An improved method for the simultaneous analysis of phenolic and steroidal estrogens in water and sediment

Hibberd, A; Maskaoui, K; Zhang, Z; Zhou, JL

An improved method for the simultaneous analysis of phenolic and steroidal estrogens in water and sediment

Hibberd, A Maskaoui, K Zhang, Z Zhou, JL

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Publication Type:: Journal Article
Citation:: Talanta, 2009, 77 (4), pp. 1315 - 1321
Issue Date:: 2009-02-15

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Field	Value	Language
dc.contributor.author	Hibberd, A	en_US
dc.contributor.author	Maskaoui, K	en_US
dc.contributor.author	Zhang, Z	en_US
dc.contributor.author	Zhou, JL https://orcid.org/0000-0003-1393-1608	en_US
dc.date.available	2008-09-04	en_US
dc.date.issued	2009-02-15	en_US
dc.identifier.citation	Talanta, 2009, 77 (4), pp. 1315 - 1321	en_US
dc.identifier.issn	0039-9140	en_US
dc.identifier.uri	http://hdl.handle.net/10453/33708
dc.identifier.uri	http://hdl.handle.net/10453/28155
dc.description.abstract	This paper describes an improved method for the extraction and analysis of seven endocrine disrupting chemicals with wide-ranging polarities from water and sediments using gas chromatography-tandem mass spectrometry (GC-MS/MS). The analytes were 4-tert-octylphenol, 4-nonylphenol, bisphenol A, estrone, 17β-estradiol, 17α-ethynylestradiol and 16α-hydroxyestrone. The optimised GC-MS/MS method produces increased selectivity and sensitivity compared to GC-MS, with limit of detection ranging from 0.01 to 0.49 ng L-1 in water and from 0.05 to 0.14 ng g-1 in sediment. Extraction from aqueous samples was performed by solid-phase extraction (SPE) and from sediment samples by microwave-assisted extraction (MAE). The improved method for the clean-up of sediment extracts carried out by SPE enhanced EDC recovery (86-102%) while reducing matrix interference and sample drying time. Derivatisation of final sample extracts was achieved using N,O-bis(trimethylsilyl)trifluoroacetamide and pyridine, and their stability was enhanced by reconstituting the derivatised extracts with hexane. The method was validated by spiking experiments which showed good recovery and reproducibility. The method was applied to samples taken from the Medway estuary in Kent, UK, where non-conservative behaviour of EDCs was demonstrated. © 2008 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Talanta	en_US
dc.relation.isbasedon	10.1016/j.talanta.2008.09.006	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Water	en_US
dc.subject.mesh	Phenols	en_US
dc.subject.mesh	Pyridines	en_US
dc.subject.mesh	Steroids	en_US
dc.subject.mesh	Estrogens	en_US
dc.subject.mesh	Solvents	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Chromatography, Gas	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Geologic Sediments	en_US
dc.subject.mesh	Microwaves	en_US
dc.subject.mesh	Endocrine Disruptors	en_US
dc.subject.mesh	Mass Spectrometry	en_US
dc.subject.mesh	Solid Phase Extraction	en_US
dc.title	An improved method for the simultaneous analysis of phenolic and steroidal estrogens in water and sediment	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	77	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	0301 Analytical Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	77	en_US

Abstract:

This paper describes an improved method for the extraction and analysis of seven endocrine disrupting chemicals with wide-ranging polarities from water and sediments using gas chromatography-tandem mass spectrometry (GC-MS/MS). The analytes were 4-tert-octylphenol, 4-nonylphenol, bisphenol A, estrone, 17β-estradiol, 17α-ethynylestradiol and 16α-hydroxyestrone. The optimised GC-MS/MS method produces increased selectivity and sensitivity compared to GC-MS, with limit of detection ranging from 0.01 to 0.49 ng L-1 in water and from 0.05 to 0.14 ng g-1 in sediment. Extraction from aqueous samples was performed by solid-phase extraction (SPE) and from sediment samples by microwave-assisted extraction (MAE). The improved method for the clean-up of sediment extracts carried out by SPE enhanced EDC recovery (86-102%) while reducing matrix interference and sample drying time. Derivatisation of final sample extracts was achieved using N,O-bis(trimethylsilyl)trifluoroacetamide and pyridine, and their stability was enhanced by reconstituting the derivatised extracts with hexane. The method was validated by spiking experiments which showed good recovery and reproducibility. The method was applied to samples taken from the Medway estuary in Kent, UK, where non-conservative behaviour of EDCs was demonstrated. © 2008 Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/28155