Using the inflammatory cell infiltrate to estimate the age of human burn wounds A review and immunohistochemical study

Tarran, S; Langlois, N; Dziewulski, P; Sztynda, T

Using the inflammatory cell infiltrate to estimate the age of human burn wounds A review and immunohistochemical study

Tarran, S Langlois, N Dziewulski, P Sztynda, T

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Publisher:: Chiltern Publishing
Publication Type:: Journal Article
Citation:: Tarran Sarah et al. 2006, 'Using the inflammatory cell infiltrate to estimate the age of human burn wounds A review and immunohistochemical study', Chiltern Publishing, vol. 46, no. 2, pp. 115-126.
Issue Date:: 2006

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Field	Value	Language
dc.contributor.author	Tarran, S	en_US
dc.contributor.author	Langlois, N	en_US
dc.contributor.author	Dziewulski, P	en_US
dc.contributor.author	Sztynda, T	en_US
dc.date.accessioned	2009-08-20T13:01:32Z
dc.date.available	2009-08-20T13:01:32Z
dc.date.issued	2006	en_US
dc.identifier	2006004001	en_US
dc.identifier.citation	Tarran Sarah et al. 2006, 'Using the inflammatory cell infiltrate to estimate the age of human burn wounds A review and immunohistochemical study', Chiltern Publishing, vol. 46, no. 2, pp. 115-126.	en_US
dc.identifier.issn	0025-8024	en_US
dc.identifier.other	C1	en_US
dc.identifier.uri	http://hdl.handle.net/10453/945
dc.description.abstract	The burn wound healing process, which is reviewed in this paper, has features that differ from the healing of incised cutaneous wounds. This study used immunohistochemical staining and cell counting to examine the inflammatory cell response in biopsy samples of burn wounds from live human subjects obtained at six hours until 23 days after injury in order to determine how the age of a burn could be estimated. Acute inflammatory cells predominated in samples taken six hours to two days after injury. However, neutrophils were often minimal in early samples or could be present late. Elevated numbers of macrophages tended to be encountered from days 2 to 20, but it was not uncommon to observe a minimal or absent macrophage response. Unexpectedly, there was no trend in the number of lymphocytes. A small study was also made of burn wound samples that had been obtained at post-mortem examination of subjects that died in a fire or up to 77 days after injury from fire. This revealed a similar trend of neutrophil and macrophage accumulation. Additionally,it appeared that an increase in the number of lymphocytes occurred late, from 35 days. In conclusion: If neutrophile predominate, the wound is probably less than a couple of days old. When macrophages are abundant the wound is probably a few days to weeks old. However, as expected from the review of the literature, the inflammatory cell infiltrate may be low or absent in burn wounds, which can render determination of the age of burn wounds difficult.	en_US
dc.publisher	Chiltern Publishing	en_US
dc.relation.ispartof	Medicine Science And The Law	en_US
dc.relation.isbasedon	0	en_US
dc.title	Using the inflammatory cell infiltrate to estimate the age of human burn wounds A review and immunohistochemical study	en_US
dc.type	Journal article
utslib.citation.volume	46	en_US
utslib.citation.number	2	en_US
utslib.location	Beaconsfield, UK	en_US
utslib.citation.startpage	115	en_US
utslib.citation.endpage	126	en_US
utslib.identifier.org	Chemistry, Materials and Forensic Sciences	en_US
utslib.copyright.status	closed_access

Abstract:

The burn wound healing process, which is reviewed in this paper, has features that differ from the healing of incised cutaneous wounds. This study used immunohistochemical staining and cell counting to examine the inflammatory cell response in biopsy samples of burn wounds from live human subjects obtained at six hours until 23 days after injury in order to determine how the age of a burn could be estimated. Acute inflammatory cells predominated in samples taken six hours to two days after injury. However, neutrophils were often minimal in early samples or could be present late. Elevated numbers of macrophages tended to be encountered from days 2 to 20, but it was not uncommon to observe a minimal or absent macrophage response. Unexpectedly, there was no trend in the number of lymphocytes. A small study was also made of burn wound samples that had been obtained at post-mortem examination of subjects that died in a fire or up to 77 days after injury from fire. This revealed a similar trend of neutrophil and macrophage accumulation. Additionally,it appeared that an increase in the number of lymphocytes occurred late, from 35 days. In conclusion: If neutrophile predominate, the wound is probably less than a couple of days old. When macrophages are abundant the wound is probably a few days to weeks old. However, as expected from the review of the literature, the inflammatory cell infiltrate may be low or absent in burn wounds, which can render determination of the age of burn wounds difficult.

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http://hdl.handle.net/10453/945