Effect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
- Publication Type:
- Thesis
- Issue Date:
- 2020
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Microalgae are increasingly being used in recombinant protein production for a number of reasons including low cultivation costs, and the presence of post-translational modification mechanisms. There has been a lot of research on optimization of culture conditions in other expression systems like mammalian cell lines and plants to improve the yield of recombinant proteins. However, not much has been done on optimizing culture conditions to improve the yield of recombinant protein production from microalgae so far. The focus of this thesis was to investigate how different culture conditions affect recombinant protein production from nuclear transgenes in 𝘊. 𝘳𝘦𝘪𝘯𝘩𝘢𝘳𝘥𝘵𝘪𝘪, with the ultimate goal to find suitable optimized culture strategies to increase production without significant negative side effect on the growth and health of algal cultures. More particularly, this thesis investigated the effect of a culturing strategy using two different growth phases (i.e. biphasic growth) on recombinant protein production in 𝘊. 𝘳𝘦𝘪𝘯𝘩𝘢𝘳𝘥𝘵𝘪𝘪. The recombinant protein used in this study, interferon alpha 2 A (rIFN α2A) was chosen because of its high commercial value, anti- viral and anti-cancer properties.
Overall, my results suggest that biphasic growth (temperature regime and carbon source) can have significant effect on recombinant protein production in 𝘊. 𝘳𝘦𝘪𝘯𝘩𝘢𝘳𝘥𝘵𝘪𝘪 and should definitively be considered to improve application of microalgae as a commercially viable platform for recombinant protein production.
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