Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate

Protti, DA; Di Marco, S; Huang, JY; Vonhoff, CR; Nguyen, V; Solomon, SG

Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate

Protti, DA Di Marco, S Huang, JY Vonhoff, CR Nguyen, V

Solomon, SG

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Publication Type:: Journal Article
Citation:: Journal of Physiology, 2014, 592 (1), pp. 49 - 65
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Protti, DA	en_US
dc.contributor.author	Di Marco, S	en_US
dc.contributor.author	Huang, JY	en_US
dc.contributor.author	Vonhoff, CR	en_US
dc.contributor.author	Nguyen, V https://orcid.org/0000-0003-3744-1523	en_US
dc.contributor.author	Solomon, SG	en_US
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	Journal of Physiology, 2014, 592 (1), pp. 49 - 65	en_US
dc.identifier.issn	0022-3751	en_US
dc.identifier.uri	http://hdl.handle.net/10453/47907
dc.description.abstract	The centre-surround organisation of receptive fields is a feature of most retinal ganglion cells (RGCs) and is critical for spatial discrimination and contrast detection. Although lateral inhibitory processes are known to be important in generating the receptive field surround, the contribution of each of the two synaptic layers in the primate retina remains unclear. Here we studied the spatial organisation of excitatory and inhibitory synaptic inputs onto ON and OFF ganglion cells in the primate retina. All RGCs showed an increase in excitation in response to stimulus of preferred polarity. Inhibition onto RGCs comprised two types of responses to preferred polarity: some RGCs showed an increase in inhibition whilst others showed removal of tonic inhibition. Excitatory inputs were strongly spatially tuned but inhibitory inputs showed more variable organisation: in some neurons they were as strongly tuned as excitation, and in others inhibitory inputs showed no spatial tuning. We targeted one source of inner retinal inhibition by functionally ablating spiking amacrine cells with bath application of tetrodotoxin (TTX). TTX significantly reduced the spatial tuning of excitatory inputs. In addition, TTX reduced inhibition onto those RGCs where a stimulus of preferred polarity increased inhibition. Reconstruction of the spatial tuning properties by somatic injection of excitatory and inhibitory synaptic conductances verified that TTX-mediated inhibition onto bipolar cells increases the strength of the surround in RGC spiking output. These results indicate that in the primate retina inhibitory mechanisms in the inner plexiform layer sharpen the spatial tuning of ganglion cells. © 2013 The Physiological Society.	en_US
dc.relation.ispartof	Journal of Physiology	en_US
dc.relation.isbasedon	10.1113/jphysiol.2013.257352	en_US
dc.subject.classification	Physiology	en_US
dc.subject.mesh	Amacrine Cells	en_US
dc.subject.mesh	Retinal Ganglion Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Callithrix	en_US
dc.subject.mesh	Tetrodotoxin	en_US
dc.subject.mesh	Sodium Channel Blockers	en_US
dc.subject.mesh	Excitatory Postsynaptic Potentials	en_US
dc.subject.mesh	Visual Fields	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Inhibitory Postsynaptic Potentials	en_US
dc.title	Inner retinal inhibition shapes the receptive field of retinal ganglion cells in primate	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	592	en_US
utslib.for	1113 Opthalmology and Optometry	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	592	en_US

Abstract:

The centre-surround organisation of receptive fields is a feature of most retinal ganglion cells (RGCs) and is critical for spatial discrimination and contrast detection. Although lateral inhibitory processes are known to be important in generating the receptive field surround, the contribution of each of the two synaptic layers in the primate retina remains unclear. Here we studied the spatial organisation of excitatory and inhibitory synaptic inputs onto ON and OFF ganglion cells in the primate retina. All RGCs showed an increase in excitation in response to stimulus of preferred polarity. Inhibition onto RGCs comprised two types of responses to preferred polarity: some RGCs showed an increase in inhibition whilst others showed removal of tonic inhibition. Excitatory inputs were strongly spatially tuned but inhibitory inputs showed more variable organisation: in some neurons they were as strongly tuned as excitation, and in others inhibitory inputs showed no spatial tuning. We targeted one source of inner retinal inhibition by functionally ablating spiking amacrine cells with bath application of tetrodotoxin (TTX). TTX significantly reduced the spatial tuning of excitatory inputs. In addition, TTX reduced inhibition onto those RGCs where a stimulus of preferred polarity increased inhibition. Reconstruction of the spatial tuning properties by somatic injection of excitatory and inhibitory synaptic conductances verified that TTX-mediated inhibition onto bipolar cells increases the strength of the surround in RGC spiking output. These results indicate that in the primate retina inhibitory mechanisms in the inner plexiform layer sharpen the spatial tuning of ganglion cells. © 2013 The Physiological Society.

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http://hdl.handle.net/10453/47907