Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment.

Campbell, M; Chabria, M; Figtree, GA; Polonchuk, L; Gentile, C

Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment.

Campbell, M Chabria, M Figtree, GA Polonchuk, L Gentile, C

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Publisher:: Springer
Publication Type:: Chapter
Citation:: Stem Cell Niche, 2019, 2002, pp. 51-59
Issue Date:: 2019-01

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Field	Value	Language
dc.contributor.author	Campbell, M
dc.contributor.author	Chabria, M
dc.contributor.author	Figtree, GA
dc.contributor.author	Polonchuk, L
dc.contributor.author	Gentile, C https://orcid.org/0000-0002-3689-4275
dc.date.accessioned	2020-05-05T04:50:38Z
dc.date.available	2020-05-05T04:50:38Z
dc.date.issued	2019-01
dc.identifier.citation	Stem Cell Niche, 2019, 2002, pp. 51-59
dc.identifier.isbn	978-1-4939-9507-3
dc.identifier.uri	http://hdl.handle.net/10453/140487
dc.description.abstract	Our laboratory has recently developed a novel three-dimensional in vitro model of the human heart, which we call the vascularized cardiac spheroid (VCS). These better recapitulate the human heart's cellular and extracellular microenvironment compared to the existing in vitro models. To achieve this, human-induced pluripotent stem cell (iPSC)-derived cardiomyocytes, cardiac fibroblasts, and human coronary artery endothelial cells are co-cultured in hanging drop culture in ratios similar to those found in the human heart in vivo. The resulting three-dimensional cellular organization, extracellular matrix, and microvascular network formation throughout the VCS has been shown to mimic the one present in the human heart tissue. Therefore, VCSs offer a promising platform to study cardiac physiology, disease, and pharmacology, as well as bioengineering constructs to regenerate heart tissue.
dc.format	Print
dc.format.extent	15
dc.language	en
dc.publisher	Springer
dc.relation.ispartof	Stem Cell Niche
dc.relation.isbasedon	10.1007/7651_2018_187
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Fibroblasts
dc.subject.mesh	Myocytes, Cardiac
dc.subject.mesh	Humans
dc.subject.mesh	Imaging, Three-Dimensional
dc.subject.mesh	Coculture Techniques
dc.subject.mesh	Models, Biological
dc.subject.mesh	Bioengineering
dc.subject.mesh	Induced Pluripotent Stem Cells
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Fibroblasts
dc.subject.mesh	Myocytes, Cardiac
dc.subject.mesh	Humans
dc.subject.mesh	Imaging, Three-Dimensional
dc.subject.mesh	Coculture Techniques
dc.subject.mesh	Models, Biological
dc.subject.mesh	Bioengineering
dc.subject.mesh	Induced Pluripotent Stem Cells
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Bioengineering
dc.subject.mesh	Coculture Techniques
dc.subject.mesh	Extracellular Matrix
dc.subject.mesh	Fibroblasts
dc.subject.mesh	Humans
dc.subject.mesh	Imaging, Three-Dimensional
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Induced Pluripotent Stem Cells
dc.subject.mesh	Models, Biological
dc.subject.mesh	Myocytes, Cardiac
dc.subject.mesh	Spheroids, Cellular
dc.title	Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment.
dc.type	Chapter
utslib.citation.volume	2002
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-05-05T04:50:35Z
pubs.place-of-publication	Switzerland
pubs.publication-status	Published
pubs.volume	2002
utslib.start-page	51
dc.location	Switzerland

Abstract:

Our laboratory has recently developed a novel three-dimensional in vitro model of the human heart, which we call the vascularized cardiac spheroid (VCS). These better recapitulate the human heart's cellular and extracellular microenvironment compared to the existing in vitro models. To achieve this, human-induced pluripotent stem cell (iPSC)-derived cardiomyocytes, cardiac fibroblasts, and human coronary artery endothelial cells are co-cultured in hanging drop culture in ratios similar to those found in the human heart in vivo. The resulting three-dimensional cellular organization, extracellular matrix, and microvascular network formation throughout the VCS has been shown to mimic the one present in the human heart tissue. Therefore, VCSs offer a promising platform to study cardiac physiology, disease, and pharmacology, as well as bioengineering constructs to regenerate heart tissue.

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http://hdl.handle.net/10453/140487