Validation of a Vasculogenesis Microfluidic Model for Radiobiological Studies of the Human Microvasculature

Guo, Z; Yang, CT; Maritz, MF; Wu, H; Wilson, P; Warkiani, ME; Chien, CC; Kempson, I; Aref, AR; Thierry, B

Validation of a Vasculogenesis Microfluidic Model for Radiobiological Studies of the Human Microvasculature

Guo, Z Yang, CT Maritz, MF Wu, H Wilson, P Warkiani, ME

Chien, CC Kempson, I Aref, AR Thierry, B

Permalink

Publication Type:: Journal Article
Citation:: Advanced Materials Technologies, 2019, 4 (4)
Issue Date:: 2019-04-01

Closed Access

	Filename	Description	Size
	Guo_et_al-2019-Advanced_Materials_Technologies.pdf	Published Version	2.76 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Guo, Z	en_US
dc.contributor.author	Yang, CT	en_US
dc.contributor.author	Maritz, MF	en_US
dc.contributor.author	Wu, H	en_US
dc.contributor.author	Wilson, P	en_US
dc.contributor.author	Warkiani, ME https://orcid.org/0000-0002-4184-1944	en_US
dc.contributor.author	Chien, CC	en_US
dc.contributor.author	Kempson, I	en_US
dc.contributor.author	Aref, AR	en_US
dc.contributor.author	Thierry, B	en_US
dc.date.issued	2019-04-01	en_US
dc.identifier.citation	Advanced Materials Technologies, 2019, 4 (4)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/139174
dc.description.abstract	© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim The therapeutic ratio of radiotherapy is limited by acute or chronic side effects with often severe consequences to patients. The microvasculature is a central player involved in both tumor responses and healthy tissue/organ radiological injuries. However, current preclinical vascular models based on 2D culture offer only limited radiobiological insight due to their failure in recapitulating the 3D nature experienced by endothelial cells within the human microvasculature. To address this issue, the use of a 3D microvasculature-on-a-chip microfluidic technology is demonstrated in radiobiological studies. Within this vasculogenesis model a perfusable network that structurally mimics the human microvasculature is formed and the biological response to ionizing radiation including cellular apoptosis, vessel tight adherens junction breakage, DNA double strand break, and repair is systematically investigated. In comparison to cells grown in a 2D environment, human umbilical vein endothelial cells in the 3D microvasculature-on-a-chip displays significant differences in biological responses, especially at high X-ray dose. This data confirms the feasibility of using microvascular-on-a-chip models for radiobiological studies. Such vasculogenesis models have strong potential to yield more accurate prediction of healthy tissue responses to ionizing radiation as well as to guide the development of risk-reducing strategies to prevent radiation-induced acute and long-term side-effects.	en_US
dc.relation.ispartof	Advanced Materials Technologies	en_US
dc.relation.isbasedon	10.1002/admt.201800726	en_US
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Validation of a Vasculogenesis Microfluidic Model for Radiobiological Studies of the Human Microvasculature	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	1112 Oncology and Carcinogenesis	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access	*
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	4	en_US

Abstract:

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim The therapeutic ratio of radiotherapy is limited by acute or chronic side effects with often severe consequences to patients. The microvasculature is a central player involved in both tumor responses and healthy tissue/organ radiological injuries. However, current preclinical vascular models based on 2D culture offer only limited radiobiological insight due to their failure in recapitulating the 3D nature experienced by endothelial cells within the human microvasculature. To address this issue, the use of a 3D microvasculature-on-a-chip microfluidic technology is demonstrated in radiobiological studies. Within this vasculogenesis model a perfusable network that structurally mimics the human microvasculature is formed and the biological response to ionizing radiation including cellular apoptosis, vessel tight adherens junction breakage, DNA double strand break, and repair is systematically investigated. In comparison to cells grown in a 2D environment, human umbilical vein endothelial cells in the 3D microvasculature-on-a-chip displays significant differences in biological responses, especially at high X-ray dose. This data confirms the feasibility of using microvascular-on-a-chip models for radiobiological studies. Such vasculogenesis models have strong potential to yield more accurate prediction of healthy tissue responses to ionizing radiation as well as to guide the development of risk-reducing strategies to prevent radiation-induced acute and long-term side-effects.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/139174