Variation In Antioxidant Gene Expression In The Scleractinian Coral Acropora Millepora Under Laboratory Thermal Stress

Csaszar, N; Seneca, F; Van Oppen, M

Variation In Antioxidant Gene Expression In The Scleractinian Coral Acropora Millepora Under Laboratory Thermal Stress

Csaszar, N Seneca, F Van Oppen, M

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Publisher:: Inter-Research
Publication Type:: Journal Article
Citation:: Csaszar Nikolaus, Seneca Fo, and Van Oppen Madeleine 2009, 'Variation In Antioxidant Gene Expression In The Scleractinian Coral Acropora Millepora Under Laboratory Thermal Stress', Inter-Research, vol. 392, no. NA, pp. 93-102.
Issue Date:: 2009

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Field	Value	Language
dc.contributor.author	Csaszar, N	en_US
dc.contributor.author	Seneca, F	en_US
dc.contributor.author	Van Oppen, M	en_US
dc.date.accessioned	2010-05-28T09:45:10Z
dc.date.available	2010-05-28T09:45:10Z
dc.date.issued	2009	en_US
dc.identifier	2009002402	en_US
dc.identifier.citation	Csaszar Nikolaus, Seneca Fo, and Van Oppen Madeleine 2009, 'Variation In Antioxidant Gene Expression In The Scleractinian Coral Acropora Millepora Under Laboratory Thermal Stress', Inter-Research, vol. 392, no. NA, pp. 93-102.	en_US
dc.identifier.issn	0171-8630	en_US
dc.identifier.other	C1	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8739
dc.description.abstract	The effects of climate change over the last few decades have pushed the majority of reef-building corals close to their upper thermal limit. Upon exposure to thermal stress, the intra-cellular formation of harmful oxygen intermediates can lead to the disruption of the obligate symbiosis between the coral host and their dinoflagellate endosymbionts (zooxanthellae), a process known as coral bleaching. Applications of molecular techniques to cnidarian research have recently enhanced our understanding of the magnitude of corals? transcriptional response to various stressors. In the present study, we developed a quantitative real-time polymerase chain reaction assay to assess expression levels of 4 genes involved in the corals? oxidative stress response (HSP70, MnSOD, ferritin, Zn2+-metalloprotease) after exposure to laboratory-controlled thermal stress. Using the Indo-Pacific reef coral Acropora millepora, our study provides the first population-scale analysis of antioxidant gene expression in coral. Despite the significant up-regulation of those 4 genes in the thermally stressed samples relative to non-stressed samples, our results show that there is an enormous intra- as well as inter-colony variation in transcript abundance at a particular point in time. We discuss the potential roles of ferritin and Zn2+-metalloprotease in the break down of the intra-cellular Fe2+-homeostasis and in coral host cell detachment, respectively, during bleaching conditions. Our results emphasise the importance of measuring inter-individual variation to gain an insight into the population response to a common and increasingly encountered environmental stressor.	en_US
dc.publisher	Inter-Research	en_US
dc.relation.ispartof	Marine Ecology Progress Series	en_US
dc.relation.ispartof	Verified OK	en_US
dc.relation.isbasedon	http://dx.doi.org/10.3354/meps08194	en_US
dc.subject	Oxidative stress ? Gene expression ? Variation ? Coral bleaching ? Acropora millepora ? Real-time PCR ? Magnetic beads	en_US
dc.subject.classification	Marine and Estuarine Ecology (incl. Marine Ichthyology)	en_US
dc.title	Variation In Antioxidant Gene Expression In The Scleractinian Coral Acropora Millepora Under Laboratory Thermal Stress	en_US
dc.type	Journal article
utslib.citation.volume	392	en_US
utslib.location	Oldendorf Luhe	en_US
utslib.citation.startpage	93	en_US
utslib.citation.endpage	102	en_US
utslib.identifier.org	SCI.Faculty of Science	en_US
utslib.for	060205	en_US
utslib.percentage	100	en_US
dc.location.activity	ISI:000271828700008	en_US
utslib.copyright.status	closed_access
pubs.declined	1970-01-01T00:00:00.0+1000

Abstract:

The effects of climate change over the last few decades have pushed the majority of reef-building corals close to their upper thermal limit. Upon exposure to thermal stress, the intra-cellular formation of harmful oxygen intermediates can lead to the disruption of the obligate symbiosis between the coral host and their dinoflagellate endosymbionts (zooxanthellae), a process known as coral bleaching. Applications of molecular techniques to cnidarian research have recently enhanced our understanding of the magnitude of corals? transcriptional response to various stressors. In the present study, we developed a quantitative real-time polymerase chain reaction assay to assess expression levels of 4 genes involved in the corals? oxidative stress response (HSP70, MnSOD, ferritin, Zn2+-metalloprotease) after exposure to laboratory-controlled thermal stress. Using the Indo-Pacific reef coral Acropora millepora, our study provides the first population-scale analysis of antioxidant gene expression in coral. Despite the significant up-regulation of those 4 genes in the thermally stressed samples relative to non-stressed samples, our results show that there is an enormous intra- as well as inter-colony variation in transcript abundance at a particular point in time. We discuss the potential roles of ferritin and Zn2+-metalloprotease in the break down of the intra-cellular Fe2+-homeostasis and in coral host cell detachment, respectively, during bleaching conditions. Our results emphasise the importance of measuring inter-individual variation to gain an insight into the population response to a common and increasingly encountered environmental stressor.

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http://hdl.handle.net/10453/8739