High-throughput sorting of eggs for synchronization of: C. elegans in a microfluidic spiral chip

Sofela, S; Sahloul, S; Rafeie, M; Kwon, T; Han, J; Warkiani, ME; Song, YA

High-throughput sorting of eggs for synchronization of: C. elegans in a microfluidic spiral chip

Sofela, S Sahloul, S Rafeie, M Kwon, T Han, J Warkiani, ME

Song, YA

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Publication Type:: Journal Article
Citation:: Lab on a Chip, 2018, 18 (4), pp. 679 - 687
Issue Date:: 2018-02-21

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Field	Value	Language
dc.contributor.author	Sofela, S	en_US
dc.contributor.author	Sahloul, S	en_US
dc.contributor.author	Rafeie, M	en_US
dc.contributor.author	Kwon, T	en_US
dc.contributor.author	Han, J	en_US
dc.contributor.author	Warkiani, ME https://orcid.org/0000-0002-4184-1944	en_US
dc.contributor.author	Song, YA	en_US
dc.date.issued	2018-02-21	en_US
dc.identifier.citation	Lab on a Chip, 2018, 18 (4), pp. 679 - 687	en_US
dc.identifier.issn	1473-0197	en_US
dc.identifier.uri	http://hdl.handle.net/10453/132773
dc.description.abstract	© 2018 The Royal Society of Chemistry. In this study, we report the use of a high-throughput microfluidic spiral chip to screen out eggs from a mixed age nematode population, which can subsequently be cultured to a desired developmental stage. For the sorting of a mixture containing three different developmental stages, eggs, L1 and L4, we utilized a microfluidic spiral chip with a trapezoidal channel to obtain a sorting efficiency of above 97% and a sample purity (SP) of above 80% for eggs at different flow rates up to 10 mL min-1. The result demonstrated a cost-effective, simple, and highly efficient method for synchronizing C. elegans at a high throughput (∼4200 organisms per min at 6 mL min-1), while eliminating challenges such as clogging and non-reusability of membrane-based filtration. Due to its simplicity, our method can be easily adopted in the C. elegans research community.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DP170101773
dc.relation.ispartof	Lab on a Chip	en_US
dc.relation.isbasedon	10.1039/c7lc00998d	en_US
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Caenorhabditis elegans	en_US
dc.subject.mesh	Microfluidic Analytical Techniques	en_US
dc.subject.mesh	Eggs	en_US
dc.subject.mesh	Lab-On-A-Chip Devices	en_US
dc.subject.mesh	High-Throughput Screening Assays	en_US
dc.title	High-throughput sorting of eggs for synchronization of: C. elegans in a microfluidic spiral chip	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	18	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	09 Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access	*
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	18	en_US

Abstract:

© 2018 The Royal Society of Chemistry. In this study, we report the use of a high-throughput microfluidic spiral chip to screen out eggs from a mixed age nematode population, which can subsequently be cultured to a desired developmental stage. For the sorting of a mixture containing three different developmental stages, eggs, L1 and L4, we utilized a microfluidic spiral chip with a trapezoidal channel to obtain a sorting efficiency of above 97% and a sample purity (SP) of above 80% for eggs at different flow rates up to 10 mL min-1. The result demonstrated a cost-effective, simple, and highly efficient method for synchronizing C. elegans at a high throughput (∼4200 organisms per min at 6 mL min-1), while eliminating challenges such as clogging and non-reusability of membrane-based filtration. Due to its simplicity, our method can be easily adopted in the C. elegans research community.

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http://hdl.handle.net/10453/132773