Optimisation of derivatisation for the analysis of estrogenic compounds in water by solid-phase extraction gas chromatography-mass spectrometry

Zhang, ZL; Hibberd, A; Zhou, JL

Optimisation of derivatisation for the analysis of estrogenic compounds in water by solid-phase extraction gas chromatography-mass spectrometry

Zhang, ZL Hibberd, A Zhou, JL

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Publication Type:: Journal Article
Citation:: Analytica Chimica Acta, 2006, 577 (1), pp. 52 - 61
Issue Date:: 2006-09-01

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Field	Value	Language
dc.contributor.author	Zhang, ZL	en_US
dc.contributor.author	Hibberd, A	en_US
dc.contributor.author	Zhou, JL https://orcid.org/0000-0003-1393-1608	en_US
dc.date.available	2006-06-14	en_US
dc.date.issued	2006-09-01	en_US
dc.identifier.citation	Analytica Chimica Acta, 2006, 577 (1), pp. 52 - 61	en_US
dc.identifier.issn	0003-2670	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28158
dc.identifier.uri	http://hdl.handle.net/10453/33718
dc.description.abstract	An optimisation of derivatisation methods for the simultaneous determination of endocrine disrupting chemicals (EDCs) in water by solid-phase extraction (SPE) gas chromatography-mass spectrometry (GC-MS) was developed in this study. Seven highly potent EDCs including 17β-estradiol (E2), estrone (E1), 16α-hydroxyestrone, 17α-ethynylestradiol (EE2), bisphenol A, 4-nonylphenol and 4-tert-octylphenol were selected as the target compounds. The SPE technique, followed by the derivatisation with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was used for the extraction recoveries of compounds from water and effluent samples. The stability of the silylation derivatives under different reaction conditions was investigated. The combined use of BSTFA and pyridine as derivatisation reagents, together with the use of hexane as the final solvent, was preferred in order to generate more stable derivatives of EDCs. The relative response factor (RRF) of all derivatives except that of EE2 was stable 120 h after derivatisation. The addition of pyridine as derivatisation reagent with BSTFA can prevent the conversion of EE2 to other products during the reaction. Several parameters that may affect the recovery of EDCs, such as the SPE flow rate, and water properties including aquatic colloid content and surfactant concentration were tested. The results showed that the flow rate (1-25 mL min-1), colloid concentration (0-50 mg L-1) and surfactants concentration (0-10 μg L-1) did not cause significant decrease in the EDCs recovery. © 2006 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Analytica Chimica Acta	en_US
dc.relation.isbasedon	10.1016/j.aca.2006.06.029	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.title	Optimisation of derivatisation for the analysis of estrogenic compounds in water by solid-phase extraction gas chromatography-mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	577	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	0301 Analytical Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	577	en_US

Abstract:

An optimisation of derivatisation methods for the simultaneous determination of endocrine disrupting chemicals (EDCs) in water by solid-phase extraction (SPE) gas chromatography-mass spectrometry (GC-MS) was developed in this study. Seven highly potent EDCs including 17β-estradiol (E2), estrone (E1), 16α-hydroxyestrone, 17α-ethynylestradiol (EE2), bisphenol A, 4-nonylphenol and 4-tert-octylphenol were selected as the target compounds. The SPE technique, followed by the derivatisation with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was used for the extraction recoveries of compounds from water and effluent samples. The stability of the silylation derivatives under different reaction conditions was investigated. The combined use of BSTFA and pyridine as derivatisation reagents, together with the use of hexane as the final solvent, was preferred in order to generate more stable derivatives of EDCs. The relative response factor (RRF) of all derivatives except that of EE2 was stable 120 h after derivatisation. The addition of pyridine as derivatisation reagent with BSTFA can prevent the conversion of EE2 to other products during the reaction. Several parameters that may affect the recovery of EDCs, such as the SPE flow rate, and water properties including aquatic colloid content and surfactant concentration were tested. The results showed that the flow rate (1-25 mL min-1), colloid concentration (0-50 mg L-1) and surfactants concentration (0-10 μg L-1) did not cause significant decrease in the EDCs recovery. © 2006 Elsevier B.V. All rights reserved.

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