The biological response to nanometre-sized polymer particles.

Liu, A; Richards, L; Bladen, CL; Ingham, E; Fisher, J; Tipper, JL

The biological response to nanometre-sized polymer particles.

Liu, A Richards, L Bladen, CL Ingham, E Fisher, J Tipper, JL

Permalink

Publisher:: ELSEVIER SCI LTD
Publication Type:: Journal Article
Citation:: Acta biomaterialia, 2015, 23, pp. 38-51
Issue Date:: 2015-09

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (4.34 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Liu, A
dc.contributor.author	Richards, L
dc.contributor.author	Bladen, CL
dc.contributor.author	Ingham, E
dc.contributor.author	Fisher, J
dc.contributor.author	Tipper, JL
dc.date.accessioned	2021-07-21T07:25:55Z
dc.date.available	2015-05-15
dc.date.available	2021-07-21T07:25:55Z
dc.date.issued	2015-09
dc.identifier.citation	Acta biomaterialia, 2015, 23, pp. 38-51
dc.identifier.issn	1742-7061
dc.identifier.issn	1878-7568
dc.identifier.uri	http://hdl.handle.net/10453/149906
dc.description.abstract	Recently, nanometre-sized UHMWPE particles generated from hip and knee replacements have been identified in vitro and in vivo. UHMWPE particles in the 0.1-1.0μm size range have been shown to be more biologically active than larger particles, provoking an inflammatory response implicated in late aseptic loosening of total joint replacements. The biological activity of nanometre-sized particles has not previously been studied. The biological response to clinically-relevant UHMWPE wear particles including nanometre-sized and micrometre-sized, along with polystyrene particles (FluoSpheres 20nm, 60nm, 200nm and 1.0μm), and nanometre-sized model polyethylene particles (Ceridust 3615®), was determined in terms of osteolytic cytokine release from primary human peripheral blood mononuclear cells (PBMNCs). Nanometre-sized UHMWPE wear particles, nanometre-sized Ceridust 3615® and 20nm FluoSpheres had no significant effect on TNF-α, IL-1β, IL-6 and IL-8 release from PBMNCs at a concentration of 100μm(3) particles per cell after 12 and 24h. The micrometre-size UHMWPE wear particles (0.1-1.0μm) and 60nm, 200nm and 1.0μm FluoSpheres caused significantly elevated osteolytic cytokine release from PBMNCs. These results indicated that particles below circa 50nm fail to activate PBMNCs and that particle size, composition and morphology played a crucial role in cytokine release by particle stimulated macrophages.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER SCI LTD
dc.relation.ispartof	Acta biomaterialia
dc.relation.isbasedon	10.1016/j.actbio.2015.05.016
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Leukocytes, Mononuclear
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Humans
dc.subject.mesh	Polyethylenes
dc.subject.mesh	Immunologic Factors
dc.subject.mesh	Cytokines
dc.subject.mesh	Materials Testing
dc.subject.mesh	Dose-Response Relationship, Drug
dc.subject.mesh	Particle Size
dc.subject.mesh	Adult
dc.subject.mesh	Middle Aged
dc.subject.mesh	Female
dc.subject.mesh	Male
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Young Adult
dc.subject.mesh	Adult
dc.subject.mesh	Cells, Cultured
dc.subject.mesh	Cytokines
dc.subject.mesh	Dose-Response Relationship, Drug
dc.subject.mesh	Female
dc.subject.mesh	Humans
dc.subject.mesh	Immunologic Factors
dc.subject.mesh	Leukocytes, Mononuclear
dc.subject.mesh	Male
dc.subject.mesh	Materials Testing
dc.subject.mesh	Middle Aged
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Particle Size
dc.subject.mesh	Polyethylenes
dc.subject.mesh	Young Adult
dc.title	The biological response to nanometre-sized polymer particles.
dc.type	Journal Article
utslib.citation.volume	23
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2021-07-21T07:25:48Z
pubs.publication-status	Published
pubs.volume	23

Abstract:

Recently, nanometre-sized UHMWPE particles generated from hip and knee replacements have been identified in vitro and in vivo. UHMWPE particles in the 0.1-1.0μm size range have been shown to be more biologically active than larger particles, provoking an inflammatory response implicated in late aseptic loosening of total joint replacements. The biological activity of nanometre-sized particles has not previously been studied. The biological response to clinically-relevant UHMWPE wear particles including nanometre-sized and micrometre-sized, along with polystyrene particles (FluoSpheres 20nm, 60nm, 200nm and 1.0μm), and nanometre-sized model polyethylene particles (Ceridust 3615®), was determined in terms of osteolytic cytokine release from primary human peripheral blood mononuclear cells (PBMNCs). Nanometre-sized UHMWPE wear particles, nanometre-sized Ceridust 3615® and 20nm FluoSpheres had no significant effect on TNF-α, IL-1β, IL-6 and IL-8 release from PBMNCs at a concentration of 100μm(3) particles per cell after 12 and 24h. The micrometre-size UHMWPE wear particles (0.1-1.0μm) and 60nm, 200nm and 1.0μm FluoSpheres caused significantly elevated osteolytic cytokine release from PBMNCs. These results indicated that particles below circa 50nm fail to activate PBMNCs and that particle size, composition and morphology played a crucial role in cytokine release by particle stimulated macrophages.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/149906