One pot synthesis of surface PEGylated core-shell microparticles by suspension polymerization with surface enrichment of Biotin/Avidin Conjugation

Ting, SRS; Nguyen, TLU; Stenzel, MH

One pot synthesis of surface PEGylated core-shell microparticles by suspension polymerization with surface enrichment of Biotin/Avidin Conjugation

Ting, SRS

Nguyen, TLU Stenzel, MH

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Publication Type:: Journal Article
Citation:: Macromolecular Bioscience, 2009, 9 (3), pp. 211 - 220
Issue Date:: 2009-03-10

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Field	Value	Language
dc.contributor.author	Ting, SRS https://orcid.org/0000-0002-7787-7906	en_US
dc.contributor.author	Nguyen, TLU	en_US
dc.contributor.author	Stenzel, MH	en_US
dc.date.issued	2009-03-10	en_US
dc.identifier.citation	Macromolecular Bioscience, 2009, 9 (3), pp. 211 - 220	en_US
dc.identifier.issn	1616-5187	en_US
dc.identifier.uri	http://hdl.handle.net/10453/31350
dc.description.abstract	Core-shell microparticles that consist of poly(vinyl neodecanoate) (VND) crosslinked with poly(ethylene glycol dimethacrylate) (EGDMA) as the core and poly(ethylene glycol methacrylate) (PEGMA) (M̄n = 360 or M̄n = 526 g · mol-1) as the shell have been synthesized using suspension polymerization by a conventional free radical polymerization process. Interfacial tension and stability tests show that PEGMA acts as an amphiphilic macromonomer and is located on the oil/water interface of the suspension system, thus forming an outer layer during the polymerization. Kinetic studies of the monomers' conversion of VND, EGDMA, and PEGMA have been carried out using 1H NMR spectroscopy. EGDMA and PEGMA were found to have faster reaction rates compared to VND. Moreover, scanning electron microscopy showed that the polymerization of these particles starts from the shell and finishes towards the core. Consequently, the resulting microsphere is found to have a multi-layer structure. Biotin was covalently bound to the surface by the PEGMA hydroxy groups. Conjugation of biotin with streptavidin PE (phycoerythrin) was subsequently carried out. Confocal microscopy was used to confirm the presence of fluorescing streptavidin. The amount of avidin conjugated to the microspheres was calculated by the release of a 2-(4-hydroxyphenylazo) benzoic acid /avidin complex using UV/vis spectroscopy. One avidin molecule was found to occupy 7 nm2 on the surface of the microspheres. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.	en_US
dc.relation.ispartof	Macromolecular Bioscience	en_US
dc.relation.isbasedon	10.1002/mabi.200800161	en_US
dc.subject.mesh	Polyethylene Glycols	en_US
dc.subject.mesh	Biotin	en_US
dc.subject.mesh	Phycoerythrin	en_US
dc.subject.mesh	Avidin	en_US
dc.subject.mesh	Streptavidin	en_US
dc.subject.mesh	Microspheres	en_US
dc.subject.mesh	Kinetics	en_US
dc.subject.mesh	Fluorescence	en_US
dc.title	One pot synthesis of surface PEGylated core-shell microparticles by suspension polymerization with surface enrichment of Biotin/Avidin Conjugation	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	9	en_US
utslib.for	0303 Macromolecular and Materials Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

Core-shell microparticles that consist of poly(vinyl neodecanoate) (VND) crosslinked with poly(ethylene glycol dimethacrylate) (EGDMA) as the core and poly(ethylene glycol methacrylate) (PEGMA) (M̄n = 360 or M̄n = 526 g · mol-1) as the shell have been synthesized using suspension polymerization by a conventional free radical polymerization process. Interfacial tension and stability tests show that PEGMA acts as an amphiphilic macromonomer and is located on the oil/water interface of the suspension system, thus forming an outer layer during the polymerization. Kinetic studies of the monomers' conversion of VND, EGDMA, and PEGMA have been carried out using 1H NMR spectroscopy. EGDMA and PEGMA were found to have faster reaction rates compared to VND. Moreover, scanning electron microscopy showed that the polymerization of these particles starts from the shell and finishes towards the core. Consequently, the resulting microsphere is found to have a multi-layer structure. Biotin was covalently bound to the surface by the PEGMA hydroxy groups. Conjugation of biotin with streptavidin PE (phycoerythrin) was subsequently carried out. Confocal microscopy was used to confirm the presence of fluorescing streptavidin. The amount of avidin conjugated to the microspheres was calculated by the release of a 2-(4-hydroxyphenylazo) benzoic acid /avidin complex using UV/vis spectroscopy. One avidin molecule was found to occupy 7 nm2 on the surface of the microspheres. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

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http://hdl.handle.net/10453/31350