Metal-Organic Framework-Enhanced ELISA Platform for Ultrasensitive Detection of PD-L1

Zhand, S; Razmjou, A; Azadi, S; Bazaz, SR; Shrestha, J; Jahromi, MAF; Warkiani, ME

Metal-Organic Framework-Enhanced ELISA Platform for Ultrasensitive Detection of PD-L1

Zhand, S Razmjou, A Azadi, S Bazaz, SR Shrestha, J Jahromi, MAF Warkiani, ME

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Publisher:: American Chemical Society (ACS)
Publication Type:: Journal Article
Citation:: ACS Applied Bio Materials, 2020, 3, (7), pp. 4148-4158
Issue Date:: 2020-07-20

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Field	Value	Language
dc.contributor.author	Zhand, S
dc.contributor.author	Razmjou, A
dc.contributor.author	Azadi, S
dc.contributor.author	Bazaz, SR
dc.contributor.author	Shrestha, J
dc.contributor.author	Jahromi, MAF
dc.contributor.author	Warkiani, ME
dc.date.accessioned	2021-03-01T21:09:30Z
dc.date.available	2021-03-01T21:09:30Z
dc.date.issued	2020-07-20
dc.identifier.citation	ACS Applied Bio Materials, 2020, 3, (7), pp. 4148-4158
dc.identifier.issn	2576-6422
dc.identifier.issn	2576-6422
dc.identifier.uri	http://hdl.handle.net/10453/146590
dc.description.abstract	© 2020 American Chemical Society. The programmed cell death ligand 1 (PD-L1) protein has emerged as a predictive cancer biomarker and sensitivity to immune checkpoint blockade-based cancer immunotherapies. Current technologies for the detection of protein-based biomarkers, including the enzyme-linked immunosorbent assay (ELISA), have limitations such as low sensitivity and limit of detection (LOD) in addition to degradation of antibodies in exposure to environmental changes such as temperature and pH. To address these issues, we have proposed a metal-organic framework (MOF)-based ELISA for the detection of the PD-L1. A protective coating based on Zeolitic Imidazolate Framework 8 (ZIF-8) MOF thin film and polydopamine-polyethylenimine (PDA-PEI) was introduced on an ELISA plate for the improvement of antibody immobilization. Sensitivity and LOD of the resulting platform were compared with a conventional ELISA kit, and the bioactivity of the antibody in the proposed immunoassay was investigated in response to various pH and temperature values. The LOD and sensitivity of the MOF-based PD-L1 ELISA were 225 and 15.12 times higher, respectively, compared with those of the commercial ELISA kit. The antibody@ZIF-8/PDA-PEI was stable up to 55 °C and the pH range 5-10. The proposed platform can provide sensitive detection for target proteins, in addition to being resistant to elevated temperature and pH. The proposed MOF-based ELISA has significant potential for the clinical and diagnostic studies.
dc.language	en
dc.publisher	American Chemical Society (ACS)
dc.relation	http://purl.org/au-research/grants/arc/DP170103704
dc.relation	http://purl.org/au-research/grants/arc/DP180103003
dc.relation	http://purl.org/au-research/grants/nhmrc/1143377
dc.relation.ispartof	ACS Applied Bio Materials
dc.relation.isbasedon	10.1021/acsabm.0c00227
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Metal-Organic Framework-Enhanced ELISA Platform for Ultrasensitive Detection of PD-L1
dc.type	Journal Article
utslib.citation.volume	3
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
utslib.copyright.status	open_access	*
dc.date.updated	2021-03-01T21:09:25Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	3
utslib.citation.issue	7

Abstract:

© 2020 American Chemical Society. The programmed cell death ligand 1 (PD-L1) protein has emerged as a predictive cancer biomarker and sensitivity to immune checkpoint blockade-based cancer immunotherapies. Current technologies for the detection of protein-based biomarkers, including the enzyme-linked immunosorbent assay (ELISA), have limitations such as low sensitivity and limit of detection (LOD) in addition to degradation of antibodies in exposure to environmental changes such as temperature and pH. To address these issues, we have proposed a metal-organic framework (MOF)-based ELISA for the detection of the PD-L1. A protective coating based on Zeolitic Imidazolate Framework 8 (ZIF-8) MOF thin film and polydopamine-polyethylenimine (PDA-PEI) was introduced on an ELISA plate for the improvement of antibody immobilization. Sensitivity and LOD of the resulting platform were compared with a conventional ELISA kit, and the bioactivity of the antibody in the proposed immunoassay was investigated in response to various pH and temperature values. The LOD and sensitivity of the MOF-based PD-L1 ELISA were 225 and 15.12 times higher, respectively, compared with those of the commercial ELISA kit. The antibody@ZIF-8/PDA-PEI was stable up to 55 °C and the pH range 5-10. The proposed platform can provide sensitive detection for target proteins, in addition to being resistant to elevated temperature and pH. The proposed MOF-based ELISA has significant potential for the clinical and diagnostic studies.

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http://hdl.handle.net/10453/146590