Computational analysis, biochemical purification, and detection of tRNA-derived small RNA fragments
- Publication Type:
- Journal Article
- Methods in Molecular Biology, 2014, 1173 pp. 157 - 167
- Issue Date:
|Computational analysis, purification and detection of tRFs.pdf||Published Version||409.5 kB|
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
© Springer Science+Business Media New York 2014. The rapidly growing list of small RNA species generated by next-generation sequencing technologies has accelerated the development of new bioinformatics tools for their detection. Small RNAs generated from tRNAs, transfer RNA-derived fragments (tRFs), represent a novel challenge in accurately identifying and distinguishing them from random degradation products of tRNAs. Here, we describe a bioinformatics approach to detect tRFs in next-generation sequencing libraries. We also present a biochemical purification protocol for enriching 5′ tRFs and separating them from miRNAs. And finally, we suggest reliable methods for detecting and quantifying tRFs.
Please use this identifier to cite or link to this item: