Simulating Inflammation in a Wound Microenvironment Using a Dermal Wound-on-a-Chip Model

Biglari, S; Le, TYL; Tan, RP; Wise, SG; Zambon, A; Codolo, G; De Bernard, M; Warkiani, M; Schindeler, A; Naficy, S; Valtchev, P; Khademhosseini, A; Dehghani, F

Simulating Inflammation in a Wound Microenvironment Using a Dermal Wound-on-a-Chip Model

Biglari, S Le, TYL Tan, RP Wise, SG Zambon, A Codolo, G De Bernard, M Warkiani, M

Schindeler, A Naficy, S Valtchev, P Khademhosseini, A Dehghani, F

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Publication Type:: Journal Article
Citation:: Advanced Healthcare Materials, 2019, 8 (1)
Issue Date:: 2019-01-10

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Field	Value	Language
dc.contributor.author	Biglari, S	en_US
dc.contributor.author	Le, TYL	en_US
dc.contributor.author	Tan, RP	en_US
dc.contributor.author	Wise, SG	en_US
dc.contributor.author	Zambon, A	en_US
dc.contributor.author	Codolo, G	en_US
dc.contributor.author	De Bernard, M	en_US
dc.contributor.author	Warkiani, M https://orcid.org/0000-0002-4184-1944	en_US
dc.contributor.author	Schindeler, A	en_US
dc.contributor.author	Naficy, S	en_US
dc.contributor.author	Valtchev, P	en_US
dc.contributor.author	Khademhosseini, A	en_US
dc.contributor.author	Dehghani, F	en_US
dc.date.accessioned	2020-04-05T23:04:47Z
dc.date.available	2020-04-05T23:04:47Z
dc.date.issued	2019-01-10	en_US
dc.identifier.citation	Advanced Healthcare Materials, 2019, 8 (1)	en_US
dc.identifier.issn	2192-2640	en_US
dc.identifier.uri	http://hdl.handle.net/10453/139804
dc.description.abstract	© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Considerable progress has been made in the field of microfluidics to develop complex systems for modeling human skin and dermal wound healing processes. While microfluidic models have attempted to integrate multiple cell types and/or 3D culture systems, to date they have lacked some elements needed to fully represent dermal wound healing. This paper describes a cost-effective, multicellular microfluidic system that mimics the paracrine component of early inflammation close to normal wound healing. Collagen and Matrigel are tested as materials for coating and adhesion of dermal fibroblasts and human umbilical vein endothelial cells (HUVECs). The wound-on-chip model consists of three interconnecting channels and is able to simulate wound inflammation by adding tumor necrosis factor alpha (TNF-α) or by triculturing with macrophages. Both the approaches significantly increase IL-1β, IL-6, IL-8 in the supernatant (p < 0.05), and increases in cytokine levels are attenuated by cotreatment with an anti-inflammatory agent, Dexamethasone. Incorporation of M1 and M2 macrophages cocultured with fibroblasts and HUVECs leads to a stimulation of cytokine production as well as vascular structure formation, particularly with M2 macrophages. In summary, this wound-on-chip system can be used to model the paracrine component of the early inflammatory phase of wound healing and has the potential for the screening of anti-inflammatory compounds.	en_US
dc.relation.ispartof	Advanced Healthcare Materials	en_US
dc.relation.isbasedon	10.1002/adhm.201801307	en_US
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.mesh	Cell Culture Techniques	en_US
dc.subject.mesh	Cell Differentiation	en_US
dc.subject.mesh	Cellular Microenvironment	en_US
dc.subject.mesh	Cytokines	en_US
dc.subject.mesh	Dermis	en_US
dc.subject.mesh	Dexamethasone	en_US
dc.subject.mesh	Human Umbilical Vein Endothelial Cells	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Inflammation	en_US
dc.subject.mesh	Inflammation Mediators	en_US
dc.subject.mesh	Lab-On-A-Chip Devices	en_US
dc.subject.mesh	Macrophages	en_US
dc.subject.mesh	Models, Biological	en_US
dc.subject.mesh	Myofibroblasts	en_US
dc.subject.mesh	Neovascularization, Physiologic	en_US
dc.subject.mesh	Tumor Necrosis Factor-alpha	en_US
dc.subject.mesh	Wound Healing	en_US
dc.title	Simulating Inflammation in a Wound Microenvironment Using a Dermal Wound-on-a-Chip Model	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	8	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	1004 Medical Biotechnology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access	*
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Considerable progress has been made in the field of microfluidics to develop complex systems for modeling human skin and dermal wound healing processes. While microfluidic models have attempted to integrate multiple cell types and/or 3D culture systems, to date they have lacked some elements needed to fully represent dermal wound healing. This paper describes a cost-effective, multicellular microfluidic system that mimics the paracrine component of early inflammation close to normal wound healing. Collagen and Matrigel are tested as materials for coating and adhesion of dermal fibroblasts and human umbilical vein endothelial cells (HUVECs). The wound-on-chip model consists of three interconnecting channels and is able to simulate wound inflammation by adding tumor necrosis factor alpha (TNF-α) or by triculturing with macrophages. Both the approaches significantly increase IL-1β, IL-6, IL-8 in the supernatant (p < 0.05), and increases in cytokine levels are attenuated by cotreatment with an anti-inflammatory agent, Dexamethasone. Incorporation of M1 and M2 macrophages cocultured with fibroblasts and HUVECs leads to a stimulation of cytokine production as well as vascular structure formation, particularly with M2 macrophages. In summary, this wound-on-chip system can be used to model the paracrine component of the early inflammatory phase of wound healing and has the potential for the screening of anti-inflammatory compounds.

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http://hdl.handle.net/10453/139804