Time-resolved and temperature tuneable measurements of fluorescent intensity using a smartphone fluorimeter

Publication Type:
Journal Article
Citation:
Analyst, 2017, 142 (11), pp. 1953 - 1961
Issue Date:
2017-06-07
Full metadata record
Files in This Item:
Filename Description Size
Journal article.pdfSubmitted Version2.31 MB
Adobe PDF
© The Royal Society of Chemistry 2017. A smartphone fluorimeter capable of time-based fluorescence intensity measurements at various temperatures is reported. Excitation is provided by an integrated UV LED (λex= 370 nm) and detection obtained using the in-built CMOS camera. A Peltier is integrated to allow measurements of the intensity over T = 10 to 40 °C. All components are controlled using a smartphone battery powered Arduino microcontroller and a customised Android application that allows sequential fluorescence imaging and quantification every δt = 4 seconds. The temperature dependence of fluorescence intensity for four emitters (rhodamine B, rhodamine 6G, 5,10,15,20-tetraphenylporphyrin and 6-(1,4,8,11-tetraazacyclotetradecane)2-ethyl-naphthalimide) are characterised. The normalised fluorescence intensity over time of the latter chemosensor dye complex in the presence of Zn2+is observed to accelerate with an increasing rate constant, k = 1.94 min-1at T = 15 °C and k = 3.64 min-1at T = 30 °C, approaching a factor of ∼2 with only a change in temperature of ΔT = 15 °C. Thermally tuning these twist and bend associated rates to optimise sensor approaches and device applications is proposed.
Please use this identifier to cite or link to this item: