| Field |
Value |
Language |
|
dc.contributor.author |
Di, X |
|
|
dc.contributor.author |
Wang, D |
|
|
dc.contributor.author |
Zhou, J
https://orcid.org/0000-0002-0605-5745
|
|
|
dc.contributor.author |
Zhang, L |
|
|
dc.contributor.author |
Stenzel, M |
|
|
dc.contributor.author |
Su, QP |
|
|
dc.contributor.author |
Jin, D |
|
|
dc.date.accessioned |
2021-04-26T04:12:30Z |
|
|
dc.date.available |
2021-04-26T04:12:30Z |
|
|
dc.date.issued |
2020-11-29 |
|
|
dc.identifier.citation |
2020, pp. 2020.11.29.402818 |
|
|
dc.identifier.uri |
http://hdl.handle.net/10453/148367
|
|
|
dc.description.abstract |
<jats:title>Abstract</jats:title><jats:p>Temperature dynamics reflect the physiological conditions of cells and organisms. Mitochondria regulates temperature dynamics in living cells, as they oxidize the respiratory substrates and synthesize ATP, with heat being released as a by-product of active metabolism. Here, we report an upconversion nanoparticles based thermometer that allows <jats:italic>in situ</jats:italic> thermal dynamics monitoring of mitochondria in living cells. We demonstrate that the upconversion nanothermometers can efficiently target mitochondria and the temperature responsive feature is independent of probe concentration and medium conditions. The relative sensing sensitivity of 3.2% K<jats:sup>−1</jats:sup> in HeLa cells allows us to measure the mitochondrial temperature difference through the stimulations of high glucose, lipid, Ca<jats:sup>2+</jats:sup> shock and the inhibitor of oxidative phosphorylation. Moreover, cells display distinct response time and thermal dynamic profiles under different stimulations, which highlights the potential applications of this thermometer to study <jats:italic>in situ</jats:italic> vital processes related to mitochondrial metabolism pathways and interactions between organelles.</jats:p> |
|
|
dc.language |
en |
|
|
dc.relation |
http://purl.org/au-research/grants/nhmrc/APP1177374
|
|
|
dc.relation |
National Heart Foundation of Australia102592 |
|
|
dc.relation |
http://purl.org/au-research/grants/arc/DP200101970
|
|
|
dc.relation |
Department of Innovation, Industry, Science and Research (Previously known as DEST)VNSDSHWJ |
|
|
dc.relation |
http://purl.org/au-research/grants/arc/FT130100517
|
|
|
dc.relation |
http://purl.org/au-research/grants/arc/DE180100669
|
|
|
dc.relation |
http://purl.org/au-research/grants/nhmrc/GNT1177374
|
|
|
dc.relation |
http://purl.org/au-research/grants/nhmrc/1177374
|
|
|
dc.relation.isbasedon |
10.1101/2020.11.29.402818 |
|
|
dc.rights |
info:eu-repo/semantics/openAccess |
|
|
dc.title |
Quantitatively Monitoring in situ Mitochondrial Thermal Dynamics by Upconversion Nanoparticles |
|
|
dc.type |
Journal Article |
|
|
pubs.organisational-group |
/University of Technology Sydney |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Engineering and Information Technology |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Science |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering |
|
|
pubs.organisational-group |
/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices |
|
|
utslib.copyright.status |
open_access |
* |
|
dc.date.updated |
2021-04-26T04:12:29Z |
|
