Fabrication of graphene-silver/polyurethane nanofibrous scaffolds for cardiac tissue engineering

Nazari, H; Azadi, S; Hatamie, S; Zomorrod, MS; Ashtari, K; Soleimani, M; Hosseinzadeh, S

Fabrication of graphene-silver/polyurethane nanofibrous scaffolds for cardiac tissue engineering

Nazari, H Azadi, S Hatamie, S Zomorrod, MS Ashtari, K Soleimani, M Hosseinzadeh, S

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Publication Type:: Journal Article
Citation:: Polymers for Advanced Technologies, 2019, 30 (8), pp. 2086 - 2099
Issue Date:: 2019-08-01

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Field	Value	Language
dc.contributor.author	Nazari, H	en_US
dc.contributor.author	Azadi, S	en_US
dc.contributor.author	Hatamie, S	en_US
dc.contributor.author	Zomorrod, MS	en_US
dc.contributor.author	Ashtari, K	en_US
dc.contributor.author	Soleimani, M	en_US
dc.contributor.author	Hosseinzadeh, S	en_US
dc.date.issued	2019-08-01	en_US
dc.identifier.citation	Polymers for Advanced Technologies, 2019, 30 (8), pp. 2086 - 2099	en_US
dc.identifier.issn	1042-7147	en_US
dc.identifier.uri	http://hdl.handle.net/10453/139222
dc.description.abstract	© 2019 John Wiley & Sons, Ltd. The graphene-based nanocomposites are considered as great candidates for enhancing electrical and mechanical properties of nonconductive scaffolds in cardiac tissue engineering. In this study, reduced graphene oxide-silver (rGO-Ag) nanocomposites (1 and 2 wt%) were synthesized and incorporated into polyurethane (PU) nanofibers via electrospinning technique. Next, the human cardiac progenitor cells (hCPCs) were seed on these scaffolds for in vitro studies. The rGO-Ag nanocomposites were studied by X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscope (TEM). After incorporation of rGO-Ag into PU nanofibers, the related characterizations were carried out including scanning electron microscope (SEM), TEM, water contact angle, and mechanical properties. Furthermore, PU and PU/nanocomposites scaffolds were used for in vitro studies, wherein hCPCs showed good cytocompatibility via 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and considerable attachment on the scaffold using SEM studies. Real-time polymerase chain reaction (PCR) and immunostaining studies confirmed the upregulation of cardiac specific genes including GATA-4, T-box 18 (TBX 18), cardiac troponin T (cTnT), and alpha-myosin heavy chain (α-MHC) in the PU/rGO-Ag scaffolds in comparison with neat PU ones. Therefore, these nanofibrous rGO-Ag–reinforced PU scaffolds can be considered as suitable candidates in cardiac tissue engineering.	en_US
dc.relation.ispartof	Polymers for Advanced Technologies	en_US
dc.relation.isbasedon	10.1002/pat.4641	en_US
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Polymers	en_US
dc.title	Fabrication of graphene-silver/polyurethane nanofibrous scaffolds for cardiac tissue engineering	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	30	en_US
utslib.for	1007 Nanotechnology	en_US
utslib.for	0303 Macromolecular and Materials Chemistry	en_US
utslib.for	0912 Materials Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	30	en_US

Abstract:

© 2019 John Wiley & Sons, Ltd. The graphene-based nanocomposites are considered as great candidates for enhancing electrical and mechanical properties of nonconductive scaffolds in cardiac tissue engineering. In this study, reduced graphene oxide-silver (rGO-Ag) nanocomposites (1 and 2 wt%) were synthesized and incorporated into polyurethane (PU) nanofibers via electrospinning technique. Next, the human cardiac progenitor cells (hCPCs) were seed on these scaffolds for in vitro studies. The rGO-Ag nanocomposites were studied by X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscope (TEM). After incorporation of rGO-Ag into PU nanofibers, the related characterizations were carried out including scanning electron microscope (SEM), TEM, water contact angle, and mechanical properties. Furthermore, PU and PU/nanocomposites scaffolds were used for in vitro studies, wherein hCPCs showed good cytocompatibility via 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and considerable attachment on the scaffold using SEM studies. Real-time polymerase chain reaction (PCR) and immunostaining studies confirmed the upregulation of cardiac specific genes including GATA-4, T-box 18 (TBX 18), cardiac troponin T (cTnT), and alpha-myosin heavy chain (α-MHC) in the PU/rGO-Ag scaffolds in comparison with neat PU ones. Therefore, these nanofibrous rGO-Ag–reinforced PU scaffolds can be considered as suitable candidates in cardiac tissue engineering.

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http://hdl.handle.net/10453/139222