The ultrastructural organization of elastic fibers at the interface of the nucleus and annulus of the intervertebral disk.

Tavakoli, J; Diwan, AD; Tipper, JL

The ultrastructural organization of elastic fibers at the interface of the nucleus and annulus of the intervertebral disk.

Tavakoli, J

Diwan, AD Tipper, JL

Permalink

Publisher:: ELSEVIER SCI LTD
Publication Type:: Journal Article
Citation:: Acta biomaterialia, 2020, 114, pp. 323-332
Issue Date:: 2020-09

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

The embargo period expires on 30 Sep 2022

Adobe PDF

Download Accepted manuscriptAdobe PDF (1.83 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Tavakoli, J https://orcid.org/0000-0002-0696-6530
dc.contributor.author	Diwan, AD
dc.contributor.author	Tipper, JL
dc.date.accessioned	2020-11-12T04:34:49Z
dc.date.available	2020-07-09
dc.date.available	2020-11-12T04:34:49Z
dc.date.issued	2020-09
dc.identifier.citation	Acta biomaterialia, 2020, 114, pp. 323-332
dc.identifier.issn	1742-7061
dc.identifier.issn	1878-7568
dc.identifier.uri	http://hdl.handle.net/10453/143949
dc.description.abstract	There has been no study to describe the ultrastructural organization of elastic fibers at the interface of the nucleus pulposus and annulus fibrosus of the intervertebral disk (IVD), a region called the transition zone (TZ). A previously developed digestion technique was optimized to eliminate cells and non-elastin ECM components except for the elastic fibers from the anterolateral (AL) and posterolateral (PL) regions of the TZ in ovine IVDs. Not previously reported, the current study identified a complex elastic fiber network across the TZ for both AL and PL regions. In the AL region, this network consisted of major thick elastic fibers (≈ 1 µm) that were interconnected with delicate (< 200 nm) elastic fibers. While the same ultrastructural organization was observed in the PL region, interestingly the size of the elastic fibers was smaller (< 100 nm) compared to those that were located in the AL region. Quantitative analysis of the elastic fibers revealed significant differences in the size (p < 0.001) and the orientation of elastic fibers (p = 0.001) between the AL and PL regions, with a higher orientation and larger size of elastic fibers observed in the AL region. The gradual elimination of cells and non-elastin extracellular matrix components identified that elastic fibers in the TZ region in combination with the extracellular matrix created a honeycomb structure that was more compact at the AF interface compared to that located close to the NP. Three different symmetrically organized angles of rotation (0⁰ and ±90⁰) were detected for the honeycomb structure at both interfaces, and the structure was significantly orientated at the TZ-AF compared to the TZ-NP interface (p = 0.003).
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER SCI LTD
dc.relation.ispartof	Acta biomaterialia
dc.relation.isbasedon	10.1016/j.actbio.2020.07.021
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject.classification	Biomedical Engineering
dc.title	The ultrastructural organization of elastic fibers at the interface of the nucleus and annulus of the intervertebral disk.
dc.type	Journal Article
utslib.citation.volume	114
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
utslib.copyright.embargo	2022-09-30T00:00:00+1000Z
dc.date.updated	2020-11-12T04:34:34Z
pubs.publication-status	Published
pubs.volume	114

Abstract:

There has been no study to describe the ultrastructural organization of elastic fibers at the interface of the nucleus pulposus and annulus fibrosus of the intervertebral disk (IVD), a region called the transition zone (TZ). A previously developed digestion technique was optimized to eliminate cells and non-elastin ECM components except for the elastic fibers from the anterolateral (AL) and posterolateral (PL) regions of the TZ in ovine IVDs. Not previously reported, the current study identified a complex elastic fiber network across the TZ for both AL and PL regions. In the AL region, this network consisted of major thick elastic fibers (≈ 1 µm) that were interconnected with delicate (< 200 nm) elastic fibers. While the same ultrastructural organization was observed in the PL region, interestingly the size of the elastic fibers was smaller (< 100 nm) compared to those that were located in the AL region. Quantitative analysis of the elastic fibers revealed significant differences in the size (p < 0.001) and the orientation of elastic fibers (p = 0.001) between the AL and PL regions, with a higher orientation and larger size of elastic fibers observed in the AL region. The gradual elimination of cells and non-elastin extracellular matrix components identified that elastic fibers in the TZ region in combination with the extracellular matrix created a honeycomb structure that was more compact at the AF interface compared to that located close to the NP. Three different symmetrically organized angles of rotation (0⁰ and ±90⁰) were detected for the honeycomb structure at both interfaces, and the structure was significantly orientated at the TZ-AF compared to the TZ-NP interface (p = 0.003).

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/143949