A Robust Intrinsically Green Fluorescent Poly(Amidoamine) Dendrimer for Imaging and Traceable Central Nervous System Delivery in Zebrafish.

Wang, G; Zhao, X; Wu, H; Lovejoy, DB; Zheng, M; Lee, A; Fu, L; Miao, K; An, Y; Sayyadi, N; Ding, K; Chung, RS; Lu, Y; Li, J; Morsch, M; Shi, B

A Robust Intrinsically Green Fluorescent Poly(Amidoamine) Dendrimer for Imaging and Traceable Central Nervous System Delivery in Zebrafish.

Wang, G Zhao, X Wu, H Lovejoy, DB Zheng, M Lee, A Fu, L Miao, K An, Y Sayyadi, N

Ding, K Chung, RS Lu, Y Li, J Morsch, M Shi, B

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Small, 2020, 16, (39), pp. 1-9
Issue Date:: 2020-09-02

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Field	Value	Language
dc.contributor.author	Wang, G
dc.contributor.author	Zhao, X
dc.contributor.author	Wu, H
dc.contributor.author	Lovejoy, DB
dc.contributor.author	Zheng, M
dc.contributor.author	Lee, A
dc.contributor.author	Fu, L
dc.contributor.author	Miao, K
dc.contributor.author	An, Y
dc.contributor.author	Sayyadi, N https://orcid.org/0000-0002-0044-2170
dc.contributor.author	Ding, K
dc.contributor.author	Chung, RS
dc.contributor.author	Lu, Y
dc.contributor.author	Li, J
dc.contributor.author	Morsch, M
dc.contributor.author	Shi, B
dc.date.accessioned	2020-10-20T01:01:33Z
dc.date.available	2020-10-20T01:01:33Z
dc.date.issued	2020-09-02
dc.identifier.citation	Small, 2020, 16, (39), pp. 1-9
dc.identifier.issn	1613-6810
dc.identifier.issn	1613-6829
dc.identifier.uri	http://hdl.handle.net/10453/143388
dc.description.abstract	Intrinsically fluorescent poly(amidoamine) dendrimers (IF-PAMAM) are an emerging class of versatile nanoplatforms for in vitro tracking and bio-imaging. However, limited tissue penetration of their fluorescence and interference due to auto-fluorescence arising from biological tissues limit its application in vivo. Herein, a green IF-PAMAM (FGP) dendrimer is reported and its biocompatibility, circulation, biodistribution and potential role for traceable central nervous system (CNS)-targeted delivery in zebrafish is evaluated, exploring various routes of administration. Key features of FGP include visible light excitation (488 nm), high fluorescence signal intensity, superior photostability and low interference from tissue auto-fluorescence. After intravenous injection, FGP shows excellent imaging and tracking performance in zebrafish. Further conjugating FGP with transferrin (FGP-Tf) significantly increases its penetration through the blood-brain barrier (BBB) and prolongs its circulation in the blood stream. When administering through local intratissue microinjection, including intracranial and intrathecal injection in zebrafish, both FGP and FGP-Tf exhibit excellent tissue diffusion and effective cellular uptake in the brain and spinal cord, respectively. This makes FGP/FGP-Tf attractive for in vivo tracing when transporting to the CNS is desired. The work addresses some of the major shortcomings in IF-PAMAM and provides a promising application of these probes in the development of drug delivery in the CNS.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Wiley
dc.relation.ispartof	Small
dc.relation.isbasedon	10.1002/smll.202003654
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Nanoscience & Nanotechnology
dc.title	A Robust Intrinsically Green Fluorescent Poly(Amidoamine) Dendrimer for Imaging and Traceable Central Nervous System Delivery in Zebrafish.
dc.type	Journal Article
utslib.citation.volume	16
utslib.location.activity	Germany
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-10-20T01:01:22Z
pubs.issue	39
pubs.publication-status	Published
pubs.volume	16
utslib.citation.issue	39

Abstract:

Intrinsically fluorescent poly(amidoamine) dendrimers (IF-PAMAM) are an emerging class of versatile nanoplatforms for in vitro tracking and bio-imaging. However, limited tissue penetration of their fluorescence and interference due to auto-fluorescence arising from biological tissues limit its application in vivo. Herein, a green IF-PAMAM (FGP) dendrimer is reported and its biocompatibility, circulation, biodistribution and potential role for traceable central nervous system (CNS)-targeted delivery in zebrafish is evaluated, exploring various routes of administration. Key features of FGP include visible light excitation (488 nm), high fluorescence signal intensity, superior photostability and low interference from tissue auto-fluorescence. After intravenous injection, FGP shows excellent imaging and tracking performance in zebrafish. Further conjugating FGP with transferrin (FGP-Tf) significantly increases its penetration through the blood-brain barrier (BBB) and prolongs its circulation in the blood stream. When administering through local intratissue microinjection, including intracranial and intrathecal injection in zebrafish, both FGP and FGP-Tf exhibit excellent tissue diffusion and effective cellular uptake in the brain and spinal cord, respectively. This makes FGP/FGP-Tf attractive for in vivo tracing when transporting to the CNS is desired. The work addresses some of the major shortcomings in IF-PAMAM and provides a promising application of these probes in the development of drug delivery in the CNS.

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http://hdl.handle.net/10453/143388