Indicators of biofilm development and activity in constructed wetlands microcosms

Ragusa, SR; McNevin, D; Qasem, S; Mitchell, C

Indicators of biofilm development and activity in constructed wetlands microcosms

Ragusa, SR McNevin, D

Qasem, S Mitchell, C

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Publication Type:: Journal Article
Citation:: Water Research, 2004, 38 (12), pp. 2865 - 2873
Issue Date:: 2004-01-01

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Field	Value	Language
dc.contributor.author	Ragusa, SR	en_US
dc.contributor.author	McNevin, D https://orcid.org/0000-0003-1665-3367	en_US
dc.contributor.author	Qasem, S	en_US
dc.contributor.author	Mitchell, C https://orcid.org/0000-0001-9842-540X	en_US
dc.date.available	2004-03-26	en_US
dc.date.issued	2004-01-01	en_US
dc.identifier.citation	Water Research, 2004, 38 (12), pp. 2865 - 2873	en_US
dc.identifier.issn	0043-1354	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3938
dc.description.abstract	Methods to measure protein, exopolysaccharide, viable cell number and INT reduction activity were tested on biofilm growing in a wastewater batch reactor. They were shown to be meaningful indicators of biofilm growth and correlated well with each other. Protein, exopolysaccharide, viable cells and INT reduction rates increased linearly over time. Viable cell number exhibited strong linear correlations with protein (R2=0.98) and exopolysaccharide (R 2=0.99) while INT reduction rate was somewhat less well correlated (R2=0.90). Our results indicate production rates of 0.91×10-7μg EPS per viable cell and 1.0×10 -7μg protein per viable cell. Protein and polysaccharide specific INT reduction rates decreased by approximately 50%, whereas viable cell specific INT reduction rates decreased by 65% and the protein to polysaccharide ratio stayed relatively constant at between 1.1 and 1.2 as the biofilm developed. Measurement of protein, polysaccharide, viable cells and INT reduction rate at depth within the bioreactor showed that they were concentrated in the top 1cm of the influent end of the reactor and each decreased to a base level within 4.5cm of the inlet. Protein to polysaccharide ratios increased with depth in the reactor and the specific INT reduction rates were maximal at 4.5cm depth. The results indicate that the biomass can take upwards of 100 days to stabilize during batch (fill and draw) operation of subsurface wetlands and that the relative ratios of biomass components remain relatively constant during biofilm growth. Also, it appears that filtration of suspended solids results in biomass concentration at the inlet to the wetland. © 2004 Elsevier Ltd. All rights reserved.	en_US
dc.relation	http://purl.org/au-research/grants/arc/A00104349
dc.relation.ispartof	Water Research	en_US
dc.relation.isbasedon	10.1016/j.watres.2004.03.039	en_US
dc.subject.classification	Environmental Engineering	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Polysaccharides	en_US
dc.subject.mesh	Proteins	en_US
dc.subject.mesh	Bioreactors	en_US
dc.subject.mesh	Ecosystem	en_US
dc.subject.mesh	Biomass	en_US
dc.subject.mesh	Waste Disposal, Fluid	en_US
dc.subject.mesh	Water Purification	en_US
dc.subject.mesh	Kinetics	en_US
dc.title	Indicators of biofilm development and activity in constructed wetlands microcosms	en_US
dc.type	Journal Article
utslib.citation.volume	12	en_US
utslib.citation.volume	38	en_US
utslib.for	0907 Environmental Engineering	en_US
utslib.for	0799 Other Agricultural and Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/DVC (Research)
pubs.organisational-group	/University of Technology Sydney/DVC (Research)/Institute For Sustainable Futures
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Strength - ISF - Institute for Sustainable Futures
utslib.copyright.status	closed_access
pubs.issue	12	en_US
pubs.publication-status	Published	en_US
pubs.volume	38	en_US

Abstract:

Methods to measure protein, exopolysaccharide, viable cell number and INT reduction activity were tested on biofilm growing in a wastewater batch reactor. They were shown to be meaningful indicators of biofilm growth and correlated well with each other. Protein, exopolysaccharide, viable cells and INT reduction rates increased linearly over time. Viable cell number exhibited strong linear correlations with protein (R2=0.98) and exopolysaccharide (R 2=0.99) while INT reduction rate was somewhat less well correlated (R2=0.90). Our results indicate production rates of 0.91×10-7μg EPS per viable cell and 1.0×10 -7μg protein per viable cell. Protein and polysaccharide specific INT reduction rates decreased by approximately 50%, whereas viable cell specific INT reduction rates decreased by 65% and the protein to polysaccharide ratio stayed relatively constant at between 1.1 and 1.2 as the biofilm developed. Measurement of protein, polysaccharide, viable cells and INT reduction rate at depth within the bioreactor showed that they were concentrated in the top 1cm of the influent end of the reactor and each decreased to a base level within 4.5cm of the inlet. Protein to polysaccharide ratios increased with depth in the reactor and the specific INT reduction rates were maximal at 4.5cm depth. The results indicate that the biomass can take upwards of 100 days to stabilize during batch (fill and draw) operation of subsurface wetlands and that the relative ratios of biomass components remain relatively constant during biofilm growth. Also, it appears that filtration of suspended solids results in biomass concentration at the inlet to the wetland. © 2004 Elsevier Ltd. All rights reserved.

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http://hdl.handle.net/10453/3938