Novel reagents for quantitative analysis of valiolamine in biological samples by high-performance liquid chromatography with pre-column UV derivatization

Elsevier Inc
Publication Type:
Journal Article
Talanta, 2010, 81 (4-5), pp. 1613 - 1618
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A rapid, lowcost, high sensitive and quantitative method to detect valiolamine in a medium for microbial culture, involving derivatization with a new labeling reagent, 4methoxybenzenesulfonyl fluoride (MOBSF), followed by reversephase highperformance liquid chromatography with ultraviolet (UV) detection with simple operation procedure. 4Methoxybenzenesulfonyl chloride (MOBSCl) and 2nitrobenzenesulfonyl chloride (NBSCl) were compared with MOBSF as novel reagents in this paper, and the MOBSF was chosen as the most suitable derivatization reagent. The column was thermostatic at 35 C, the mobile phase flowrate was 1.0 mL/min and the detection wavelength was 240 nm. For a biological sample, the separation of the derivatives was achieved using a gradient mobile system. The elution program is 88% phosphate buffer (50mM; pH= 3.0) and 12% methanol for 23 min, then 70% of phosphate buffer and 30% methanol for another 15 min and finally 88% of phosphate buffer and 12% of methanol for 5 min to reequilibrate the column. The optimized conditions of the derivatization were as follows: derivatization reaction temperature 30 C; derivatization reaction pH value 11.0, reaction time 10 min and MOBSF concentration higher than 1.5mg/mL for standard solutions and higher than 5.0mg/mL for the biological sample. Calibration curves were linear in the range of 0.05025mg/mL for the standard solutions and 1.075mg/mL for the biological sample. The sensitive analytical method is helpful to control the biotechnological process of voglibose production and product quality control.
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