Targeted delivery of siRNA using glycopolymer

Ting, SS; Min, EH; Nguyen, HT; Stenzel, MH; Hutvagner, G

Targeted delivery of siRNA using glycopolymer

Ting, SS

Min, EH Nguyen, HT

Stenzel, MH Hutvagner, G

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Publication Type:: Conference Proceeding
Citation:: 2015
Issue Date:: 2015-07-12

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Field	Value	Language
dc.contributor.author	Ting, SS https://orcid.org/0000-0002-7787-7906	en_US
dc.contributor.author	Min, EH	en_US
dc.contributor.author	Nguyen, HT https://orcid.org/0000-0003-3373-8178	en_US
dc.contributor.author	Stenzel, MH	en_US
dc.contributor.author	Hutvagner, G https://orcid.org/0000-0002-7231-9446	en_US
dc.date	2015-07-12	en_US
dc.date.available	2015-04-08	en_US
dc.date.issued	2015-07-12	en_US
dc.identifier.citation	2015	en_US
dc.identifier.uri	http://hdl.handle.net/10453/36543
dc.description.abstract	Liver is an essential part of the human biological system as it serves to detoxify, synthesize protein and produce biochemicals necessary for digestion. However, there have been common liver diseases namely, hepatitis (A, B, C, and E), fatty liver, cirrhosis and ultimately liver cancer. RNA interference (RNAi) mediated through double-stranded small interfering RNA (siRNA) pave the way to knockdown disease causing gene.1 Nevertheless, effective delivery of siRNA is an arduous task as they are very prone to degradation and are difficult to target specific cells. Glycopolymers are carbohydrates based polymers that recognise biological receptors on cells.2 This project focuses on the design of synthetic glycopolymer architectures using reversible addition-fragmentation transfer (RAFT) polymerization of sugar containing monomers for conjugations of siRNA. Galactose based monomer are selected here, as liver cancer cells over-expressed asialoglycoproteins, which are galactose recognising receptors. Moreover, synthetic delivery system has been reported to protect enzymatic degradation of therapeutics during delivery in the biological enviroment.3 Figure 1 shows the synthetic approach towards glycopolymers for the conjugation of siRNA by using a 4-Cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl] pentanoic acid (CPDT) RAFT agent, 4,4′-Azobis(4-cyanovaleric acid) (ACVA) initiator polymerized in dioxane. Figure 2 display the increased in molecular weights of polymers with increasing monomer conversions.	en_US
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1054011
dc.relation.ispartof	35th Australasian Polymer Symposium	en_US
dc.title	Targeted delivery of siRNA using glycopolymer	en_US
dc.type	Conference Proceeding
utslib.location	Australia	en_US
utslib.location.activity	Gold Coast, Australia	en_US
utslib.for	0903 Biomedical Engineering	en_US
dc.location.activity	Gold Coast, Australia
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.consider-herdc	false	en_US
pubs.finish-date	2015-07-15	en_US
pubs.place-of-publication	Australia	en_US
pubs.publication-status	Published	en_US
pubs.start-date	2015-07-12	en_US

Abstract:

Liver is an essential part of the human biological system as it serves to detoxify, synthesize protein and produce biochemicals necessary for digestion. However, there have been common liver diseases namely, hepatitis (A, B, C, and E), fatty liver, cirrhosis and ultimately liver cancer. RNA interference (RNAi) mediated through double-stranded small interfering RNA (siRNA) pave the way to knockdown disease causing gene.1 Nevertheless, effective delivery of siRNA is an arduous task as they are very prone to degradation and are difficult to target specific cells. Glycopolymers are carbohydrates based polymers that recognise biological receptors on cells.2 This project focuses on the design of synthetic glycopolymer architectures using reversible addition-fragmentation transfer (RAFT) polymerization of sugar containing monomers for conjugations of siRNA. Galactose based monomer are selected here, as liver cancer cells over-expressed asialoglycoproteins, which are galactose recognising receptors. Moreover, synthetic delivery system has been reported to protect enzymatic degradation of therapeutics during delivery in the biological enviroment.3 Figure 1 shows the synthetic approach towards glycopolymers for the conjugation of siRNA by using a 4-Cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl] pentanoic acid (CPDT) RAFT agent, 4,4′-Azobis(4-cyanovaleric acid) (ACVA) initiator polymerized in dioxane. Figure 2 display the increased in molecular weights of polymers with increasing monomer conversions.

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http://hdl.handle.net/10453/36543