Modelling of red blood cell morphological and deformability changes during in-vitro storage

Geekiyanage, N; Sauret, E; Saha, S; Flower, R; Gu, YT

Modelling of red blood cell morphological and deformability changes during in-vitro storage

Geekiyanage, N Sauret, E Saha, S

Flower, R Gu, YT

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Applied Sciences (Switzerland), 2020, 10, (9)
Issue Date:: 2020-05-01

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Field	Value	Language
dc.contributor.author	Geekiyanage, N
dc.contributor.author	Sauret, E
dc.contributor.author	Saha, S https://orcid.org/0000-0002-9962-8919
dc.contributor.author	Flower, R
dc.contributor.author	Gu, YT
dc.date.accessioned	2021-03-07T07:36:48Z
dc.date.available	2021-03-07T07:36:48Z
dc.date.issued	2020-05-01
dc.identifier.citation	Applied Sciences (Switzerland), 2020, 10, (9)
dc.identifier.issn	1454-5101
dc.identifier.issn	2076-3417
dc.identifier.uri	http://hdl.handle.net/10453/146856
dc.description.abstract	© 2020 by the authors. Storage lesion is a critical issue facing transfusion treatments, and it adversely affects the quality and viability of stored red blood cells (RBCs). RBC deformability is a key indicator of cell health. Deformability measurements of each RBC unit are a key challenge in transfusion medicine research and clinical haematology. In this paper, a numerical study, inspired from the previous research for RBC deformability and morphology predictions, is conducted for the first time, to investigate the deformability and morphology characteristics of RBCs undergoing storage lesion. This study investigates the evolution of the cell shape factor, elongation index and membrane spicule details, where applicable, of discocyte, echinocyte I, echinocyte II, echinocyte III and sphero-echinocyte morphologies during 42 days of in-vitro storage at 4 °C in saline-adenine-glucose-mannitol (SAGM). Computer simulations were performed to investigate the influence of storage lesion-induced membrane structural defects on cell deformability and its recoverability during optical tweezers stretching deformations. The predicted morphology and deformability indicate decreasing quality and viability of stored RBCs undergoing storage lesion. The loss of membrane structural integrity due to the storage lesion further degrades the cell deformability and recoverability during mechanical deformations. This numerical approach provides a potential framework to study the RBC deformation characteristics under varying pathophysiological conditions for better diagnostics and treatments.
dc.language	English
dc.publisher	MDPI
dc.relation	http://purl.org/au-research/grants/arc/LP150100737
dc.relation.ispartof	Applied Sciences (Switzerland)
dc.relation.isbasedon	10.3390/app10093209
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Modelling of red blood cell morphological and deformability changes during in-vitro storage
dc.type	Journal Article
utslib.citation.volume	10
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Mechanical and Mechatronic Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2021-03-07T07:36:42Z
pubs.issue	9
pubs.publication-status	Published
pubs.volume	10
utslib.citation.issue	9

Abstract:

© 2020 by the authors. Storage lesion is a critical issue facing transfusion treatments, and it adversely affects the quality and viability of stored red blood cells (RBCs). RBC deformability is a key indicator of cell health. Deformability measurements of each RBC unit are a key challenge in transfusion medicine research and clinical haematology. In this paper, a numerical study, inspired from the previous research for RBC deformability and morphology predictions, is conducted for the first time, to investigate the deformability and morphology characteristics of RBCs undergoing storage lesion. This study investigates the evolution of the cell shape factor, elongation index and membrane spicule details, where applicable, of discocyte, echinocyte I, echinocyte II, echinocyte III and sphero-echinocyte morphologies during 42 days of in-vitro storage at 4 °C in saline-adenine-glucose-mannitol (SAGM). Computer simulations were performed to investigate the influence of storage lesion-induced membrane structural defects on cell deformability and its recoverability during optical tweezers stretching deformations. The predicted morphology and deformability indicate decreasing quality and viability of stored RBCs undergoing storage lesion. The loss of membrane structural integrity due to the storage lesion further degrades the cell deformability and recoverability during mechanical deformations. This numerical approach provides a potential framework to study the RBC deformation characteristics under varying pathophysiological conditions for better diagnostics and treatments.

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http://hdl.handle.net/10453/146856