Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration.

Hao, H; Niu, J; Xue, B; Su, QP; Liu, M; Yang, J; Qin, J; Zhao, S; Wu, C; Sun, Y

Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration.

Hao, H Niu, J Xue, B Su, QP Liu, M Yang, J Qin, J Zhao, S Wu, C Sun, Y

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: EMBO reports, 2020, 21, (3)
Issue Date:: 2020-03

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Field	Value	Language
dc.contributor.author	Hao, H
dc.contributor.author	Niu, J
dc.contributor.author	Xue, B
dc.contributor.author	Su, QP
dc.contributor.author	Liu, M
dc.contributor.author	Yang, J
dc.contributor.author	Qin, J
dc.contributor.author	Zhao, S
dc.contributor.author	Wu, C
dc.contributor.author	Sun, Y
dc.date.accessioned	2020-12-14T05:40:53Z
dc.date.available	2019-12-19
dc.date.available	2020-12-14T05:40:53Z
dc.date.issued	2020-03
dc.identifier.citation	EMBO reports, 2020, 21, (3)
dc.identifier.issn	1469-221X
dc.identifier.issn	1469-3178
dc.identifier.uri	http://hdl.handle.net/10453/144683
dc.description.abstract	Microtubules derived from the Golgi (Golgi MTs) have been implicated to play critical roles in persistent cell migration, but the underlying mechanisms remain elusive, partially due to the lack of direct observation of Golgi MT-dependent vesicular trafficking. Here, using super-resolution stochastic optical reconstruction microscopy (STORM), we discovered that post-Golgi cargos are more enriched on Golgi MTs and also surprisingly move much faster than on non-Golgi MTs. We found that, compared to non-Golgi MTs, Golgi MTs are morphologically more polarized toward the cell leading edge with significantly fewer inter-MT intersections. In addition, Golgi MTs are more stable and contain fewer lattice repair sites than non-Golgi MTs. Our STORM/live-cell imaging demonstrates that cargos frequently pause at the sites of both MT intersections and MT defects. Furthermore, by optogenetic maneuvering of cell direction, we demonstrate that Golgi MTs are essential for persistent cell migration but not for cells to change direction. Together, our study unveils the role of Golgi MTs in serving as a group of "fast tracks" for anterograde trafficking of post-Golgi cargos.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1177374
dc.relation	National Heart Foundation of Australia102592
dc.relation.ispartof	EMBO reports
dc.relation.isbasedon	10.15252/embr.201948385
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.title	Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration.
dc.type	Journal Article
utslib.citation.volume	21
utslib.location.activity	England
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-12-14T05:40:45Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	21
utslib.citation.issue	3

Abstract:

Microtubules derived from the Golgi (Golgi MTs) have been implicated to play critical roles in persistent cell migration, but the underlying mechanisms remain elusive, partially due to the lack of direct observation of Golgi MT-dependent vesicular trafficking. Here, using super-resolution stochastic optical reconstruction microscopy (STORM), we discovered that post-Golgi cargos are more enriched on Golgi MTs and also surprisingly move much faster than on non-Golgi MTs. We found that, compared to non-Golgi MTs, Golgi MTs are morphologically more polarized toward the cell leading edge with significantly fewer inter-MT intersections. In addition, Golgi MTs are more stable and contain fewer lattice repair sites than non-Golgi MTs. Our STORM/live-cell imaging demonstrates that cargos frequently pause at the sites of both MT intersections and MT defects. Furthermore, by optogenetic maneuvering of cell direction, we demonstrate that Golgi MTs are essential for persistent cell migration but not for cells to change direction. Together, our study unveils the role of Golgi MTs in serving as a group of "fast tracks" for anterograde trafficking of post-Golgi cargos.

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http://hdl.handle.net/10453/144683