Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices

Ramalingam, N; Warkiani, ME; Hai-Qing Gong, T

Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices

Ramalingam, N Warkiani, ME

Hai-Qing Gong, T

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Publication Type:: Journal Article
Citation:: Biomicrofluidics, 2017, 11 (3)
Issue Date:: 2017-05-01

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Field	Value	Language
dc.contributor.author	Ramalingam, N	en_US
dc.contributor.author	Warkiani, ME https://orcid.org/0000-0002-4184-1944	en_US
dc.contributor.author	Hai-Qing Gong, T	en_US
dc.date.issued	2017-05-01	en_US
dc.identifier.citation	Biomicrofluidics, 2017, 11 (3)	en_US
dc.identifier.issn	1932-1058	en_US
dc.identifier.uri	http://hdl.handle.net/10453/126777
dc.description.abstract	Bovine serum albumin (BSA) is widely used as an additive in polymerase chain reaction (PCR)-based microfluidic devices to passivate reactors and alleviate nucleic-acid amplification. BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates that both types of BSA are used in PCR-based microfluidic devices. Our study results reveal that the use of acetylated BSA in PCR micro-devices leads to differential inhibition of PCR, compared to non-acetylated BSA. This result is noticed for the first time, and the differential inhibition generally goes un-noticed, as compared to complete PCR inhibition.	en_US
dc.relation.ispartof	Biomicrofluidics	en_US
dc.relation.isbasedon	10.1063/1.4983692	en_US
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Nanoscience & Nanotechnology	en_US
dc.title	Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	11	en_US
utslib.for	0915 Interdisciplinary Engineering	en_US
utslib.for	1007 Nanotechnology	en_US
utslib.for	0203 Classical Physics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access	*
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	11	en_US

Abstract:

Bovine serum albumin (BSA) is widely used as an additive in polymerase chain reaction (PCR)-based microfluidic devices to passivate reactors and alleviate nucleic-acid amplification. BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates that both types of BSA are used in PCR-based microfluidic devices. Our study results reveal that the use of acetylated BSA in PCR micro-devices leads to differential inhibition of PCR, compared to non-acetylated BSA. This result is noticed for the first time, and the differential inhibition generally goes un-noticed, as compared to complete PCR inhibition.

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http://hdl.handle.net/10453/126777