PCR-free paper-based nanobiosensing platform for visual detection of telomerase activity via gold enhancement

Mahmoudi, T; Pirpour Tazehkand, A; Pourhassan-Moghaddam, M; Alizadeh-Ghodsi, M; Ding, L; Baradaran, B; Razavi Bazaz, S; Jin, D; Ebrahimi Warkiani, M

PCR-free paper-based nanobiosensing platform for visual detection of telomerase activity via gold enhancement

Mahmoudi, T Pirpour Tazehkand, A Pourhassan-Moghaddam, M Alizadeh-Ghodsi, M Ding, L Baradaran, B Razavi Bazaz, S

Jin, D

Ebrahimi Warkiani, M

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Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: Microchemical Journal, 2020, 154
Issue Date:: 2020-05-01

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Field	Value	Language
dc.contributor.author	Mahmoudi, T
dc.contributor.author	Pirpour Tazehkand, A
dc.contributor.author	Pourhassan-Moghaddam, M
dc.contributor.author	Alizadeh-Ghodsi, M
dc.contributor.author	Ding, L
dc.contributor.author	Baradaran, B
dc.contributor.author	Razavi Bazaz, S https://orcid.org/0000-0002-6419-3361
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.contributor.author	Ebrahimi Warkiani, M https://orcid.org/0000-0002-4184-1944
dc.date.accessioned	2021-01-12T03:36:19Z
dc.date.available	2021-01-12T03:36:19Z
dc.date.issued	2020-05-01
dc.identifier.citation	Microchemical Journal, 2020, 154
dc.identifier.issn	0026-265X
dc.identifier.issn	1095-9149
dc.identifier.uri	http://hdl.handle.net/10453/145324
dc.description.abstract	© 2020 Elsevier B.V. Telomerase activity has been demonstrated in a wide variety of most solid tumors and considered as a well-known cancer biomarker. The commonly utilized method for its detection is polymerase chain reaction (PCR)-based telomeric repeat amplification protocol (TRAP). However, the TRAP technique suffers from false-negative results caused by the failure of PCR step. Moreover, it requires advanced equipment with a tedious and time-consuming procedure. Herein, we presented a portable nitrocellulose paper-based nanobiosensing platform for ultrafast and equipment-free detection of telomerase activity based on a simple colorimetric assay that enabled naked-eye visualization of the color change in response to enzyme activity. In this platform, hybridization was initially performed between telomere complementary oligonucleotide immobilized on gold nanoparticles (GNPs) and telomerase elongated biotinylated probe. Thereafter, the assembly was attached on activated paper strip via avidin-biotin interaction. The signal amplification was carried out by enlargement of the attached GNPs on the paper strip, forming tightly compact rod-shaped submicron structures of gold representing a visual color formation. Thanks to significant sensitivity enhancement, the color change was occurred for down to 6 cells, which can be easily observed by the naked eye. Due to the desired aspects of the developed assay including PCR-free, low cost, simple, and high sensitivity, it can be used for evaluation of telomerase activity in cell extracts for future clinical applications. Furthermore, this design has the ability to be easily integrated into lab-on-chip devices for point-of-care telomerase sensing.
dc.language	English
dc.publisher	ELSEVIER
dc.relation	http://purl.org/au-research/grants/arc/DP170103704
dc.relation	http://purl.org/au-research/grants/arc/DP180103003
dc.relation	http://purl.org/au-research/grants/nhmrc/GNT1143377
dc.relation	http://purl.org/au-research/grants/nhmrc/1143377
dc.relation.ispartof	Microchemical Journal
dc.relation.isbasedon	10.1016/j.microc.2020.104594
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry
dc.subject.classification	Analytical Chemistry
dc.title	PCR-free paper-based nanobiosensing platform for visual detection of telomerase activity via gold enhancement
dc.type	Journal Article
utslib.citation.volume	154
utslib.for	0301 Analytical Chemistry
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2021-01-12T03:36:14Z
pubs.publication-status	Published
pubs.volume	154

Abstract:

© 2020 Elsevier B.V. Telomerase activity has been demonstrated in a wide variety of most solid tumors and considered as a well-known cancer biomarker. The commonly utilized method for its detection is polymerase chain reaction (PCR)-based telomeric repeat amplification protocol (TRAP). However, the TRAP technique suffers from false-negative results caused by the failure of PCR step. Moreover, it requires advanced equipment with a tedious and time-consuming procedure. Herein, we presented a portable nitrocellulose paper-based nanobiosensing platform for ultrafast and equipment-free detection of telomerase activity based on a simple colorimetric assay that enabled naked-eye visualization of the color change in response to enzyme activity. In this platform, hybridization was initially performed between telomere complementary oligonucleotide immobilized on gold nanoparticles (GNPs) and telomerase elongated biotinylated probe. Thereafter, the assembly was attached on activated paper strip via avidin-biotin interaction. The signal amplification was carried out by enlargement of the attached GNPs on the paper strip, forming tightly compact rod-shaped submicron structures of gold representing a visual color formation. Thanks to significant sensitivity enhancement, the color change was occurred for down to 6 cells, which can be easily observed by the naked eye. Due to the desired aspects of the developed assay including PCR-free, low cost, simple, and high sensitivity, it can be used for evaluation of telomerase activity in cell extracts for future clinical applications. Furthermore, this design has the ability to be easily integrated into lab-on-chip devices for point-of-care telomerase sensing.

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http://hdl.handle.net/10453/145324