic suppressor mutation(s). In this study, we have characterized five independent fimL twitching-motility revertants and have determined that all have increased intracellular cAMP levels compared with the parent fimL mutant. Whole-genome sequencing revealed that only one of these fimL revertants has acquired a loss-of-function mutation in cpdA that accounts for the elevated levels of intracellular cAMP. As mutation of cpdA did not account for the restoration of twitching motility observed in the other four fimL revertants, these observations suggest that there is at least another, as yet unidentified, site of extragenic suppressor mutation that can cause phenotypic reversion in fimL mutants and modulation of intracellular cAMP levels of P. aeruginosa.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/24212