Quantitatively Monitoring <i>In Situ</i> Mitochondrial Thermal Dynamics by Upconversion Nanoparticles.

Di, X; Wang, D; Zhou, J; Zhang, L; Stenzel, MH; Su, QP; Jin, D

Quantitatively Monitoring <i>In Situ</i> Mitochondrial Thermal Dynamics by Upconversion Nanoparticles.

Di, X Wang, D Zhou, J

Zhang, L Stenzel, MH Su, QP Jin, D

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: Nano letters, 2021, 21, (4), pp. 1651-1658
Issue Date:: 2021-02-06

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Field	Value	Language
dc.contributor.author	Di, X
dc.contributor.author	Wang, D
dc.contributor.author	Zhou, J https://orcid.org/0000-0002-0605-5745
dc.contributor.author	Zhang, L
dc.contributor.author	Stenzel, MH
dc.contributor.author	Su, QP
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.date.accessioned	2021-04-26T04:10:33Z
dc.date.available	2021-04-26T04:10:33Z
dc.date.issued	2021-02-06
dc.identifier.citation	Nano letters, 2021, 21, (4), pp. 1651-1658
dc.identifier.issn	1530-6984
dc.identifier.issn	1530-6992
dc.identifier.uri	http://hdl.handle.net/10453/148365
dc.description.abstract	Temperature dynamics reflect the physiological conditions of cells and organisms. Mitochondria regulate the temperature dynamics in living cells as they oxidize the respiratory substrates and synthesize ATP, with heat being released as a byproduct of active metabolism. Here, we report an upconversion nanoparticle-based thermometer that allows the <i>in situ</i> thermal dynamics monitoring of mitochondria in living cells. We demonstrate that the upconversion nanothermometers can efficiently target mitochondria, and the temperature-responsive feature is independent of probe concentration and medium conditions. The relative sensing sensitivity of 3.2% K<sup>-1</sup> in HeLa cells allows us to measure the mitochondrial temperature difference through the stimulations of high glucose, lipid, Ca<sup>2+</sup> shock, and the inhibitor of oxidative phosphorylation. Moreover, cells display distinct response time and thermodynamic profiles under different stimulations, which highlight the potential applications of this thermometer to study <i>in situ</i> vital processes related to mitochondrial metabolism pathways and interactions between organelles.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation	Department of Innovation, Industry, Science and Research (Previously known as DEST)VNSDSHWJ
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1177374
dc.relation	National Heart Foundation of Australia102592
dc.relation	http://purl.org/au-research/grants/arc/DP200101970
dc.relation	http://purl.org/au-research/grants/arc/FT130100517
dc.relation	http://purl.org/au-research/grants/arc/DE180100669
dc.relation.ispartof	Nano letters
dc.relation.isbasedon	10.1021/acs.nanolett.0c04281
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	Nanoscience & Nanotechnology
dc.title	Quantitatively Monitoring <i>In Situ</i> Mitochondrial Thermal Dynamics by Upconversion Nanoparticles.
dc.type	Journal Article
utslib.citation.volume	21
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access	*
dc.date.updated	2021-04-26T04:10:31Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	21
utslib.citation.issue	4

Abstract:

Temperature dynamics reflect the physiological conditions of cells and organisms. Mitochondria regulate the temperature dynamics in living cells as they oxidize the respiratory substrates and synthesize ATP, with heat being released as a byproduct of active metabolism. Here, we report an upconversion nanoparticle-based thermometer that allows the in situ thermal dynamics monitoring of mitochondria in living cells. We demonstrate that the upconversion nanothermometers can efficiently target mitochondria, and the temperature-responsive feature is independent of probe concentration and medium conditions. The relative sensing sensitivity of 3.2% K^-1 in HeLa cells allows us to measure the mitochondrial temperature difference through the stimulations of high glucose, lipid, Ca²⁺ shock, and the inhibitor of oxidative phosphorylation. Moreover, cells display distinct response time and thermodynamic profiles under different stimulations, which highlight the potential applications of this thermometer to study in situ vital processes related to mitochondrial metabolism pathways and interactions between organelles.

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http://hdl.handle.net/10453/148365