Laboratory culture and life-cycle experiments with the benthic amphipod Melita plumulosa (Zeidler).

Hyne, RV; Gale, SA; King, CK

Laboratory culture and life-cycle experiments with the benthic amphipod Melita plumulosa (Zeidler).

Hyne, RV Gale, SA King, CK

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Publication Type:: Journal Article
Citation:: Environ Toxicol Chem, 2005, 24 (8), pp. 2065 - 2073
Issue Date:: 2005-08

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Field	Value	Language
dc.contributor.author	Hyne, RV	en_US
dc.contributor.author	Gale, SA	en_US
dc.contributor.author	King, CK	en_US
dc.date.issued	2005-08	en_US
dc.identifier.citation	Environ Toxicol Chem, 2005, 24 (8), pp. 2065 - 2073	en_US
dc.identifier.issn	0730-7268	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3624
dc.description.abstract	In Australia, the collection of estuarine invertebrates from the field for whole-sediment toxicity tests is hindered because of temporal variability in their population densities and distributions. The present study aimed to develop culturing procedures for Melita plumulosa (Zeidler), an epibenthic and intertidal, deposit-feeding amphipod that is native to the southeastern coast of Australia. During a 28-d chronic exposure, the species was tested under a range of salinities (5-35 per thousand), temperatures (14-25 degrees C), and sediment particle sizes (sand to silt). Optimal culture conditions with respect to salinity, temperature, sediment particle size, feeding, and light regimes were determined. Compared to survival, amphipod growth and fertility were better predictors of optimal culture conditions. A life-history experiment was undertaken at the initial culture conditions of 22 degrees C and 35 per thousand salinity to establish the age at maturity, length of reproductive cycle, and life span for each sex. Under these test conditions, posthatch female M. plumulosa released their first offspring at seven weeks, after which each female produced an average of nine juveniles every 16 d. Male M. plumulosa had an average life span of eight months, whereas females had an average life span of 11 months. Optimal culturing conditions established in the present study have been incorporated into toxicity test procedures with this species and are being used to maximize reproductive output of this species in laboratory cultures to provide a supply of juveniles for routine use in whole-sediment toxicity tests.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Environ Toxicol Chem	en_US
dc.relation.isbasedon	10.1897/04-409r1.1	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Amphipoda	en_US
dc.subject.mesh	Sodium Chloride	en_US
dc.subject.mesh	Temperature	en_US
dc.subject.mesh	Life Cycle Stages	en_US
dc.subject.mesh	Particle Size	en_US
dc.title	Laboratory culture and life-cycle experiments with the benthic amphipod Melita plumulosa (Zeidler).	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	24	en_US
utslib.location.activity	United States	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	24	en_US

Abstract:

In Australia, the collection of estuarine invertebrates from the field for whole-sediment toxicity tests is hindered because of temporal variability in their population densities and distributions. The present study aimed to develop culturing procedures for Melita plumulosa (Zeidler), an epibenthic and intertidal, deposit-feeding amphipod that is native to the southeastern coast of Australia. During a 28-d chronic exposure, the species was tested under a range of salinities (5-35 per thousand), temperatures (14-25 degrees C), and sediment particle sizes (sand to silt). Optimal culture conditions with respect to salinity, temperature, sediment particle size, feeding, and light regimes were determined. Compared to survival, amphipod growth and fertility were better predictors of optimal culture conditions. A life-history experiment was undertaken at the initial culture conditions of 22 degrees C and 35 per thousand salinity to establish the age at maturity, length of reproductive cycle, and life span for each sex. Under these test conditions, posthatch female M. plumulosa released their first offspring at seven weeks, after which each female produced an average of nine juveniles every 16 d. Male M. plumulosa had an average life span of eight months, whereas females had an average life span of 11 months. Optimal culturing conditions established in the present study have been incorporated into toxicity test procedures with this species and are being used to maximize reproductive output of this species in laboratory cultures to provide a supply of juveniles for routine use in whole-sediment toxicity tests.

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http://hdl.handle.net/10453/3624