Application of level-set method in simulation of normal and cancer cells deformability within a microfluidic device.

Mirzaaghaian, A; Ramiar, A; Ranjbar, AA; Warkiani, ME

Application of level-set method in simulation of normal and cancer cells deformability within a microfluidic device.

Mirzaaghaian, A Ramiar, A Ranjbar, AA Warkiani, ME

Permalink

Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Journal of biomechanics, 2020, 112, pp. 110066
Issue Date:: 2020-11

Closed Access

	Filename	Description	Size
	1-s2.0-S0021929020304905-main.pdf	Published version	2.34 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Mirzaaghaian, A
dc.contributor.author	Ramiar, A
dc.contributor.author	Ranjbar, AA
dc.contributor.author	Warkiani, ME
dc.date.accessioned	2020-12-27T07:34:46Z
dc.date.available	2020-09-17
dc.date.available	2020-12-27T07:34:46Z
dc.date.issued	2020-11
dc.identifier.citation	Journal of biomechanics, 2020, 112, pp. 110066
dc.identifier.issn	0021-9290
dc.identifier.issn	1873-2380
dc.identifier.uri	http://hdl.handle.net/10453/144960
dc.description.abstract	Application of microfluidic systems for the study of cellular behaviors has been a flourishing area of research in the past decade. In the process of probing cell biomechanics the passage of a cell through a narrow microchannel or a small pore has attracted much attention during the recent years. And the study of cellular deformability and transportability using these systems with enhanced resolution and accuracy has opened a new paradigm for high-throughput characterization of both healthy and diseased cell populations.Here we use the level-set method to explore the relationship between the transit time and mechanical properties of normal white blood cells (WBCs) and breast cancer epithelial cells (MCF7) under different microenvironmental parameters (i.e., pressure difference, cell size, effective cell surface tension, constriction size and taper angle) in a 2-D computational domain by considering the cell as a viscous drop. The novel biomechanical relations are obtained for each cell type by the Response Surface Method (RSM), relating microenvironmental parameters to the dimensionless entry time of the normal and cancer cells. Our results revealed that MCF7 cells show asignificantly different behavior (a bifurcating behavior when the pressure difference of inlet/outlet increases) in regards to the dimensionless entry time as a function of microchannel taper angle in comparison with the WBC. These results suggest that the microenvironmental parameters have a significant effect on the transportability of the cells and different cells have different behaviors in response to a specific microenvironmental parameter. Finally, it can be claimed that this method can be also utilized to distinguish between benign and cancerous cells or even to probe tumor heterogeneity toward high throughput cell cytometry.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier BV
dc.relation.ispartof	Journal of biomechanics
dc.relation.isbasedon	10.1016/j.jbiomech.2020.110066
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 0913 Mechanical Engineering, 1106 Human Movement and Sports Sciences
dc.subject.classification	Biomedical Engineering
dc.title	Application of level-set method in simulation of normal and cancer cells deformability within a microfluidic device.
dc.type	Journal Article
utslib.citation.volume	112
utslib.location.activity	United States
utslib.for	0903 Biomedical Engineering
utslib.for	0913 Mechanical Engineering
utslib.for	1106 Human Movement and Sports Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-12-27T07:34:29Z
pubs.publication-status	Published
pubs.volume	112

Abstract:

Application of microfluidic systems for the study of cellular behaviors has been a flourishing area of research in the past decade. In the process of probing cell biomechanics the passage of a cell through a narrow microchannel or a small pore has attracted much attention during the recent years. And the study of cellular deformability and transportability using these systems with enhanced resolution and accuracy has opened a new paradigm for high-throughput characterization of both healthy and diseased cell populations.Here we use the level-set method to explore the relationship between the transit time and mechanical properties of normal white blood cells (WBCs) and breast cancer epithelial cells (MCF7) under different microenvironmental parameters (i.e., pressure difference, cell size, effective cell surface tension, constriction size and taper angle) in a 2-D computational domain by considering the cell as a viscous drop. The novel biomechanical relations are obtained for each cell type by the Response Surface Method (RSM), relating microenvironmental parameters to the dimensionless entry time of the normal and cancer cells. Our results revealed that MCF7 cells show asignificantly different behavior (a bifurcating behavior when the pressure difference of inlet/outlet increases) in regards to the dimensionless entry time as a function of microchannel taper angle in comparison with the WBC. These results suggest that the microenvironmental parameters have a significant effect on the transportability of the cells and different cells have different behaviors in response to a specific microenvironmental parameter. Finally, it can be claimed that this method can be also utilized to distinguish between benign and cancerous cells or even to probe tumor heterogeneity toward high throughput cell cytometry.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/144960