A simple coating method of PDMS microchip with PTFE for synthesis of dexamethasone-encapsulated PLGA nanoparticles

Mahmoodi, Z; Mohammadnejad, J; Razavi Bazaz, S; Abouei Mehrizi, A; Ghiass, MA; Saidijam, M; Dinarvand, R; Ebrahimi Warkiani, M; Soleimani, M

A simple coating method of PDMS microchip with PTFE for synthesis of dexamethasone-encapsulated PLGA nanoparticles

Mahmoodi, Z Mohammadnejad, J Razavi Bazaz, S

Abouei Mehrizi, A Ghiass, MA Saidijam, M Dinarvand, R Ebrahimi Warkiani, M

Soleimani, M

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Publication Type:: Journal Article
Citation:: Drug Delivery and Translational Research, 2019, 9 (3), pp. 707 - 720
Issue Date:: 2019-06-15

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Field	Value	Language
dc.contributor.author	Mahmoodi, Z	en_US
dc.contributor.author	Mohammadnejad, J	en_US
dc.contributor.author	Razavi Bazaz, S https://orcid.org/0000-0002-6419-3361	en_US
dc.contributor.author	Abouei Mehrizi, A	en_US
dc.contributor.author	Ghiass, MA	en_US
dc.contributor.author	Saidijam, M	en_US
dc.contributor.author	Dinarvand, R	en_US
dc.contributor.author	Ebrahimi Warkiani, M https://orcid.org/0000-0002-4184-1944	en_US
dc.contributor.author	Soleimani, M	en_US
dc.date.issued	2019-06-15	en_US
dc.identifier.citation	Drug Delivery and Translational Research, 2019, 9 (3), pp. 707 - 720	en_US
dc.identifier.issn	2190-393X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/134811
dc.description.abstract	© 2019, Controlled Release Society. Dexamethasone is a widely used drug in medical and biological applications. Since the systematic and controllable release of this drug is of significant importance, encapsulation of this anti-inflammatory drug in poly(lactic-co-glycolic acid) (PLGA) nanoparticles can minimize uncontrolled issues. As dexamethasone-encapsulated PLGA nanoparticles are synthesized in the presence of organic solvents, poly(dimethylsiloxane) (PDMS)-based microchannels collapse due to the swelling problem. In present study, PTFE nanoparticles were used for the surface modification of the microchannels to prevent absorption and adhesion of solvents into the microchannels’ wall. The contact angle analysis of microchips after coating showed that the surface of microchannels bear the superhydrophobicity feature (140.30°) and SEM images revealed that PTFE covered the surface of PDMS, favorably. Then, the prepared microchip was tested for the synthesis of dexamethasone-loaded nanoparticles. SEM and atomic force microscopy (AFM) images of the synthesized nanoparticles represented that there was not any evidence of adhesion or absorption of nanoparticles. Furthermore, the monodispersity of nanoparticles was discernible. As AFM results revealed, the average diameters of 47, 63, and 82 nm were achieved for flow ratios of 0.01, 0.05, and 0.1, respectively. To evaluate the drug efficiency, cumulative release and encapsulation efficiency were analyzed which showed much more efficiency than the synthesized nanoparticles in the bulk mode. In addition, MTT test revealed that nanoparticles could be considered as a non-toxic material. Since the synthesis of drug-loaded nanoparticles is ubiquitous in laboratory experiments, the approach presented in this study can render more versatility in this regard.	en_US
dc.relation.ispartof	Drug Delivery and Translational Research	en_US
dc.relation.isbasedon	10.1007/s13346-019-00636-z	en_US
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.mesh	Cells, Cultured	en_US
dc.subject.mesh	Mesenchymal Stem Cells	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Dimethylpolysiloxanes	en_US
dc.subject.mesh	Dexamethasone	en_US
dc.subject.mesh	Polytetrafluoroethylene	en_US
dc.subject.mesh	Anti-Inflammatory Agents	en_US
dc.subject.mesh	Cell Survival	en_US
dc.subject.mesh	Nanoparticles	en_US
dc.subject.mesh	Lab-On-A-Chip Devices	en_US
dc.subject.mesh	Polylactic Acid-Polyglycolic Acid Copolymer	en_US
dc.title	A simple coating method of PDMS microchip with PTFE for synthesis of dexamethasone-encapsulated PLGA nanoparticles	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	9	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access	*
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

© 2019, Controlled Release Society. Dexamethasone is a widely used drug in medical and biological applications. Since the systematic and controllable release of this drug is of significant importance, encapsulation of this anti-inflammatory drug in poly(lactic-co-glycolic acid) (PLGA) nanoparticles can minimize uncontrolled issues. As dexamethasone-encapsulated PLGA nanoparticles are synthesized in the presence of organic solvents, poly(dimethylsiloxane) (PDMS)-based microchannels collapse due to the swelling problem. In present study, PTFE nanoparticles were used for the surface modification of the microchannels to prevent absorption and adhesion of solvents into the microchannels’ wall. The contact angle analysis of microchips after coating showed that the surface of microchannels bear the superhydrophobicity feature (140.30°) and SEM images revealed that PTFE covered the surface of PDMS, favorably. Then, the prepared microchip was tested for the synthesis of dexamethasone-loaded nanoparticles. SEM and atomic force microscopy (AFM) images of the synthesized nanoparticles represented that there was not any evidence of adhesion or absorption of nanoparticles. Furthermore, the monodispersity of nanoparticles was discernible. As AFM results revealed, the average diameters of 47, 63, and 82 nm were achieved for flow ratios of 0.01, 0.05, and 0.1, respectively. To evaluate the drug efficiency, cumulative release and encapsulation efficiency were analyzed which showed much more efficiency than the synthesized nanoparticles in the bulk mode. In addition, MTT test revealed that nanoparticles could be considered as a non-toxic material. Since the synthesis of drug-loaded nanoparticles is ubiquitous in laboratory experiments, the approach presented in this study can render more versatility in this regard.

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http://hdl.handle.net/10453/134811