Simultaneous analysis of multiple classes of antimicrobials in environmental water samples using SPE coupled with UHPLC-ESI-MS/MS and isotope dilution

Tran, NH; Chen, H; Do, TV; Reinhard, M; Ngo, HH; He, Y; Gin, KYH

Simultaneous analysis of multiple classes of antimicrobials in environmental water samples using SPE coupled with UHPLC-ESI-MS/MS and isotope dilution

Tran, NH Chen, H Do, TV Reinhard, M Ngo, HH

He, Y Gin, KYH

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Publication Type:: Journal Article
Citation:: Talanta, 2016, 159 pp. 163 - 173
Issue Date:: 2016-10-01

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Field	Value	Language
dc.contributor.author	Tran, NH	en_US
dc.contributor.author	Chen, H	en_US
dc.contributor.author	Do, TV	en_US
dc.contributor.author	Reinhard, M	en_US
dc.contributor.author	Ngo, HH https://orcid.org/0000-0002-0296-2866	en_US
dc.contributor.author	He, Y	en_US
dc.contributor.author	Gin, KYH	en_US
dc.date.available	2020-05-25T19:14:01Z
dc.date.issued	2016-10-01	en_US
dc.identifier.citation	Talanta, 2016, 159 pp. 163 - 173	en_US
dc.identifier.issn	0039-9140	en_US
dc.identifier.uri	http://hdl.handle.net/10453/61392
dc.description.abstract	© 2016 Elsevier B.V. A robust and sensitive analytical method was developed for the simultaneous analysis of 21 target antimicrobials in different environmental water samples. Both single SPE and tandem SPE cartridge systems were investigated to simultaneously extract multiple classes of antimicrobials. Experimental results showed that good extraction efficiencies (84.5-105.6%) were observed for the vast majority of the target analytes when extraction was performed using the tandem SPE cartridge (SB+HR-X) system under an extraction pH of 3.0. HPLC-MS/MS parameters were optimized for simultaneous analysis of all the target analytes in a single injection. Quantification of target antimicrobials in water samples was accomplished using 15 isotopically labeled internal standards (ILISs), which allowed the efficient compensation of the losses of target analytes during sample preparation and correction of matrix effects during UHPLC-MS/MS as well as instrument fluctuations in MS/MS signal intensity. Method quantification limit (MQL) for most target analytes based on SPE was below 5 ng/L for surface waters, 10 ng/L for treated wastewater effluents, and 15 ng/L for raw wastewater. The method was successfully applied to detect and quantify the occurrence of the target analytes in raw influent, treated effluent and surface water samples.	en_US
dc.relation.ispartof	Talanta	en_US
dc.relation.isbasedon	10.1016/j.talanta.2016.06.006	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Water	en_US
dc.subject.mesh	Isotopes	en_US
dc.subject.mesh	Anti-Infective Agents	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Chromatography, High Pressure Liquid	en_US
dc.subject.mesh	Environmental Monitoring	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.subject.mesh	Solid Phase Extraction	en_US
dc.title	Simultaneous analysis of multiple classes of antimicrobials in environmental water samples using SPE coupled with UHPLC-ESI-MS/MS and isotope dilution	en_US
dc.type	Journal Article
utslib.citation.volume	159	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	159	en_US

Abstract:

© 2016 Elsevier B.V. A robust and sensitive analytical method was developed for the simultaneous analysis of 21 target antimicrobials in different environmental water samples. Both single SPE and tandem SPE cartridge systems were investigated to simultaneously extract multiple classes of antimicrobials. Experimental results showed that good extraction efficiencies (84.5-105.6%) were observed for the vast majority of the target analytes when extraction was performed using the tandem SPE cartridge (SB+HR-X) system under an extraction pH of 3.0. HPLC-MS/MS parameters were optimized for simultaneous analysis of all the target analytes in a single injection. Quantification of target antimicrobials in water samples was accomplished using 15 isotopically labeled internal standards (ILISs), which allowed the efficient compensation of the losses of target analytes during sample preparation and correction of matrix effects during UHPLC-MS/MS as well as instrument fluctuations in MS/MS signal intensity. Method quantification limit (MQL) for most target analytes based on SPE was below 5 ng/L for surface waters, 10 ng/L for treated wastewater effluents, and 15 ng/L for raw wastewater. The method was successfully applied to detect and quantify the occurrence of the target analytes in raw influent, treated effluent and surface water samples.

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http://hdl.handle.net/10453/61392